Characterization of a heat-tolerant Chlorella sp. GD mutant with enhanced photosynthetic CO 2 fixation efficiency and its implication as lactic acid fermentation feedstock
Fermentative production of lactic acid from algae-based carbohydrates devoid of lignin has attracted great attention for its potential as a suitable alternative substrate compared to lignocellulosic biomass. A sp. GD mutant with enhanced thermo-tolerance was obtained by mutagenesis using -methyl- ...
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Published in | Biotechnology for biofuels Vol. 10; p. 214 |
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Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
England
2017
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Subjects | |
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Abstract | Fermentative production of lactic acid from algae-based carbohydrates devoid of lignin has attracted great attention for its potential as a suitable alternative substrate compared to lignocellulosic biomass.
A
sp. GD mutant with enhanced thermo-tolerance was obtained by mutagenesis using
-methyl-
'-nitro-
-nitrosoguanidine to overcome outdoor high-temperature inhibition and it was used as a feedstock for fermentative lactic acid production. The indoor experiments showed that biomass, reducing sugar content, photosynthetic O
evolution rate, photosystem II activity (
/
and
'/
'), and chlorophyll content increased as temperature, light intensity, and CO
concentration increased. The mutant showed similar DIC affinity and initial slope of photosynthetic light response curve (α) as that of the wild type but had higher dissolved inorganic carbon (DIC) utilization capacity and maximum photosynthesis rate (
). Moreover, the PSII activity (
'/
') in the mutant remained normal without acclimation process after being transferred to photobioreactor. This suggests that efficient utilization of incident high light and enhanced carbon fixation with its subsequent flux to carbohydrates accumulation in the mutant contributes to higher sugar and biomass productivity under enriched CO
condition. The mutant was cultured outdoors in a photobioreactor with 6% CO
aeration in hot summer season in southern Taiwan. The harvested biomass was subjected to separate hydrolysis and fermentation (SHF) for lactic acid production with carbohydrate concentration equivalent to 20 g/L glucose using the lactic acid-producing bacterium
23. The conversion rate and yield of lactic acid were 80% and 0.43 g/g
biomass, respectively.
These results demonstrated that the thermo-tolerant
mutant with high photosynthetic efficiency and biomass productivity under hot outdoor condition is an efficient fermentative feedstock for large-scale lactic acid production. |
---|---|
AbstractList | Fermentative production of lactic acid from algae-based carbohydrates devoid of lignin has attracted great attention for its potential as a suitable alternative substrate compared to lignocellulosic biomass.
A
sp. GD mutant with enhanced thermo-tolerance was obtained by mutagenesis using
-methyl-
'-nitro-
-nitrosoguanidine to overcome outdoor high-temperature inhibition and it was used as a feedstock for fermentative lactic acid production. The indoor experiments showed that biomass, reducing sugar content, photosynthetic O
evolution rate, photosystem II activity (
/
and
'/
'), and chlorophyll content increased as temperature, light intensity, and CO
concentration increased. The mutant showed similar DIC affinity and initial slope of photosynthetic light response curve (α) as that of the wild type but had higher dissolved inorganic carbon (DIC) utilization capacity and maximum photosynthesis rate (
). Moreover, the PSII activity (
'/
') in the mutant remained normal without acclimation process after being transferred to photobioreactor. This suggests that efficient utilization of incident high light and enhanced carbon fixation with its subsequent flux to carbohydrates accumulation in the mutant contributes to higher sugar and biomass productivity under enriched CO
condition. The mutant was cultured outdoors in a photobioreactor with 6% CO
aeration in hot summer season in southern Taiwan. The harvested biomass was subjected to separate hydrolysis and fermentation (SHF) for lactic acid production with carbohydrate concentration equivalent to 20 g/L glucose using the lactic acid-producing bacterium
23. The conversion rate and yield of lactic acid were 80% and 0.43 g/g
biomass, respectively.
These results demonstrated that the thermo-tolerant
mutant with high photosynthetic efficiency and biomass productivity under hot outdoor condition is an efficient fermentative feedstock for large-scale lactic acid production. |
Author | Tseng, Yu-Fei Chow, Te-Jin Chen, Yi-Chuan Chen, Chun-Yen Lin, Chih-Sheng Chang, Jo-Shu Su, Hsiang-Yen Lee, Tse-Min Cheng, Chieh-Lun |
Author_xml | – sequence: 1 givenname: Tse-Min surname: Lee fullname: Lee, Tse-Min organization: Doctoral Degree Program in Marine Biotechnology, National Sun Yat-sen University, Kaohsiung, 80424 Taiwan – sequence: 2 givenname: Yu-Fei surname: Tseng fullname: Tseng, Yu-Fei organization: Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung, 80424 Taiwan – sequence: 3 givenname: Chieh-Lun surname: Cheng fullname: Cheng, Chieh-Lun organization: Department of Chemical Engineering, National Cheng-Kung University, Tainan, 70146 Taiwan – sequence: 4 givenname: Yi-Chuan surname: Chen fullname: Chen, Yi-Chuan organization: Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung, 80424 Taiwan – sequence: 5 givenname: Chih-Sheng surname: Lin fullname: Lin, Chih-Sheng organization: Department of Biological Science and Technology, National Chiao Tung University, Hsinchu, 30068 Taiwan – sequence: 6 givenname: Hsiang-Yen surname: Su fullname: Su, Hsiang-Yen organization: Department of Biotechnology, Fooyin University, Kaohsiung, 83102 Taiwan – sequence: 7 givenname: Te-Jin surname: Chow fullname: Chow, Te-Jin organization: Department of Biotechnology, Fooyin University, Kaohsiung, 83102 Taiwan – sequence: 8 givenname: Chun-Yen surname: Chen fullname: Chen, Chun-Yen organization: University Center for Bioscience and Biotechnology, National Cheng-Kung University, Tainan, 70146 Taiwan – sequence: 9 givenname: Jo-Shu orcidid: 0000-0003-3469-9553 surname: Chang fullname: Chang, Jo-Shu organization: Department of Chemical Engineering, National Cheng-Kung University, Tainan, 70146 Taiwan |
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Keywords | CO2 utilization efficiency Chlorella sp N-methyl-N′-nitro-N-nitrosoguanidine (MNNG) Mutagenesis Lactic acid Light conversion efficiency Photosynthesis |
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Title | Characterization of a heat-tolerant Chlorella sp. GD mutant with enhanced photosynthetic CO 2 fixation efficiency and its implication as lactic acid fermentation feedstock |
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