Amino Acid Deletions in p6 Gag Domain of HIV-1 CRF07_BC Ameliorate Galectin-3 Mediated Enhancement in Viral Budding
HIV-1 CRF07_BC is a recombinant virus with amino acid (a.a.) deletions in p6 , which are overlapped with the Alix-binding domain. Galectin-3 (Gal3), a β-galactose binding lectin, has been reported to interact with Alix and regulate HIV-1 subtype B budding. This study aims to evaluate the role of Gal...
Saved in:
Published in | International journal of molecular sciences Vol. 21; no. 8 |
---|---|
Main Authors | , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Switzerland
21.04.2020
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | HIV-1 CRF07_BC is a recombinant virus with amino acid (a.a.) deletions in p6
, which are overlapped with the Alix-binding domain. Galectin-3 (Gal3), a β-galactose binding lectin, has been reported to interact with Alix and regulate HIV-1 subtype B budding. This study aims to evaluate the role of Gal3 in HIV-1 CRF07_BC infection and the potential effect of a.a. deletions on Gal3-mediated regulation. A total of 38 HIV-1+ injecting drug users (IDUs) were enrolled in the study. Viral characterization and correlation of Gal3 were validated. CRF07_BC containing 7 a.a. deletions and wild-type in the p6
(CRF07_BC-7d and -wt) were isolated and infectious clones were generated. Viral growth kinetic and budding assays using Jurkat-CCR5/Jurkat-CCR5-Gal3 cells infected with CRF07_BC were performed. Results indicate that 69.4% (25/38) of the recruited patients were identified as CRF07_BC, and CRF07_BC-7d was predominant. Slow disease progression and significantly higher plasma Gal3 were noted in CRF07_BC patients (
< 0.01). Results revealed that CRF07_BC infection resulted in Gal3 expression, which was induced by Tat. Growth dynamic and budding assays indicated that Gal3 expression in Jurkat-CCR5 cells significantly enhanced CRF07_BC-wt replication and budding (
< 0.05), while the promoting effect was ameliorated in CRF07_BC-7d. Co-immunoprecipitation found that deletions in the p6
reduced Gal-3-mediated enhancement of the Alix-Gag interaction. |
---|---|
AbstractList | HIV-1 CRF07_BC is a recombinant virus with amino acid (a.a.) deletions in p6
, which are overlapped with the Alix-binding domain. Galectin-3 (Gal3), a β-galactose binding lectin, has been reported to interact with Alix and regulate HIV-1 subtype B budding. This study aims to evaluate the role of Gal3 in HIV-1 CRF07_BC infection and the potential effect of a.a. deletions on Gal3-mediated regulation. A total of 38 HIV-1+ injecting drug users (IDUs) were enrolled in the study. Viral characterization and correlation of Gal3 were validated. CRF07_BC containing 7 a.a. deletions and wild-type in the p6
(CRF07_BC-7d and -wt) were isolated and infectious clones were generated. Viral growth kinetic and budding assays using Jurkat-CCR5/Jurkat-CCR5-Gal3 cells infected with CRF07_BC were performed. Results indicate that 69.4% (25/38) of the recruited patients were identified as CRF07_BC, and CRF07_BC-7d was predominant. Slow disease progression and significantly higher plasma Gal3 were noted in CRF07_BC patients (
< 0.01). Results revealed that CRF07_BC infection resulted in Gal3 expression, which was induced by Tat. Growth dynamic and budding assays indicated that Gal3 expression in Jurkat-CCR5 cells significantly enhanced CRF07_BC-wt replication and budding (
< 0.05), while the promoting effect was ameliorated in CRF07_BC-7d. Co-immunoprecipitation found that deletions in the p6
reduced Gal-3-mediated enhancement of the Alix-Gag interaction. |
Author | Lu, Po-Liang Yeh, Chun-Sheng Wang, Wen-Hung Chen, Yen-Hsu Urbina, Aspiro Nayim Yuan, Ruei-Yu Liu, Fu-Tong Lin, Chih-Yen Chen, Yi-Ming Arthur Wang, Sheng-Fan |
Author_xml | – sequence: 1 givenname: Wen-Hung orcidid: 0000-0002-4494-1123 surname: Wang fullname: Wang, Wen-Hung organization: Division of Infectious Disease, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung 80708, Taiwan – sequence: 2 givenname: Chun-Sheng surname: Yeh fullname: Yeh, Chun-Sheng organization: Department of Medical Laboratory Science and Biotechnology, Kaohsiung Medical University, Kaohsiung 80708, Taiwan – sequence: 3 givenname: Chih-Yen surname: Lin fullname: Lin, Chih-Yen organization: Department of Medical Laboratory Science and Biotechnology, Kaohsiung Medical University, Kaohsiung 80708, Taiwan – sequence: 4 givenname: Ruei-Yu surname: Yuan fullname: Yuan, Ruei-Yu organization: Department of Medical Laboratory Science and Biotechnology, Kaohsiung Medical University, Kaohsiung 80708, Taiwan – sequence: 5 givenname: Aspiro Nayim surname: Urbina fullname: Urbina, Aspiro Nayim organization: Center for Tropical Medicine and Infectious Disease, Kaohsiung Medical University, Kaohsiung 80708, Taiwan – sequence: 6 givenname: Po-Liang orcidid: 0000-0002-7423-6783 surname: Lu fullname: Lu, Po-Liang organization: Division of Infectious Disease, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung 80708, Taiwan – sequence: 7 givenname: Yen-Hsu surname: Chen fullname: Chen, Yen-Hsu organization: Division of Infectious Disease, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung 80708, Taiwan – sequence: 8 givenname: Yi-Ming Arthur surname: Chen fullname: Chen, Yi-Ming Arthur organization: Master Program in Clinical Pharmacogenomics and Pharmacoproteomics, College of Pharmacy, Taipei Medical University, Taipei 110, Taiwan – sequence: 9 givenname: Fu-Tong surname: Liu fullname: Liu, Fu-Tong organization: Institute of Biomedical Sciences, Academia Sinica 11529, Taiwan – sequence: 10 givenname: Sheng-Fan orcidid: 0000-0002-4183-3872 surname: Wang fullname: Wang, Sheng-Fan organization: Department of Medical Research, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung 80708, Taiwan |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/32326345$$D View this record in MEDLINE/PubMed |
BookMark | eNqFjs1qg0AUhYfSkp82r1DuCwijY5QsjYk1i25KyVamzo29YeaOOLrI28dCu-7q_PBxOGvxyJ7xQaziNEkiKbN8KdYhXKVMVLLdLcRSzSZT6XYlQuGIPRQtGTigxZE8ByCGPoM33cHBOz0nf4H6dI5iKD8qmTf7EgqHlvygR5w5i-1IHCl4R0NzZeDI35pbdMjjz9qZBm1hPxlD3L2Ip4u2ATe_-ixeq-NnWUf99OXQNP1ATg-35u-l-he4AxpMRx8 |
ContentType | Journal Article |
DBID | CGR CUY CVF ECM EIF NPM |
DatabaseName | Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed |
DatabaseTitle | MEDLINE MEDLINE with Full Text Medline Complete PubMed MEDLINE (Ovid) |
DatabaseTitleList | MEDLINE |
Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Biology |
EISSN | 1422-0067 |
ExternalDocumentID | 32326345 |
Genre | Journal Article |
GrantInformation_xml | – fundername: Ministry of Science and Technology, Taiwan grantid: 108-2320-B-037-035-MY3 |
GroupedDBID | --- 29J 2WC 3V. 53G 5GY 5VS 7X7 88E 8FE 8FG 8FH 8FI 8FJ 8G5 A8Z AADQD AAFWJ AAHBH ABDBF ABJCF ABUWG ACGFO ACIHN ACIWK ACPRK ADBBV AEAQA AENEX AFKRA AFZYC ALIPV ALMA_UNASSIGNED_HOLDINGS AOIJS AZQEC BAWUL BBNVY BCNDV BENPR BHPHI BPHCQ BVXVI CCPQU CGR CS3 CUY CVF D1I DIK DU5 DWQXO E3Z EBD EBS ECM EIF EJD ESTFP ESX F5P FRP FYUFA GNUQQ GROUPED_DOAJ GUQSH GX1 HCIFZ HH5 HMCUK HYE IAO ITC KB. KQ8 LK8 M1P M2O M48 M7P MODMG M~E NPM O5R O5S OK1 P2P PDBOC PIMPY PQQKQ PROAC PSQYO RIG RNS RPM TR2 TUS UKHRP ~8M |
ID | FETCH-pubmed_primary_323263453 |
IngestDate | Wed Oct 16 00:45:35 EDT 2024 |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 8 |
Keywords | HIV-1 p6Gag Alix Galectin-3 CRF07_BC |
Language | English |
LinkModel | OpenURL |
MergedId | FETCHMERGED-pubmed_primary_323263453 |
ORCID | 0000-0002-7423-6783 0000-0002-4183-3872 0000-0002-4494-1123 |
PMID | 32326345 |
ParticipantIDs | pubmed_primary_32326345 |
PublicationCentury | 2000 |
PublicationDate | 2020-Apr-21 |
PublicationDateYYYYMMDD | 2020-04-21 |
PublicationDate_xml | – month: 04 year: 2020 text: 2020-Apr-21 day: 21 |
PublicationDecade | 2020 |
PublicationPlace | Switzerland |
PublicationPlace_xml | – name: Switzerland |
PublicationTitle | International journal of molecular sciences |
PublicationTitleAlternate | Int J Mol Sci |
PublicationYear | 2020 |
SSID | ssj0023259 |
Score | 4.317897 |
Snippet | HIV-1 CRF07_BC is a recombinant virus with amino acid (a.a.) deletions in p6
, which are overlapped with the Alix-binding domain. Galectin-3 (Gal3), a... |
SourceID | pubmed |
SourceType | Index Database |
SubjectTerms | Adolescent Adult CD4 Lymphocyte Count Cell Line Female gag Gene Products, Human Immunodeficiency Virus - genetics gag Gene Products, Human Immunodeficiency Virus - metabolism Galectin 3 - blood Galectin 3 - metabolism HIV Infections - immunology HIV Infections - metabolism HIV Infections - virology HIV-1 - physiology Host-Pathogen Interactions Humans Male Middle Aged Models, Biological Sequence Deletion Viral Load Virus Release Young Adult |
Title | Amino Acid Deletions in p6 Gag Domain of HIV-1 CRF07_BC Ameliorate Galectin-3 Mediated Enhancement in Viral Budding |
URI | https://www.ncbi.nlm.nih.gov/pubmed/32326345 |
Volume | 21 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnZ3LT8IwGMAbIDHxYny_SQ_eSAlsYxtHICAa8SDI40Q26NyMFKLsoH-939fuFSKJelmWdWu2_Zr2e5eQG9t055zPXIaljZjheSZzKx7ubwKrk1YBDbqOCc69R7P7bNyPa-Ncrp-JWgrXbnn29WNeyX-owjXgilmyfyCbdAoX4Bz4whEIw_FXjBuLQCxLjVmA0cRYRTsOCzdLt84LCMcL0Pulqf5uyKql1lOnYk2bLXTTvGFq_hqGiIN2-0AwXfpsHBRA28LHsSDDBOD5YYBJ_M1wnixzr2n4e2pNzNSgWMR77paiBTYR3EeReXrEBeuGUXfSZKtcPX4oWN_nacNDEEUFBD6bpGlrkzDyX4U8YJMwa7vQMJCUqYToMlfzrQG6MK6Y2Qk5ukMNPDs7z8PvXy0kTB0kQVNXlSg3CmbHTXmS16sY5dkzEscSNNWwUGx8z4YyIYWKwT7Zi7QB2lBoD0iOi0Oyo_YH_TwiHxIwRcA0AUwDQVcmBcBUAaZLj0rANAZMU8A0BUxjwDQDGHuTgGkE-JgUO-1Bq8vUG09XqhzJNP4W_YQUxFLwM0Itw3a4Z4A0adYNzzLqrsc1bluWXYc529HOyemWTi62tlyS3RThFSms30N-DQLZ2i3KX_wNSC477A |
link.rule.ids | 315,786,790 |
linkProvider | Scholars Portal |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Amino+Acid+Deletions+in+p6+Gag+Domain+of+HIV-1+CRF07_BC+Ameliorate+Galectin-3+Mediated+Enhancement+in+Viral+Budding&rft.jtitle=International+journal+of+molecular+sciences&rft.au=Wang%2C+Wen-Hung&rft.au=Yeh%2C+Chun-Sheng&rft.au=Lin%2C+Chih-Yen&rft.au=Yuan%2C+Ruei-Yu&rft.date=2020-04-21&rft.eissn=1422-0067&rft.volume=21&rft.issue=8&rft_id=info%3Apmid%2F32326345&rft.externalDocID=32326345 |