Clinical significance of serum GPDA-F determined by immunoelectrophoresis in diagnosis of hepatocellular carcinoma

Serum fast band of glycylproline dipeptidyl aminopeptidase isoenzyme (GPDA-F) is useful to the diagnosis of hepatocellular carcinoma, especially for the cases without expression of alpha-fetoprotein (AFP). Polyacrylamide electrophoresis for detection of GPDA-F is relatively complicated and has limit...

Full description

Saved in:
Bibliographic Details
Published inAi zheng = Aizheng = Chinese journal of cancer Vol. 25; no. 2; p. 247
Main Authors Liu, Hong-Dao, Ni, Run-Zhou, Xiao, Ming-Bing, Li, Li-Ren, Hua, Guo-Ping, Kuai, Xiao-Ling
Format Journal Article
LanguageChinese
Published China 01.02.2006
Subjects
Aminopeptidases - blood
Animals
Carcinoma, Hepatocellular - diagnosis
Carcinoma, Hepatocellular - enzymology
Hepatitis - enzymology
Humans
Immunoelectrophoresis
Isoenzymes - blood
Liver Cirrhosis - enzymology
Liver Neoplasms - diagnosis
Liver Neoplasms - enzymology
Rabbits
Online AccessGet more information

Cover

More Information
Summary:Serum fast band of glycylproline dipeptidyl aminopeptidase isoenzyme (GPDA-F) is useful to the diagnosis of hepatocellular carcinoma, especially for the cases without expression of alpha-fetoprotein (AFP). Polyacrylamide electrophoresis for detection of GPDA-F is relatively complicated and has limitation in its clinical use. This study was to establish a simple and easy method of immunoelectrophoresis to detect serum GPDA-F, and evaluate clinical value of GPDA-F in the diagnosis of hepatocelluar carcinoma. Serum GPDA-F was purified to raise polyclonal GPDA-F antibody, and immunoelectrophoresis was established for the detection of serum GPDA-F. Serum GPDA-F in 99 specimens of hepatocellular carcinoma and 115 specimens of benign liver diseases (36 cases of liver cirrhosis, 23 cases of acute hepatitis, 38 cases of chronic hepatitis, and 18 cases of benign liver space-occupying lesions) was simultaneously detected by both polyacrylamide electrophoresis and immunoelectrophoresis. The clinical value of serum GPDA-F