Molecular and structural evaluation of dentin caries-like lesions produced by different artificial models
This study evaluated structural and molecular issues of dentin caries-like lesions produced by different artificial models (ACL) compared with natural caries lesions (NCL). One hundred twenty-four sound occlusal dentin blocks and 47 carious blocks were obtained and surface hardness was analyzed (SH1...
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Published in | Brazilian dental journal Vol. 24; no. 6; p. 610 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Brazil
01.11.2013
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Abstract | This study evaluated structural and molecular issues of dentin caries-like lesions produced by different artificial models (ACL) compared with natural caries lesions (NCL). One hundred twenty-four sound occlusal dentin blocks and 47 carious blocks were obtained and surface hardness was analyzed (SH1). They were assigned to groups according to ACL: GB: Biological; GC: Chemical; GIS: In situ; GNC: natural caries (control). Blocks from groups 1, 2 and 3 were submitted to caries lesion induction. NCL and ACL blocks were submitted to surface hardness (SH 2), FT-Raman and µEDXRF analysis. All blocks were longitudinally sectioned and one of the halves was submitted to cross-sectional hardness (CSH) and the other to SEM analysis. SH1 and SH2 data were submitted to t test (unpaired and paired, respectively), CSH and SEM data to two-way and one-way ANOVA respectively, and Tukey and t tests, respectively (p<0.05). Data from FT-Raman/µEDXRF were submitted to one-way ANOVA and Dunnett multiple-comparisons test (a=0.05). GB and GNC showed lowest SH2 values that were significantly different from GC and GIS. Regarding CSH, GB and GNC showed no significant difference between them. SEM showed similar caries lesion depth for GB and GNC, being significantly higher than for GC and GIS. µEDXRF showed similar values of calcium and phosphate for GB and GNC; GNC values were significantly different from GIS. No significant difference was found among the groups concerning phosphate, carbonate and CH bonds values. For collagen type I, GC values were significantly different compared to other groups. It may be concluded that caries-like lesions produced by GB were the closest model to NCL. |
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AbstractList | This study evaluated structural and molecular issues of dentin caries-like lesions produced by different artificial models (ACL) compared with natural caries lesions (NCL). One hundred twenty-four sound occlusal dentin blocks and 47 carious blocks were obtained and surface hardness was analyzed (SH1). They were assigned to groups according to ACL: GB: Biological; GC: Chemical; GIS: In situ; GNC: natural caries (control). Blocks from groups 1, 2 and 3 were submitted to caries lesion induction. NCL and ACL blocks were submitted to surface hardness (SH 2), FT-Raman and µEDXRF analysis. All blocks were longitudinally sectioned and one of the halves was submitted to cross-sectional hardness (CSH) and the other to SEM analysis. SH1 and SH2 data were submitted to t test (unpaired and paired, respectively), CSH and SEM data to two-way and one-way ANOVA respectively, and Tukey and t tests, respectively (p<0.05). Data from FT-Raman/µEDXRF were submitted to one-way ANOVA and Dunnett multiple-comparisons test (a=0.05). GB and GNC showed lowest SH2 values that were significantly different from GC and GIS. Regarding CSH, GB and GNC showed no significant difference between them. SEM showed similar caries lesion depth for GB and GNC, being significantly higher than for GC and GIS. µEDXRF showed similar values of calcium and phosphate for GB and GNC; GNC values were significantly different from GIS. No significant difference was found among the groups concerning phosphate, carbonate and CH bonds values. For collagen type I, GC values were significantly different compared to other groups. It may be concluded that caries-like lesions produced by GB were the closest model to NCL. |
Author | Soares, Luís Eduardo Silva Pascon, Fernanda Miori Rodrigues, Eliana Correr-Sobrinho, Lourenço Puppin-Rontani, Regina Maria Banzi, Éfani Caroline de Freitas Pacheco, Larissa Ferreira |
Author_xml | – sequence: 1 givenname: Larissa Ferreira surname: Pacheco fullname: Pacheco, Larissa Ferreira organization: Department of Pediatric Dentistry, Piracicaba School of Dentistry, UNICAMP - University of Campinas, PiracicabaSP, Brazil – sequence: 2 givenname: Éfani Caroline de Freitas surname: Banzi fullname: Banzi, Éfani Caroline de Freitas organization: Department of Pediatric Dentistry, Piracicaba School of Dentistry, UNICAMP - University of Campinas, PiracicabaSP, Brazil – sequence: 3 givenname: Eliana surname: Rodrigues fullname: Rodrigues, Eliana organization: Department of Pediatric Dentistry, Piracicaba School of Dentistry, UNICAMP - University of Campinas, PiracicabaSP, Brazil – sequence: 4 givenname: Luís Eduardo Silva surname: Soares fullname: Soares, Luís Eduardo Silva organization: Department of Dental Materials and Operative Dentistry, Vale do Paraíba University; School of Dentistry, Research and Development Institute, IP&D, Laboratory of Biomedical Vibrational Spectroscopy, LEVB. São José dos CamposSP, Brazil – sequence: 5 givenname: Fernanda Miori surname: Pascon fullname: Pascon, Fernanda Miori organization: Department of Pediatric Dentistry, Piracicaba School of Dentistry, UNICAMP - University of Campinas, PiracicabaSP, Brazil – sequence: 6 givenname: Lourenço surname: Correr-Sobrinho fullname: Correr-Sobrinho, Lourenço organization: Department of Restorative Dentistry, Dental Materials Division, Piracicaba School of Dentistry, UNICAMP - University of Campinas, PiracicabaSP, Brazil – sequence: 7 givenname: Regina Maria surname: Puppin-Rontani fullname: Puppin-Rontani, Regina Maria organization: Department of Pediatric Dentistry, Piracicaba School of Dentistry, UNICAMP - University of Campinas, PiracicabaSP, Brazil |
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SubjectTerms | Dental Caries - pathology Dental Enamel - pathology Dentin - pathology Hardness Humans In Vitro Techniques Microscopy, Electron, Scanning Molar, Third Spectrometry, X-Ray Emission Spectroscopy, Fourier Transform Infrared Surface Properties |
Title | Molecular and structural evaluation of dentin caries-like lesions produced by different artificial models |
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