Membrane Potential Fluctuations Resulting From Submembrane Ca super(2+) Releases in Rabbit Sinoatrial Nodal Cells Impart an Exponential Phase to the Late Diastolic Depolarization That Controls Their Chronotropic State
Stochastic but roughly periodic LCRs (Local subsarcolemmal ryanodine receptor-mediated Ca super(2+) Releases) during the late phase of diastolic depolarization (DD) in rabbit sinoatrial nodal pacemaker cells (SANCs) generate an inward current (I sub(NCX)) via the Na super(+)/Ca super(2+) exchanger....
Saved in:
Published in | Circulation research Vol. 99; no. 9; pp. 979 - 987 |
---|---|
Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
01.10.2006
|
Online Access | Get full text |
Cover
Loading…
Abstract | Stochastic but roughly periodic LCRs (Local subsarcolemmal ryanodine receptor-mediated Ca super(2+) Releases) during the late phase of diastolic depolarization (DD) in rabbit sinoatrial nodal pacemaker cells (SANCs) generate an inward current (I sub(NCX)) via the Na super(+)/Ca super(2+) exchanger. Although LCR characteristics have been correlated with spontaneous beating, the specific link between LCR characteristics and SANC spontaneous beating rate, ie, impact of LCRs on the fine structure of the DD, have not been explicitly defined. Here we determined how LCRs and resultant I sub(NCX) impact on the DD fine structure to control the spontaneous SANC firing rate. Membrane potential (V sub(m)) recordings combined with confocal Ca super(2+) measurements showed that LCRs impart a nonlinear, exponentially rising phase to the DD later part, which exhibited beat-to-beat V sub(m) fluctuations with an amplitude of approximately 2 mV. Maneuvers that altered LCR timing or amplitude of the nonlinear DD (ryanodine, BAPTA, nifedipine or isoproterenol) produced corresponding changes in V sub(m) fluctuations during the nonlinear DD component, and the V sub(m) fluctuation response evoked by these maneuvers was tightly correlated with the concurrent changes in spontaneous beating rate induced by these perturbations. Numerical modeling, using measured LCR characteristics under these perturbations, predicted a family of local I sub(NCX) that reproduced V sub(m) fluctuations measured experimentally and determined the onset and amplitude of the nonlinear DD component and the beating rate. Thus, beat-to-beat V sub(m) fluctuations during late DD phase reflect the underlying LCR/I sub(NCX) events, and the ensemble of these events forms the nonlinear DD component that ultimately controls the SANC chronotropic state in tight cooperation with surface membrane ion channels. |
---|---|
AbstractList | Stochastic but roughly periodic LCRs (Local subsarcolemmal ryanodine receptor-mediated Ca super(2+) Releases) during the late phase of diastolic depolarization (DD) in rabbit sinoatrial nodal pacemaker cells (SANCs) generate an inward current (I sub(NCX)) via the Na super(+)/Ca super(2+) exchanger. Although LCR characteristics have been correlated with spontaneous beating, the specific link between LCR characteristics and SANC spontaneous beating rate, ie, impact of LCRs on the fine structure of the DD, have not been explicitly defined. Here we determined how LCRs and resultant I sub(NCX) impact on the DD fine structure to control the spontaneous SANC firing rate. Membrane potential (V sub(m)) recordings combined with confocal Ca super(2+) measurements showed that LCRs impart a nonlinear, exponentially rising phase to the DD later part, which exhibited beat-to-beat V sub(m) fluctuations with an amplitude of approximately 2 mV. Maneuvers that altered LCR timing or amplitude of the nonlinear DD (ryanodine, BAPTA, nifedipine or isoproterenol) produced corresponding changes in V sub(m) fluctuations during the nonlinear DD component, and the V sub(m) fluctuation response evoked by these maneuvers was tightly correlated with the concurrent changes in spontaneous beating rate induced by these perturbations. Numerical modeling, using measured LCR characteristics under these perturbations, predicted a family of local I sub(NCX) that reproduced V sub(m) fluctuations measured experimentally and determined the onset and amplitude of the nonlinear DD component and the beating rate. Thus, beat-to-beat V sub(m) fluctuations during late DD phase reflect the underlying LCR/I sub(NCX) events, and the ensemble of these events forms the nonlinear DD component that ultimately controls the SANC chronotropic state in tight cooperation with surface membrane ion channels. |
Author | Bogdanov, Konstantin Y Spurgeon, Harold A Vinogradova, Tatiana M Maltsev, Victor A Lyashkov, Alexey E Stern, Michael D Lakatta, Edward G |
Author_xml | – sequence: 1 givenname: Konstantin surname: Bogdanov middlename: Y fullname: Bogdanov, Konstantin Y – sequence: 2 givenname: Victor surname: Maltsev middlename: A fullname: Maltsev, Victor A – sequence: 3 givenname: Tatiana surname: Vinogradova middlename: M fullname: Vinogradova, Tatiana M – sequence: 4 givenname: Alexey surname: Lyashkov middlename: E fullname: Lyashkov, Alexey E – sequence: 5 givenname: Harold surname: Spurgeon middlename: A fullname: Spurgeon, Harold A – sequence: 6 givenname: Michael surname: Stern middlename: D fullname: Stern, Michael D – sequence: 7 givenname: Edward surname: Lakatta middlename: G fullname: Lakatta, Edward G |
BookMark | eNqNj91KA0EMhQepYKu-Q65EkcJut7Xu9bZFQaV0e1-yNbojs5N1kgHxTX0bx797bxII55x8Z2QGnj0dmGE-m0zH09k8H5hhlmXleF4U2ZEZibxkWT4tJuXQfNxT1wT0BGtW8mrRwcrFvUZUy15gQxKdWv8Mq8Ad1LHp_hwVgsSewvnk8iLpHKGQgPWwwaaxCrX1jBq-Ih_4Mc2KnBO47XoMCuhh-dYn0p-n6za5QRm0JbhDJVhYFGVn97Cgnh0G-_7NBNsWFSr2GjjFbVuyAao2sOd06ZO-1uQ_MYdP6IROf_exOVstt9XNuA_8Gkl011nZJ6JUhaPs8rKYX10XZfFv4SfTFXds |
ContentType | Journal Article |
DBID | 7QP |
DatabaseName | Calcium & Calcified Tissue Abstracts |
DatabaseTitle | Calcium & Calcified Tissue Abstracts |
DatabaseTitleList | Calcium & Calcified Tissue Abstracts |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Medicine |
EISSN | 1524-4571 |
EndPage | 987 |
GroupedDBID | --- -~X .-D .3C .55 .GJ .Z2 01R 0R~ 18M 1J1 29B 2WC 40H 4Q1 4Q2 4Q3 53G 5GY 5RE 5VS 71W 77Y 7O~ 7QP AAAAV AAAXR AAGIX AAHPQ AAIQE AAMOA AAMTA AARTV AASOK AAXQO ABBUW ABDIG ABJNI ABOCM ABQRW ABXVJ ABZAD ACCJW ACDDN ACEWG ACGFO ACGFS ACILI ACNWC ACPRK ACWDW ACWRI ACXNZ ADBBV ADGGA ADHPY ADNKB AE3 AE6 AEETU AENEX AFDTB AFFNX AFUWQ AGINI AHMBA AHOMT AHQNM AHRYX AHVBC AIJEX AINUH AJIOK AJNWD AJNYG AJZMW ALMA_UNASSIGNED_HOLDINGS AMJPA AMNEI AWKKM BAWUL BOYCO BQLVK C1A C45 CS3 DIK DIWNM DU5 DUNZO E.X E3Z EBS EJD EX3 F2K F2L F2M F2N F5P FCALG FL- FRP FW0 GX1 H0~ HZ~ H~9 IKREB IKYAY IN~ J5H JK3 JK8 K8S KD2 KMI KQ8 L-C L7B N9A N~7 N~B O9- OAG OAH OB2 OCUKA ODA OK1 OL1 OLG OLH OLU OLV OLW OLY OLZ OPUJH ORVUJ OUVQU OVD OVDNE OVIDH OVLEI OWW OWY OXXIT P2P PQQKQ RAH RHF RLZ S4R S4S T8P TEORI TR2 UPT V2I VVN W3M W8F WH7 WOQ WOW X3V X3W X7M YFH YOC ZFV ZZMQN |
ID | FETCH-proquest_miscellaneous_193768393 |
ISSN | 0009-7330 |
IngestDate | Thu Aug 15 22:24:58 EDT 2024 |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 9 |
Language | English |
LinkModel | OpenURL |
MergedId | FETCHMERGED-proquest_miscellaneous_193768393 |
Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 content type line 23 ObjectType-Feature-2 |
PQID | 19376839 |
PQPubID | 23462 |
ParticipantIDs | proquest_miscellaneous_19376839 |
PublicationCentury | 2000 |
PublicationDate | 20061001 |
PublicationDateYYYYMMDD | 2006-10-01 |
PublicationDate_xml | – month: 10 year: 2006 text: 20061001 day: 01 |
PublicationDecade | 2000 |
PublicationTitle | Circulation research |
PublicationYear | 2006 |
SSID | ssj0014329 |
Score | 3.7184079 |
Snippet | Stochastic but roughly periodic LCRs (Local subsarcolemmal ryanodine receptor-mediated Ca super(2+) Releases) during the late phase of diastolic depolarization... |
SourceID | proquest |
SourceType | Aggregation Database |
StartPage | 979 |
Title | Membrane Potential Fluctuations Resulting From Submembrane Ca super(2+) Releases in Rabbit Sinoatrial Nodal Cells Impart an Exponential Phase to the Late Diastolic Depolarization That Controls Their Chronotropic State |
URI | https://search.proquest.com/docview/19376839 |
Volume | 99 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnZ1Lj9MwEMet7h4QF8RTsLzmAAgUBdEkbZIjW1qtoC1LyaLeKufRNqKNqyZZAZ-TC9-GGdt5LCAtcIkqK3GdzE_j_9hjm7En_ZC7rpdgWNLH2MTp81dmaHctM_Zizw9D343kPgWTaf_kzHk77807ne-trKWyCF9G3_64ruR_rIplaFdaJfsPlq0rxQL8jfbFK1oYr39l40myxWAXZeKpKCjrBz_3aFPSkhCV3zZLcsoXzFaoT8WWnMS2emLAjbzckb0866l1TIMDM-yBsE-TCbIzHoZpYXxMM8HVwR5TEdM4Q7LZ5LSlMLbGQNcw_LITmf7r0zU-XWnZMWpY9KcctSVto_0GdT7G0HrRpxGseUGLDSlLPqesj3RvyG16BZbs8H6pgdvCeZDuI33SmKE3KKoHso_FKuaZOJdOS-ldfGmjFukTvilQAMiU3pQmKZoB3E_4gqs9j8W5FNEBJ4fHmyHi8Veerz-rqmktEHrQ4S8DJVXKXeP8fdO19TRQov295ZhOT50CU3UI6sQmDb7f8u6-OvdGCwVfK4ULe3hP3y9GZ-PxIhjOgwN2YHcpzfTdh2Zmy7EtvzrdjxrzmwaQwia4zq7piAReK7xusE6S3WRXJjrn4hb7UVEGNWXQpgxqyoAogxZlMOAgKXtuGS-g4gvSDBRf0PAFki-QfIHiC3gGLb5A8gWFAOQLiC-o-YKLfAHxBRVfIPmCNl8g-brNno2GweDErD7NAp0ezWRh00WZLzDqwDDZ9u077DDDZtxl4GHs0XMiOQ3sLJNl2LW73HEdmvdeWlF8jz2-pLKjS--4z642YD1gh8W-TB6iJi3CR9LIPwEwBqQJ |
link.rule.ids | 315,786,790 |
linkProvider | Colorado Alliance of Research Libraries |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Membrane+Potential+Fluctuations+Resulting+From+Submembrane+Ca+super%282%2B%29+Releases+in+Rabbit+Sinoatrial+Nodal+Cells+Impart+an+Exponential+Phase+to+the+Late+Diastolic+Depolarization+That+Controls+Their+Chronotropic+State&rft.jtitle=Circulation+research&rft.au=Bogdanov%2C+Konstantin+Y&rft.au=Maltsev%2C+Victor+A&rft.au=Vinogradova%2C+Tatiana+M&rft.au=Lyashkov%2C+Alexey+E&rft.date=2006-10-01&rft.issn=0009-7330&rft.eissn=1524-4571&rft.volume=99&rft.issue=9&rft.spage=979&rft.epage=987&rft.externalDBID=NO_FULL_TEXT |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0009-7330&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0009-7330&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0009-7330&client=summon |