Detection and evaluation of non-recombinants in cDNA libraries by multiple cloning region PCR

• Published in: BioTechniques Vol. 32; no. 1; pp. 88 - 92
• Main Authors: Bandyopadhyay, Dilip, Vesuna, Farhad D
• Format: Technical Report
• Language: English
• Published: 01.01.2002

Bacteriophages that are routinely used in cDNA libraries do not require any biological selection for forming plaques. Thus parental non-recombinant phages are always found in variable proportions together with recombinant ones in all cDNA libraries. The presence of non-recombinants in significant pr...