Immunodiagnosis of HIV infection in the transfusion service

Enzyme immunoassay (EIA) is the basic method of the laboratory diagnosis of HIV-infection used as the first component of donors' screening. The possibility of nonspecific reactions presents a significant shortcoming of this method. An algorithm involving three stages has been elaborated to inve...

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Bibliographic Details
Published inGematologiia i transfuziologiia Vol. 36; no. 5; p. 34
Main Authors Golosova, T V, Margolina, A N, Tupoleva, T A, Guliaeva, A A
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.05.1991
Subjects
AIDS Serodiagnosis
Blood Donors
Humans
Immunoenzyme Techniques
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Summary:Enzyme immunoassay (EIA) is the basic method of the laboratory diagnosis of HIV-infection used as the first component of donors' screening. The possibility of nonspecific reactions presents a significant shortcoming of this method. An algorithm involving three stages has been elaborated to investigate donors for anti-HIV antibodies and to verify seropositive samples. The first-stage screening is conducted with the use of Soviet test-reagents. Positive samples are assayed again in the Soviet EI-systems based on varying types of sorbed antigens. In case of questionable or positive reactions the sample is assayed in commercial imported test-systems (the second stage). The positive sera are assayed in the immunoblotting test (the third stage). The algorithm suggested provides a reliable control and is worthwhile economically. The causes of nonspecific reactions in EIA have been described.
ISSN:0234-5730