A forward genetic screen identifies a negative regulator of rapid Ca 2+ -dependent cell egress (MS1) in the intracellular parasite Toxoplasma gondii

• Published in: The Journal of biological chemistry Vol. 292; no. 18; p. 7662
• Main Authors: McCoy, James M, Stewart, Rebecca J, Uboldi, Alessandro D, Li, Dongdi, Schröder, Jan, Scott, Nicollas E, Papenfuss, Anthony T, Lehane, Adele M, Foster, Leonard J, Tonkin, Christopher J
• Format: Journal Article
• Language: English
• Published: United States 05.05.2017
• Subjects: Calcium Signaling - physiology; Calcium-Binding Proteins - genetics; Calcium-Binding Proteins - metabolism; Phosphorylation - physiology; Protein Kinases - genetics; Protein Kinases - metabolism; Protozoan Proteins - genetics; Protozoan Proteins - metabolism; Toxoplasma - genetics; Toxoplasma - metabolism; molecular genetics; parasite; Toxoplasma gondii; signaling; apicomplexa; forward genetic screen; proteomics; Ca2+ signalling; host cell egress

, like all apicomplexan parasites, uses Ca signaling pathways to activate gliding motility to power tissue dissemination and host cell invasion and egress. A group of "plant-like" Ca -dependent protein kinases (CDPKs) transduces cytosolic Ca flux into enzymatic activity, but how they funct...