PRODUCTION OF CONJUGATED LINOLEIC ACID BY WHOLE-CELL OFCTOBACILLUS PLANTARUM1F

• Published in: Biotechnology, biotechnological equipment Vol. 25; no. 1
• Main Authors: Zhao, H-W, Lv, J-P, Li, S-R
• Format: Journal Article
• Language: English
• Published: 01.02.2011
• Subjects: Lactobacillus plantarum
• DOI: 10.5504/bbeq.2011.0008
• ISSN: 1310-2818

Whole cell of Lactobacillus plantarum A6-1F was employed as the catalyst to produce CLA (conjugated linoleic acid) from LA (linoleic acid). In order to enhance the activity of the cell enzyme, we introduced the permeabilization technology for modifying cells. Data showed that freeze-thawing was a suitable and economical permeabilization method for the modified cell of Lactobacillus plantarum, which made the yield approximately 20% more than the untreated fresh cell. It was also observed that pH, temperature, substrate concentration, and cell concentration were the main factors that affected the yield of CLA and the reaction conditions of A6-1F were studied. Under the optimal reaction conditions (pH 7.0, 15% (w/v) cell concentration, 1.5 mg/mL substrate concentration, 37oC), the maximum production of CLA (275.7 mu g/mL) was obtained in PBS after 2h reaction. The main CLA isomer in the reaction mixture was c9, t11 CLA that has physiological activity.