Participation of cyclic nucleotides, protein kinase A and C in realization of n-acylethanolamine action in human adrenocortical cells
The messenger mechanisms mediating N-acylethanolamines (NAE) regulatory signals in the adrenal cortex were studied. An analysis of the mechanisms of realization of NAE effects in the post-operation human adrenal cortex was carried out in vitro. Influence of NAE mix on cAMP and cGMP level, protein ki...
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Published in | Ukrains'kyi biokhimichnyi zhurnal (1999 ) Vol. 79; no. 5; p. 133 |
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Main Authors | , , , |
Format | Journal Article |
Language | Ukrainian |
Published |
Ukraine
01.09.2007
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Abstract | The messenger mechanisms mediating N-acylethanolamines (NAE) regulatory signals in the adrenal cortex were studied. An analysis of the mechanisms of realization of NAE effects in the post-operation human adrenal cortex was carried out in vitro. Influence of NAE mix on cAMP and cGMP level, protein kinase A and C activity in sub-cellular fraction of adrenocorticocytes and homogenates of conditionally normal adrenal cortex tissues was investigated. It was shown, that N-acylethanolamines treatment resulted in a decrease of cAMP level in adrenocortical cells. cGMP level is not changed in these conditions. The rise of protein kinase C activity was obtained in the membrane fraction after N-acylethanolamines in vitro treatment (3.3 microg/ml). Activity of cAMP-dependent protein kinase A significantly decreased in cytosol fraction of adrenocorticocytes. It was concluded, that steroid genesis activation is determined by protein kinase C activation, inhibition is determined by cAMP-dependent messenger system. |
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AbstractList | The messenger mechanisms mediating N-acylethanolamines (NAE) regulatory signals in the adrenal cortex were studied. An analysis of the mechanisms of realization of NAE effects in the post-operation human adrenal cortex was carried out in vitro. Influence of NAE mix on cAMP and cGMP level, protein kinase A and C activity in sub-cellular fraction of adrenocorticocytes and homogenates of conditionally normal adrenal cortex tissues was investigated. It was shown, that N-acylethanolamines treatment resulted in a decrease of cAMP level in adrenocortical cells. cGMP level is not changed in these conditions. The rise of protein kinase C activity was obtained in the membrane fraction after N-acylethanolamines in vitro treatment (3.3 microg/ml). Activity of cAMP-dependent protein kinase A significantly decreased in cytosol fraction of adrenocorticocytes. It was concluded, that steroid genesis activation is determined by protein kinase C activation, inhibition is determined by cAMP-dependent messenger system. |
Author | Mykosha, O S Levchuk, N I Hula, N M Kovzun, O I |
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SubjectTerms | Adrenal Cortex - drug effects Adrenal Cortex - enzymology Adrenal Cortex - metabolism Cells, Cultured Cyclic AMP - metabolism Cyclic AMP-Dependent Protein Kinases - metabolism Cyclic GMP - metabolism Ethanolamines - pharmacology Humans Protein Kinase C - metabolism Signal Transduction Subcellular Fractions - drug effects Subcellular Fractions - enzymology Subcellular Fractions - metabolism |
Title | Participation of cyclic nucleotides, protein kinase A and C in realization of n-acylethanolamine action in human adrenocortical cells |
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