Local and systemic effects of BdipTX-I, a Lys-49 phospholipase A 2 isolated from Bothrops diporus snake venom

• Published in: Toxicon (Oxford) Vol. 141; p. 55
• Main Authors: Teixera, Leda Fabiélen, de Carvalho, Letícia Helena, de Castro, Onássis Boeri, Bastos, Jéssica Silva Félix, Néry, Neriane Monteiro, Oliveira, George Azevedo, Kayano, Anderson Makoto, Soares, Andreimar Martins, Zuliani, Juliana Pavan
• Format: Journal Article
• Language: English
• Published: England 01.01.2018
• Subjects: Amino Acid Sequence; Animals; Bothrops; Crotalid Venoms - enzymology; Edema; Kidney - drug effects; Liver - drug effects; Mice; Muscle, Skeletal - drug effects; Muscle, Skeletal - pathology; Phospholipases A2 - toxicity; Proteinuria; Uremia; Bothrops diporus; Oedema; Phospholipase A; Myotoxicity; Systemic effect
• ISSN: 1879-3150

The present work aimed to isolate a basic phospholipase A (PLA ) from Bothrops diporus snake venom (BdV), evaluate and compare the myotoxic and oedema-inducing activities, as well as the systemic effects caused by both the isolated PLA and BdV on Swiss mice. A Lys-49 PLA (BdipTX-I) was obtained through two chromatographic steps: an ion-exchange and a reverse phase. The local (oedema and myotoxicity) and systemic (hepatic and renal functions) effects were then assessed for BdipTX-I and BdV. Results showed that the oedema-inducing activity was significant in all tested doses (5 and 20 μg/paw) for both BdipTX-I and BdV. Myotoxicity was evaluated by the increase of serum CK, CK-MB and LDH, and results showed that BdV effect is more prominent than BdipTX-I effect. The systemic effects were evaluated by determining specific laboratory markers: AST, ALT, GGT, ALP, urea, creatinine, protein and calcium. BdipTX-I and BdV were able to induce renal changes in the experimental model, leading to proteinuria (induced both by BdipTX-I and by BdV) and uremia (induced only by BdV). Thus, it is concluded that the systemic effects of BdV and BdipTX-I occur differently.