Quantitative determination of phosphoserine by high-performance liquid chromatography as the phenylthiocarbamyl-S-ethylcysteine. Application to picomolar amounts of peptides and proteins

A method is described that permits the phosphoserine content of proteins and peptides to be determined in picomolar amounts. A micro-batch reaction first converts phosphoserine into S-ethylcysteine. Hydrolysis with 6 M hydrochloric acid then yields the free amino acid, which is coupled with phenyl i...

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Published inJournal of chromatography Vol. 397; p. 113
Main Authors Meyer, H E, Swiderek, K, Hoffmann-Posorske, E, Korte, H, Heilmeyer, Jr, L M
Format Journal Article
LanguageEnglish
Published Netherlands 26.06.1987
Subjects
Amino Acids - analysis
Animals
Cattle
Chromatography, High Pressure Liquid
Cysteine - analogs & derivatives
Indicators and Reagents
Oligopeptides - analysis
Peptides - analysis
Phosphorylation
Phosphoserine - analysis
Proteins - analysis
Serine - analogs & derivatives
Troponin - analysis
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Abstract A method is described that permits the phosphoserine content of proteins and peptides to be determined in picomolar amounts. A micro-batch reaction first converts phosphoserine into S-ethylcysteine. Hydrolysis with 6 M hydrochloric acid then yields the free amino acid, which is coupled with phenyl isothiocyanate to give the corresponding phenylthiocarbamylamino acid. This derivative is determined quantitatively in the range 10-20 pmol by reversed-phase high-performance liquid chromatography. The method works well with either small peptides or proteins in the low picomole range.
AbstractList A method is described that permits the phosphoserine content of proteins and peptides to be determined in picomolar amounts. A micro-batch reaction first converts phosphoserine into S-ethylcysteine. Hydrolysis with 6 M hydrochloric acid then yields the free amino acid, which is coupled with phenyl isothiocyanate to give the corresponding phenylthiocarbamylamino acid. This derivative is determined quantitatively in the range 10-20 pmol by reversed-phase high-performance liquid chromatography. The method works well with either small peptides or proteins in the low picomole range.
Author Korte, H
Hoffmann-Posorske, E
Meyer, H E
Swiderek, K
Heilmeyer, Jr, L M
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  fullname: Heilmeyer, Jr, L M
BackLink https://www.ncbi.nlm.nih.gov/pubmed/3116018$$D View this record in MEDLINE/PubMed
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PublicationTitle Journal of chromatography
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Snippet A method is described that permits the phosphoserine content of proteins and peptides to be determined in picomolar amounts. A micro-batch reaction first...
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StartPage 113
SubjectTerms Amino Acids - analysis
Animals
Cattle
Chromatography, High Pressure Liquid
Cysteine - analogs & derivatives
Indicators and Reagents
Oligopeptides - analysis
Peptides - analysis
Phosphorylation
Phosphoserine - analysis
Proteins - analysis
Serine - analogs & derivatives
Troponin - analysis
Title Quantitative determination of phosphoserine by high-performance liquid chromatography as the phenylthiocarbamyl-S-ethylcysteine. Application to picomolar amounts of peptides and proteins
URI https://www.ncbi.nlm.nih.gov/pubmed/3116018
Volume 397
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