Sensitive Determination of Panaxadiol Using Rhodamine B as Sensitizing Derivatization-Reagent by Ultrahigh Performance Liquid Chromatography-Triple Quadrupole Mass Spectrometry

• Published in: Fēnxī huàxué Vol. 42; no. 11; pp. 1629 - 1633
• Main Authors: ZHAO, Xian-En, LU, Tao, WEI, Na, ZHU, Shu-Yun, YOU, Jin-Mao
• Format: Journal Article
• Language: English
• Published: 01.11.2014
• Subjects: Cosmetics; Derivatives; Ethyl alcohol; Linearity; Mass spectrometry; Quadrupoles; Rhodamine; Solvents
• ISSN: 0253-3820
• DOI: 10.11895/j.issn.0253-3820.140540

In virtue of the strategy of ultrasonic-assisted dispersive liquid-liquid microextraction (UA-DLLME) combined with Rhodamine B derivatization, a new ultrahigh performance liquid chromatography - triple quadrupole mass spectrometry (UHPLC-MS/MS) method was devepled for the sensitive determination of Panaxadiol (PD). UA-DLLME and derivatization conditions were investigated. PD in cosmetics and ginseng was extracted by UA-DLLME (150 mu L of chloroform as extraction solvent and 500 of mu L ethanol as disperser solvent, 3 min). Using Rhodamine B as precolumn derivatization reagent, the stable derivative of PD was obtained in acetonitrile at 70 [degrees]C for 30 min under microwave radiation (800 W) with the catalyst of N,N'-carbonyldiimidazole (GDI) and 4-dimethylaminopyridine (DMAP). And then the PD derivative was separated and detected within 3 min by multiple reaction monitoring (MRM) mode of UHPLC-MS/MS. LOD was 4.0 ng/L, and the LOQ was 15.0 ng/L. This method had good linearity, precision and recovery, and showed obvious advantages such as sensitivity and low matrix effect comparing to the previously reported methods.