Real-Time Culture-Independent Microbial Profiling Onboard the International Space Station using Nanopore Sequencing

• Published in: Genes
• Main Authors: Stahl-Rommel, Sarah, Jain, Miten, Nguyen, Hang N, Arnold, Richard G, Aunon-Chancellor, Serena M, Sharp, G Marie, Castro, Christian, John, Kristen K, Juul, Sissel, Turner, Daniel, Stoddart, David, Paten, Benedict, Akeson, Mark, Burton, Aaron S, Castro-Wallace, Sarah L
• Format: Web Resource
• Language: English
• Published: Hampton NASA/Langley Research Center 16.01.2021
• Subjects: Culture; Extreme environments; International Space Station; Low earth orbits; Microorganisms; Onboard; Optimization; Reagents; Real time; Space missions; Space stations

For the past two decades, microbial monitoring of the International Space Station (ISS) has relied on culture-dependent methods that require return to Earth for analysis. This has a number of limitations, with the most significant being bias towards the detection of culturable organisms and the inhe...