Augmentation of dietary glucosylceramide hydrolysis by the novel bacterium Glucerabacter canisensis NATH-2371 T

The purpose of this study was to evaluate the effects of intragastrical administration of Glucerabacter canisensis NATH-2371 on glucosylceramide (GluCer) digestion in mice. Although G. canisensis was unable to utilize starch and cellulose, coculture of G. canisensis with mouse fecal bacteria greatly...

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Published inBioscience, biotechnology, and biochemistry Vol. 82; no. 12; p. 2191
Main Authors Kawata, Misho, Suzuki, Masato, Akutsu, Shoko, Kawahara, Natsuki, Tsukamoto, Ami, Nobukawa, Shohei, Isozaki, Ryohei, Yuyama, Seika, Asanuma, Narito
Format Journal Article
LanguageEnglish
Published England 01.12.2018
Subjects
Animals
Clostridiales - metabolism
Dietary Fats - metabolism
Feces - microbiology
Glucosylceramides - metabolism
Hydrolysis
Male
Mice
Mice, Inbred ICR
Probiotics
Intestinal bacteria
Probiotics
Ceramide
Glucosylceramide
Mice
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Summary:The purpose of this study was to evaluate the effects of intragastrical administration of Glucerabacter canisensis NATH-2371 on glucosylceramide (GluCer) digestion in mice. Although G. canisensis was unable to utilize starch and cellulose, coculture of G. canisensis with mouse fecal bacteria greatly increased GluCer hydrolysis in polysaccharide medium, indicating that G. canisensis grew in competition with other intestinal bacteria. Although most of the administered G. canisensis cells were detected in feces, some cells were present in the colorectum contents, which had GluCer-hydrolyzing activity. These results indicate that G. canisensis can viably transit through the mouse gut. Administration of G. canisensis to mice fed diets supplemented with GluCer or GluCer-containing foods significantly enhanced GluCer hydrolysis. Since G. canisensis did not show acute toxicity, it may be useful as a probiotic to augment GluCer hydrolysis in the large intestine. Abbreviations: GluCer: glucosylceramide; KPi: potassium phosphate buffer; C-M: chloroform-methanol.
ISSN:1347-6947