Fusarium population in asparagus fields in relation with cultural practices, geographical localization and age of the plant
Asparagus officinalis is a valuable perennial crop that showed a decline in the past decades leading to a loss in productivity, longevity and profitability of the fields. Fusarium species are the main pathogens responsible for the asparagus fields productivity decline and we previously showed that t...
Saved in:
Published in | Phytopathology Vol. 94; no. 6 |
---|---|
Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
01.06.2004
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | Asparagus officinalis is a valuable perennial crop that showed a decline in the past decades leading to a loss in productivity, longevity and profitability of the fields. Fusarium species are the main pathogens responsible for the asparagus fields productivity decline and we previously showed that their populations in asparagus fields were composed of numerous species. In order to be able to rapidly process large number of samples, a Fusarium specific PCR-DGGE (denaturing gradient gel electrophoresis) method was developed and applied. DGGE is a highly discriminant culture-independent method with a theoretical resolution limit of one base pair between PCR products of similar length. Fusarium species diversity in commercial production fields is analysed by both PCR-DGGE and isolation on MBA selective medium, in relation with the following factors: geographic production area, effect of the first cropping season, cultural practices, arbuscular mycorrhizal fungi diversity, and age of the asparagus fields (1st year: installation of plants in the field; 3rd year: beginning of the harvest; and 5-6th years: first observed decline of production). |
---|---|
AbstractList | Asparagus officinalis is a valuable perennial crop that showed a decline in the past decades leading to a loss in productivity, longevity and profitability of the fields. Fusarium species are the main pathogens responsible for the asparagus fields productivity decline and we previously showed that their populations in asparagus fields were composed of numerous species. In order to be able to rapidly process large number of samples, a Fusarium specific PCR-DGGE (denaturing gradient gel electrophoresis) method was developed and applied. DGGE is a highly discriminant culture-independent method with a theoretical resolution limit of one base pair between PCR products of similar length. Fusarium species diversity in commercial production fields is analysed by both PCR-DGGE and isolation on MBA selective medium, in relation with the following factors: geographic production area, effect of the first cropping season, cultural practices, arbuscular mycorrhizal fungi diversity, and age of the asparagus fields (1st year: installation of plants in the field; 3rd year: beginning of the harvest; and 5-6th years: first observed decline of production). |
Author | St-Arnaud, M Vujanovic, V Yergeau, E |
Author_xml | – sequence: 1 givenname: E surname: Yergeau fullname: Yergeau, E – sequence: 2 givenname: V surname: Vujanovic fullname: Vujanovic, V – sequence: 3 givenname: M surname: St-Arnaud fullname: St-Arnaud, M |
BookMark | eNqNi0FuwjAQRb0AqYRyh1mxKpJDmpasUSMO0AU7NHImySBjux5bSPTyDSoHYPO_9P77hZo572imFlpX5aZ5b44vqhA5a60_d_XHQv22WTByvkDwIVtM7B2wA5SAEYcs0DPZTu4s0mO_chrBZJtyRAshoklsSN5gID9EDCObiVs_Jd_-L-g6wIHA95BGgmDRpVc179EKrR69VOv263t_2ITofzJJOl1YDNnJJZ_lVO50rZttWT0t_gGB_1RP |
ContentType | Journal Article |
DBID | M7N |
DatabaseName | Algology Mycology and Protozoology Abstracts (Microbiology C) |
DatabaseTitle | Algology Mycology and Protozoology Abstracts (Microbiology C) |
DatabaseTitleList | Algology Mycology and Protozoology Abstracts (Microbiology C) |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Botany Agriculture |
GroupedDBID | --- -DZ -~X 123 29O 2WC 3V. 53G 7X2 7X7 88A 88E 8AO 8CJ 8FE 8FH 8FI 8FJ 8FW 8R4 8R5 AAHBH AAYJJ ABOGM ABPPZ ABUWG ACBNA ACBTR ACNCT ACPRK ADBBV ADNWM AENEX AFKRA AFRAH ALIPV ALMA_UNASSIGNED_HOLDINGS ATCPS BAWUL BBNVY BENPR BHPHI BKSAR BPHCQ BVXVI C1A CCPQU CS3 D1J DIK EBS EJD F5P FRP FYUFA HCIFZ HMCUK HYO K-O L7B LK8 M0K M0L M1P M7N M7P NEJ NHB OK1 PCBAR PQQKQ PROAC PSQYO Q2X RPS S0X TR2 TWZ UKHRP UKR VQA WH7 XOL YCJ ~KM |
ID | FETCH-proquest_miscellaneous_180509213 |
ISSN | 0031-949X |
IngestDate | Fri Aug 16 05:28:15 EDT 2024 |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 6 |
Language | English |
LinkModel | OpenURL |
MergedId | FETCHMERGED-proquest_miscellaneous_180509213 |
Notes | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Conference-1 ObjectType-Feature-3 content type line 23 SourceType-Conference Papers & Proceedings-2 |
PQID | 18050921 |
PQPubID | 23462 |
ParticipantIDs | proquest_miscellaneous_18050921 |
PublicationCentury | 2000 |
PublicationDate | 20040601 |
PublicationDateYYYYMMDD | 2004-06-01 |
PublicationDate_xml | – month: 06 year: 2004 text: 20040601 day: 01 |
PublicationDecade | 2000 |
PublicationTitle | Phytopathology |
PublicationYear | 2004 |
SSID | ssj0007856 |
Score | 3.484926 |
Snippet | Asparagus officinalis is a valuable perennial crop that showed a decline in the past decades leading to a loss in productivity, longevity and profitability of... |
SourceID | proquest |
SourceType | Aggregation Database |
SubjectTerms | Asparagus officinalis Fusarium |
Title | Fusarium population in asparagus fields in relation with cultural practices, geographical localization and age of the plant |
URI | https://search.proquest.com/docview/18050921 |
Volume | 94 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnZ3PT8IwFMcbQQ96MIoaf2IPxgvOODYHHMFAiCFIdJh5WrqfaHQjbD2g_7yv3daxSIJ6aZZmadJ89trX7n3fQ-gC7KupMHEMUWVbUhuaLbU8ry4RckMsT3M8x-bZPodaf6zeG7dGXk-Tq0ti69r-XKor-Q9V6AOuTCX7B7JiUOiAZ-ALLRCG9leMezSCoy79qE1FGS52f0EiltDbp1GNx6dFuWYlDNJY8yzfRiaS4jT9pCD6hHPjm1wq0kwyuvoioGD6ToLCnf5oMo9DVty4cEn_wpSdhBb0Ds_0jQQhLE-FANunWGrDdOlChePsJkLNI6ay1VWRpZbKS-OK1TUpYZx-RUuSXg8fzN54MDD1rqGXUEmRWVxmwxCnZnBheAleMfiPTZN7AvoO2k5deNxOeOyiNTeooK22P0vTmLgVtNEJweme76GvDBHOEeHXAAtEOEHE-jJEmCHCGSIsEF3hRUB4ERAGQBgA4dDDAAhzQPvostfV7_pSNhETbJr9qCGBG9LIlJssK09dVg5QOQgD9xBhcCI0cP7qrioTltEHTIs4qgf2BB6s5ahH6HzFYMcr3zhBmznWU1SOZ9Q9A5crtqocSRWtd7rD0eM3GuVBGg |
link.rule.ids | 315,786,790,31755,33780 |
linkProvider | ProQuest |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Fusarium+population+in+asparagus+fields+in+relation+with+cultural+practices%2C+geographical+localization+and+age+of+the+plant&rft.jtitle=Phytopathology&rft.au=Yergeau%2C+E&rft.au=Vujanovic%2C+V&rft.au=St-Arnaud%2C+M&rft.date=2004-06-01&rft.issn=0031-949X&rft.volume=94&rft.issue=6&rft.externalDBID=NO_FULL_TEXT |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0031-949X&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0031-949X&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0031-949X&client=summon |