Epitope analysis of Japanese cedar pollen allergen Cry j2 with a human IgM class monoclonal antibody 404-117
Japanese cedar pollen allergen Cry j2 is a causal allergen of seasonal pollinosis in Japan. To analyze B cell epitopes of Cry j2, we established two human-mouse hybridomas secreting IgM class human monoclonal antibodies to Cry j2. A pin-peptide enzyme-linked immunosorbent assay with synthesized icos...
Saved in:
Published in | Human antibodies Vol. 25; no. 1-2; p. 17 |
---|---|
Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Netherlands
2017
|
Subjects | |
Online Access | Get more information |
Cover
Loading…
Abstract | Japanese cedar pollen allergen Cry j2 is a causal allergen of seasonal pollinosis in Japan. To analyze B cell epitopes of Cry j2, we established two human-mouse hybridomas secreting IgM class human monoclonal antibodies to Cry j2. A pin-peptide enzyme-linked immunosorbent assay with synthesized icosa peptides showed that 404-117 monoclonal antibody bound to peptides #11-13 with cry j2 amino acid sequence of 101F-L140. Detailed analysis with octa peptides and alanine substituted peptides indicated that an amino acid sequence of 118FKVD121 was an essential for antibody binding. When K119 (Asn) was substituted with alanine, 404-117 monoclonal antibody did not bind to the alanine substituted peptide. We concluded that the 118FKVD121 sequence might have a very important role in early recognition by Cry j2-specific B cells, which could act as antigen presenting cells. |
---|---|
AbstractList | Japanese cedar pollen allergen Cry j2 is a causal allergen of seasonal pollinosis in Japan. To analyze B cell epitopes of Cry j2, we established two human-mouse hybridomas secreting IgM class human monoclonal antibodies to Cry j2. A pin-peptide enzyme-linked immunosorbent assay with synthesized icosa peptides showed that 404-117 monoclonal antibody bound to peptides #11-13 with cry j2 amino acid sequence of 101F-L140. Detailed analysis with octa peptides and alanine substituted peptides indicated that an amino acid sequence of 118FKVD121 was an essential for antibody binding. When K119 (Asn) was substituted with alanine, 404-117 monoclonal antibody did not bind to the alanine substituted peptide. We concluded that the 118FKVD121 sequence might have a very important role in early recognition by Cry j2-specific B cells, which could act as antigen presenting cells. |
Author | Chiba, Seiya Shimmoto, Michie Shinmoto, Hiroshi Naganawa, Yasunori Kumazawa, Ayane Yokoyama, Haruka |
Author_xml | – sequence: 1 givenname: Seiya surname: Chiba fullname: Chiba, Seiya organization: Tamagawa University, College of Agriculture, Machida, Tokyo, Japan – sequence: 2 givenname: Haruka surname: Yokoyama fullname: Yokoyama, Haruka organization: Tamagawa University, College of Agriculture, Machida, Tokyo, Japan – sequence: 3 givenname: Ayane surname: Kumazawa fullname: Kumazawa, Ayane organization: Tamagawa University, College of Agriculture, Machida, Tokyo, Japan – sequence: 4 givenname: Michie surname: Shimmoto fullname: Shimmoto, Michie organization: Tokyo Metropolitan Institute of Medical Science, Setagaya-ku, Tokyo, Japan – sequence: 5 givenname: Yasunori surname: Naganawa fullname: Naganawa, Yasunori organization: Tamagawa University, College of Agriculture, Machida, Tokyo, Japan – sequence: 6 givenname: Hiroshi surname: Shinmoto fullname: Shinmoto, Hiroshi organization: Tamagawa University, College of Agriculture, Machida, Tokyo, Japan |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/27858705$$D View this record in MEDLINE/PubMed |
BookMark | eNqFjr0OgjAUhRujUfx5BXNfgEQI2DoTjJq4ObiRCxSoKW3TQgxvbwedXc45w5cvZ03mSis-I0HEaBqy4-m5JKuYspTRQxoQmRsxaMMBFcrJCQe6gRsaVNxxqHiNFoyWkitAn7b1I7MTvGJ4i6EDhG7sUcG1vUMl0TnotdKV1F7nnYModT1BckjCKKJbsmhQOr779obsz_kju4RmLHteF8aKHu1U_O7Ff4EPJLFD7g |
ContentType | Journal Article |
DBID | CGR CUY CVF ECM EIF NPM |
DatabaseName | Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed |
DatabaseTitle | MEDLINE MEDLINE with Full Text Medline Complete PubMed MEDLINE (Ovid) |
DatabaseTitleList | MEDLINE |
Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database |
DeliveryMethod | no_fulltext_linktorsrc |
EISSN | 1875-869X |
ExternalDocumentID | 27858705 |
Genre | Journal Article |
GroupedDBID | CGR CUY CVF ECM EIF NPM |
ID | FETCH-pubmed_primary_278587052 |
IngestDate | Fri Sep 17 20:16:55 EDT 2021 |
IsPeerReviewed | false |
IsScholarly | false |
Issue | 1-2 |
Keywords | allergen Human monoclonal antibody Japanese cedar pollen epitope IgM |
Language | English |
LinkModel | OpenURL |
MergedId | FETCHMERGED-pubmed_primary_278587052 |
PMID | 27858705 |
ParticipantIDs | pubmed_primary_27858705 |
PublicationCentury | 2000 |
PublicationDate | 2017-00-00 |
PublicationDateYYYYMMDD | 2017-01-01 |
PublicationDate_xml | – year: 2017 text: 2017-00-00 |
PublicationDecade | 2010 |
PublicationPlace | Netherlands |
PublicationPlace_xml | – name: Netherlands |
PublicationTitle | Human antibodies |
PublicationTitleAlternate | Hum Antibodies |
PublicationYear | 2017 |
Score | 3.3157496 |
Snippet | Japanese cedar pollen allergen Cry j2 is a causal allergen of seasonal pollinosis in Japan. To analyze B cell epitopes of Cry j2, we established two... |
SourceID | pubmed |
SourceType | Index Database |
StartPage | 17 |
SubjectTerms | Allergens - chemistry Allergens - immunology Amino Acid Sequence Animals Antibodies, Monoclonal - biosynthesis Antibody Specificity Antigen-Presenting Cells - immunology Antigen-Presenting Cells - pathology Antigens, Plant - chemistry Antigens, Plant - immunology B-Lymphocytes - immunology B-Lymphocytes - pathology Binding Sites Cryptomeria - chemistry Cryptomeria - immunology Epitopes - chemistry Epitopes - immunology Humans Hybridomas - immunology Hybridomas - secretion Immunoglobulin M - biosynthesis Japan Mice Peptides - chemistry Peptides - immunology Plant Proteins - chemistry Plant Proteins - immunology Pollen - chemistry Pollen - immunology Protein Binding Rhinitis, Allergic, Seasonal - chemically induced Rhinitis, Allergic, Seasonal - immunology Rhinitis, Allergic, Seasonal - pathology |
Title | Epitope analysis of Japanese cedar pollen allergen Cry j2 with a human IgM class monoclonal antibody 404-117 |
URI | https://www.ncbi.nlm.nih.gov/pubmed/27858705 |
Volume | 25 |
hasFullText | |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnZ1La8JAEICX2l56KS19P2QOvYUUjYmJxyKKCu1FC95kN48aH0kQS4m_vjO7iQlS6eMSZEfCZj8zzszOzDL26Am7gX4C1z1KDjMtQ-itmsn1hiWcOhccrVSZbfHa7L2Zg7E1LhIyZXXJWjy5m2_rSv5DFceQK1XJ_oHs9qY4gJ-RL16RMF5_xbiT4PuY0AZA0VlkgP9-dKqk5voetbGmwECk0YkpVGaptVepNjOykrbshL7--4vmkhWt4cxjdyGDg7jioYi9VDOp5aCqfMytWBX5z75RykJsT0MhjdGhH6a8UCjzOOVLKejx1cd8K6H87g3_VKHdlBc7_MNpiKsoT3iSif2hX45O1O1SdMJXGhUdIt1pyvNytypX1TrnP62s_lFpUPU8JXjJUtIzbMdC3WL9LN3pn52LKqxiO6QDO_0utYDOhnfcCWlWjE7ZSeYPwLOCe8YO_OicLTKwkIOFOIAcLEiwoMBCDhYQLMwMILDAQYIFBAsSLBRgIQcLGdgLVu12Ru2eriY4SVT_kUk-deOSHUZx5F8zqFkBefpeYLUEbeVy36BeeJ5rCNGqcXHDrvbc5Hav5I4dF0Tv2VGAb43_gCbYWlTlKn4BjKw7xA |
link.rule.ids | 786 |
linkProvider | Clarivate |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Epitope+analysis+of+Japanese+cedar+pollen+allergen+Cry+j2+with+a+human+IgM+class+monoclonal+antibody+404-117&rft.jtitle=Human+antibodies&rft.au=Chiba%2C+Seiya&rft.au=Yokoyama%2C+Haruka&rft.au=Kumazawa%2C+Ayane&rft.au=Shimmoto%2C+Michie&rft.date=2017-01-01&rft.eissn=1875-869X&rft.volume=25&rft.issue=1-2&rft.spage=17&rft_id=info%3Apmid%2F27858705&rft_id=info%3Apmid%2F27858705&rft.externalDocID=27858705 |