Role of RNase H enzymes in maintaining genome stability in Escherichia coli expressing a steric-gate mutant of pol V ICE391

• Published in: DNA repair Vol. 84; p. 102685
• Main Authors: Walsh, Erin, Henrikus, Sarah S, Vaisman, Alexandra, Makiela-Dzbenska, Karolina, Armstrong, Thomas J, Łazowski, Krystian, McDonald, John P, Goodman, Myron F, van Oijen, Antoine M, Jonczyk, Piotr, Fijalkowska, Iwona J, Robinson, Andrew, Woodgate, Roger
• Format: Journal Article
• Language: English
• Published: Netherlands 01.12.2019
• Subjects: DNA Repair; DNA-Directed DNA Polymerase - chemistry; DNA-Directed DNA Polymerase - genetics; DNA-Directed DNA Polymerase - metabolism; Escherichia coli; Escherichia coli Proteins - chemistry; Escherichia coli Proteins - genetics; Escherichia coli Proteins - metabolism; Genomic Instability; Mutation; Protein Domains; Ribonucleotides - genetics; Ribonucleotides - metabolism; Translation; Ribonucleotide excision repair; DNA polymerase V; Y-family DNA polymerase

pol V (RumA' B) is a low-fidelity polymerase that promotes considerably higher levels of spontaneous "SOS-induced" mutagenesis than the related E. coli pol V (UmuD' C). The molecular basis for the enhanced mutagenesis was previously unknown. Using single molecule fluorescence mic...