Evaluation of recombinant human interferon a-2b structure and stability by in-gel tryptic digestion, H/D exchange and mass spectrometry

• Published in: Journal of pharmaceutical and biomedical analysis Vol. 40; no. 3; pp. 781 - 787
• Main Authors: Cindric, Mario, Galic, Nives, Vuletic, Marko, Klaric, Mia, Drevenkar, Vlasta
• Format: Journal Article
• Language: English
• Published: 01.02.2006

Stability and structure of recombinant interferon a-2b (rHuINF a-2b) was studied by mass spectrometry (MALDI-TOF and Q-TOF MS), chromatography (LC- UV-FLD-DAD, LC-MS) and CD spectroscopy. Besides analysis of the substance according to Ph. Eur. methods, two additional mass spectrometric methods were...