DNA-binding Specificity Is a Major Determinant of the Activity and Toxicity of Zinc-finger Nucleases

• Published in: Molecular therapy Vol. 16; no. 2; p. 352
• Main Authors: Cornu, Tatjana I, Thibodeau-Beganny, Stacey, Guhl, Eva, Alwin, Stephen, Eichtinger, Magdalena, Joung, J K, Cathomen, Toni
• Format: Journal Article
• Language: English
• Published: United States 01.02.2008

The engineering of proteins to manipulate cellular genomes has developed into a promising technology for biomedical research, including gene therapy. In particular, zinc-finger nucleases (ZFNs), which consist of a nonspecific endonuclease domain tethered to a tailored zinc-finger (ZF) DNA-binding do...