DNA methylation analysis of human imprinted Loci by bisulfite genomic sequencing

• Published in: CSH protocols Vol. 2008; p. pdb.prot5046
• Main Authors: Angeles, Vanessa T, Reijo Pera, Renee A
• Format: Journal Article
• Language: English
• Published: United States 01.09.2008

INTRODUCTIONThe genomic imprinting mechanism regulates differential expression of paternally and maternally derived genes. During development, primordial germ cells (PGCs) have a unique methylation pattern which undergoes demethylation, both globally and at imprinted loci. Later, the methylation sta...