Key Features Determining the Specificity of Aspartic Proteinase Inhibition by the Helix-forming IA sub(3) Polypeptide

• Published in: The Journal of biological chemistry Vol. 282; no. 9; pp. 6508 - 6516
• Main Authors: Winterburn, Tim J, Wyatt, David M, Phylip, Lowri H, Bur, Daniel, Harrison, Rebecca J, Berry, Colin, Kay, John
• Format: Journal Article
• Language: English
• Published: 02.03.2007
• Subjects: Aspergillus fumigatus; Pichia pastoris; Saccharomyces castellii; Saccharomyces cerevisiae

The 68-residue IA sub(3) polypeptide from Saccharomyces cerevisiae is essentially unstructured. It inhibits its target aspartic proteinase through an unprecedented mechanism whereby residues 2-32 of the polypeptide adopt an amphipathic alpha -helical conformation upon contact with the active site of...