T sub(H)2 cytokines modulate the IL-9R expression on human neutrophils
Interleukin (IL)-9 is associated with key pathological features of asthma such as airway hyperresponsiveness, bronchoconstriction and mucus production. Inflammatory responses mediated by IL-9 rely on the expression of the IL-9R which has been reported on lung epithelial cells, T lymphocytes and rece...
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Published in | Biochemical and biophysical research communications Vol. 384; no. 2; pp. 167 - 172 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
26.06.2009
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Online Access | Get full text |
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Abstract | Interleukin (IL)-9 is associated with key pathological features of asthma such as airway hyperresponsiveness, bronchoconstriction and mucus production. Inflammatory responses mediated by IL-9 rely on the expression of the IL-9R which has been reported on lung epithelial cells, T lymphocytes and recently on airway granulocyte infiltrates. In this study, we assessed the regulatory and constitutive cell surface expression of the IL-9R alpha in unfractionated and purified human neutrophils from atopic asthmatics, atopic non-asthmatics and healthy normal controls. We demonstrate that T sub(H)2 cytokines (IL-4 or IL-13) and granulocyte macrophage-colony stimulating factor (GM-CSF) up-regulated mRNA and cell surface expression levels of the IL-9R alpha in primary human and HL-60 differentiated neutrophils. Pharmacological inhibition of NF- Kappa B did not affect T sub(H)2-mediated IL-9R alpha expression in human neutrophils although IFN- gamma and IL-10 down-regulated IL-9R alpha expression when co-incubated with IL-4, IL-13 or GM-CSF. Collectively, our results reveal a regulatory function for IFN- gamma and IL-10 on modulating the inducible IL-9R alpha expression levels on peripheral blood neutrophils by T sub(H)2 cytokines. |
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AbstractList | Interleukin (IL)-9 is associated with key pathological features of asthma such as airway hyperresponsiveness, bronchoconstriction and mucus production. Inflammatory responses mediated by IL-9 rely on the expression of the IL-9R which has been reported on lung epithelial cells, T lymphocytes and recently on airway granulocyte infiltrates. In this study, we assessed the regulatory and constitutive cell surface expression of the IL-9R alpha in unfractionated and purified human neutrophils from atopic asthmatics, atopic non-asthmatics and healthy normal controls. We demonstrate that T sub(H)2 cytokines (IL-4 or IL-13) and granulocyte macrophage-colony stimulating factor (GM-CSF) up-regulated mRNA and cell surface expression levels of the IL-9R alpha in primary human and HL-60 differentiated neutrophils. Pharmacological inhibition of NF- Kappa B did not affect T sub(H)2-mediated IL-9R alpha expression in human neutrophils although IFN- gamma and IL-10 down-regulated IL-9R alpha expression when co-incubated with IL-4, IL-13 or GM-CSF. Collectively, our results reveal a regulatory function for IFN- gamma and IL-10 on modulating the inducible IL-9R alpha expression levels on peripheral blood neutrophils by T sub(H)2 cytokines. |
Author | Shan, L Gounni, A S HayGlass, K T Takhar, M K Simons, F E Dragon, S |
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Title | T sub(H)2 cytokines modulate the IL-9R expression on human neutrophils |
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