Synthesis and Characterization of Immunogens Based on Calix[4]arenecrown-6 for the Generation of Antibodies Directed Towards Cesium Ions

With the aim of generating antibodies directed towards cesium, a calix[4]arene-crown-6 in the 1,3-alternate conformation bearing an N-hydroxysuccinimide function was synthesized. The calixarene was coupled to bovine serum albumin using different calixarene/protein ratios, and cesium was loaded. Conj...

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Published inBioconjugate chemistry Vol. 17; no. 5; pp. 1346 - 1350
Main Authors Safi, S, Asfari, Z, Ehret-Sabatier, L, Leroy, M, Hagege, A
Format Journal Article
LanguageEnglish
Published 01.09.2006
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Summary:With the aim of generating antibodies directed towards cesium, a calix[4]arene-crown-6 in the 1,3-alternate conformation bearing an N-hydroxysuccinimide function was synthesized. The calixarene was coupled to bovine serum albumin using different calixarene/protein ratios, and cesium was loaded. Conjugates were characterized using trinitrobenzenesulfonic acid derivatization, matrix assisted laser desorption ionisation-mass spectrometry (MALDI-TOF-MS), and inductively coupled plasma-mass spectrometry (ICP-MS). Targeted lysines were determined after enzymatic cleavage followed by MALDI-TOF-MS, and a progressive targeting related to lysines accessibility was observed.
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ISSN:1043-1802
DOI:10.1021/bc060109h