Danggui Buxue decoction promotes angiogenesis by up-regulation of VEGFR 1/2 expressions and down-regulation of sVEGFR 1/2 expression in myocardial infarction rat

The traditional herbal compound Danggui Buxue decoction (DBD), has long been used for the prevention and treatment of cardiovascular diseases, however, the underlying molecular mechanism for its effect remains still unknown. So this study would to investigate the effect of DBD on cardiac damage indu...

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Published inJournal of the Chinese Medical Association Vol. 81; no. 1; p. 37
Main Authors Hu, Guanying, Yang, Peng, Zeng, Yu, Zhang, Sanyin, Song, Jiage
Format Journal Article
LanguageEnglish
Published Netherlands 01.01.2018
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Abstract The traditional herbal compound Danggui Buxue decoction (DBD), has long been used for the prevention and treatment of cardiovascular diseases, however, the underlying molecular mechanism for its effect remains still unknown. So this study would to investigate the effect of DBD on cardiac damage induced by myocardial infarction (MI) challenge. SD Rats with ligation of left anterior descending (LAD) coronary artery were randomly divided into MI, MI plus Betaloc Zok, MI plus DBD high dose, and MI plus DBD low dose group, together with sham-operated group. After corresponding treatment for consecutive 4 weeks, cardiac function was evaluated by hemodynamics with the method of pressure-volume conduit system. Cardiac histological morphology, microvascular density and the expressions of VEGF and VEGFR mRNA and their relative protein including VEGF, membranous VEGFR (VEGFR ), soluble VEGFR (sVEGFR ), VEGFR , and sVEGFR were examined by hematoxylin & eosin staining, immunohistochemical staining and quantitative polymerase chain reaction and western blot assay, respectively. It showed that a significant impaired cardiac function and a remarkably inducible increase in fibrotic scar formation, microvascular density and VEGF mRNA expressions in MI rats. While DBD treatment could markedly boost cardiac angiogenesis further, hinder fibrotic scar formation, and improve declined cardiac function. Apart from the up-regulation of VEGF mRNA and VEGF and the down-regulation of sVEGFR , high dose of DBD dedicated to increasing VEGFR mRNA and VEGFR expression, while low dose to elevating VEGFR mRNA and VEGFR expression. The present study demonstrated that DBD could accelerate cardiac angiogenesis, restrain fibrous scar formation and thus ameliorate cardiac function in post-MI, via the active regulation of VEGF/VEGFRs signaling pathway.
AbstractList The traditional herbal compound Danggui Buxue decoction (DBD), has long been used for the prevention and treatment of cardiovascular diseases, however, the underlying molecular mechanism for its effect remains still unknown. So this study would to investigate the effect of DBD on cardiac damage induced by myocardial infarction (MI) challenge. SD Rats with ligation of left anterior descending (LAD) coronary artery were randomly divided into MI, MI plus Betaloc Zok, MI plus DBD high dose, and MI plus DBD low dose group, together with sham-operated group. After corresponding treatment for consecutive 4 weeks, cardiac function was evaluated by hemodynamics with the method of pressure-volume conduit system. Cardiac histological morphology, microvascular density and the expressions of VEGF and VEGFR mRNA and their relative protein including VEGF, membranous VEGFR (VEGFR ), soluble VEGFR (sVEGFR ), VEGFR , and sVEGFR were examined by hematoxylin & eosin staining, immunohistochemical staining and quantitative polymerase chain reaction and western blot assay, respectively. It showed that a significant impaired cardiac function and a remarkably inducible increase in fibrotic scar formation, microvascular density and VEGF mRNA expressions in MI rats. While DBD treatment could markedly boost cardiac angiogenesis further, hinder fibrotic scar formation, and improve declined cardiac function. Apart from the up-regulation of VEGF mRNA and VEGF and the down-regulation of sVEGFR , high dose of DBD dedicated to increasing VEGFR mRNA and VEGFR expression, while low dose to elevating VEGFR mRNA and VEGFR expression. The present study demonstrated that DBD could accelerate cardiac angiogenesis, restrain fibrous scar formation and thus ameliorate cardiac function in post-MI, via the active regulation of VEGF/VEGFRs signaling pathway.
Author Zeng, Yu
Yang, Peng
Hu, Guanying
Song, Jiage
Zhang, Sanyin
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  organization: TCM Qi & Blood Functional Laboratory, College of Basic Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, China
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Issue 1
Keywords VEGFRs
Myocardium infraction
Cardiac function
VEGF
Cardiac angiogenesis
Danggui buxue decoction
Language English
License Copyright © 2017. Published by Elsevier Taiwan LLC.
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PublicationTitle Journal of the Chinese Medical Association
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Snippet The traditional herbal compound Danggui Buxue decoction (DBD), has long been used for the prevention and treatment of cardiovascular diseases, however, the...
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StartPage 37
SubjectTerms Animals
Drugs, Chinese Herbal - pharmacology
Hemodynamics - drug effects
Male
Myocardial Infarction - drug therapy
Myocardial Infarction - pathology
Myocardial Infarction - physiopathology
Myocardium - pathology
Neovascularization, Physiologic - drug effects
Rats
Rats, Sprague-Dawley
Vascular Endothelial Growth Factor A - genetics
Vascular Endothelial Growth Factor Receptor-1 - genetics
Vascular Endothelial Growth Factor Receptor-2 - genetics
Title Danggui Buxue decoction promotes angiogenesis by up-regulation of VEGFR 1/2 expressions and down-regulation of sVEGFR 1/2 expression in myocardial infarction rat
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Volume 81
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