Optimized conditions for cholinesterase determination

The impact of the buffer capacity and of buffer nature on the effective activity of enzymes was demonstrated by the example of blood choline esterase (CE). The enzyme activity, when determined in vitro, is inversely related with a size of the buffer capacity. The substitution of sodium for potassium...

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Bibliographic Details
Published inKlinicheskaia laboratornaia diagnostika no. 5; p. 35
Main Author Gerasimov, I G
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.05.2004
Subjects
Adult
Cholinesterases - blood
Female
Humans
Hydrogen-Ion Concentration
Male
Potentiometry
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Summary:The impact of the buffer capacity and of buffer nature on the effective activity of enzymes was demonstrated by the example of blood choline esterase (CE). The enzyme activity, when determined in vitro, is inversely related with a size of the buffer capacity. The substitution of sodium for potassium entails a changed activity in the phosphate buffers, e.g. in case of CE--by 30%, which essentially surpasses the influence of pH within a range of 7.0-8.0. A possible mechanism of the observed phenomenon is under consideration.
ISSN:0869-2084