Bacterial Populations Active in Metabolism of C sub(1) Compounds in the Sediment of Lake Washington, a Freshwater Lake

• Published in: Applied and environmental microbiology Vol. 71; no. 11; pp. 6885 - 6899
• Main Authors: Nercessian, Olivier, Noyes, Emma, Kalyuzhnaya, Marina G, Lidstrom, Mary E, Chistoserdova, Ludmila
• Format: Journal Article
• Language: English
• Published: 01.11.2005
• Subjects: Freshwater; Methylobacter; Methylobacterium; Methylomicrobium; Methylomonas; Methylosinus; Proteobacteria; USA, Washington, Seattle, Washington L

Active members of the bacterial community in the sediment of Lake Washington, with special emphasis on C sub(1) utilizers, were identified by employing two complementary culture-independent approaches: reverse transcription of environmental mRNA and 16S rRNA combined with PCR (RT-PCR) and stable-iso...