Reaction of Desulfovibrio vulgaris Two-Iron Superoxide Reductase with Superoxide: Insight from Stopped-Flow Spectrophotometry

• Published in: Biochemistry (Easton) Vol. 46; no. 40; pp. 11342 - 11351
• Main Authors: Huang, V W, Emerson, J P, Kurtz, DM Jr
• Format: Journal Article
• Language: English
• Published: 09.10.2007
• Subjects: Desulfovibrio vulgaris

Stopped-flow mixing of the Desulfovibrio vulgaris two-iron superoxide reductase (2Fe-SOR) containing the ferrous active site with superoxide generates a dead time intermediate whose absorption spectrum is identical to that of a putative ferric-hydroperoxo intermediate previously observed by pulse ra...