Ammonium addition inhibits super(13)C-methane incorporation into methanotroph membrane lipids in a freshwater sediment
To investigate the effect of ammonium addition on the species composition and activity of freshwater methane oxidizing bacteria, intact sediment cores were labeled with super(13)CH sub(4) and incubated under ambient and elevated ammonium concentrations. After 7 days, methanotroph activity was assess...
Saved in:
Published in | FEMS microbiology ecology Vol. 29; no. 1; pp. 81 - 89 |
---|---|
Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
01.05.1999
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | To investigate the effect of ammonium addition on the species composition and activity of freshwater methane oxidizing bacteria, intact sediment cores were labeled with super(13)CH sub(4) and incubated under ambient and elevated ammonium concentrations. After 7 days, methanotroph activity was assessed by quantifying the isotopic composition of the carbon in membrane lipids. The 16-carbon rather than the 18-carbon methanotroph-specific biomarkers showed a clear enrichment in super(13)C, suggesting the importance of group I methanotrophs in these sediments. Ammonium addition resulted in a depleted isotopic signal compared to ambient controls, suggesting that high ammonium concentrations inhibit methane incorporation into cellular components. These results compare favorably with studies that showed ammonium inhibition of methane oxidation, and extend these findings by demonstrating the effect of nitrogen fertilization on methanotroph lipid synthesis. |
---|---|
AbstractList | To investigate the effect of ammonium addition on the species composition and activity of freshwater methane oxidizing bacteria, intact sediment cores were labeled with super(13)CH sub(4) and incubated under ambient and elevated ammonium concentrations. After 7 days, methanotroph activity was assessed by quantifying the isotopic composition of the carbon in membrane lipids. The 16-carbon rather than the 18-carbon methanotroph-specific biomarkers showed a clear enrichment in super(13)C, suggesting the importance of group I methanotrophs in these sediments. Ammonium addition resulted in a depleted isotopic signal compared to ambient controls, suggesting that high ammonium concentrations inhibit methane incorporation into cellular components. These results compare favorably with studies that showed ammonium inhibition of methane oxidation, and extend these findings by demonstrating the effect of nitrogen fertilization on methanotroph lipid synthesis. |
Author | Laanbroek, HJ Boschker, HTS Pel, R Nold, S C |
Author_xml | – sequence: 1 givenname: S surname: Nold middlename: C fullname: Nold, S C – sequence: 2 givenname: HTS surname: Boschker fullname: Boschker, HTS – sequence: 3 givenname: R surname: Pel fullname: Pel, R – sequence: 4 givenname: HJ surname: Laanbroek fullname: Laanbroek, HJ |
BookMark | eNqNjD1vAjEMhjOAxOdPQMpUwXA0udCjNyLUip3uKHBG5-oShzjX_n1SFXWuB1vv-zzyRAw8eRBiodVaK109H_N6LapNXS3reqXylIUeiPFfPRIT5k-l9IvZqLH42jlHHnsnbdNgQvISfYtnTCy5DxCX2qz2hYPUWg-ZXSgGivZhJpK_iFKk0ObgzvFH7DBgw9mQVl4jcPttE0TJ0KADn2ZieLUdw_xxp-Lp_e1jfyhCpFsPnE4O-QJdl39Rzye9NVWpTWn-Ld4BkLNYGQ |
ContentType | Journal Article |
DBID | 7SN 7T7 8FD C1K F1W FR3 H95 L.G P64 |
DOI | 10.1016/S0168-6496(99)00002-1 |
DatabaseName | Ecology Abstracts Industrial and Applied Microbiology Abstracts (Microbiology A) Technology Research Database Environmental Sciences and Pollution Management ASFA: Aquatic Sciences and Fisheries Abstracts Engineering Research Database Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources Aquatic Science & Fisheries Abstracts (ASFA) Professional Biotechnology and BioEngineering Abstracts |
DatabaseTitle | Aquatic Science & Fisheries Abstracts (ASFA) Professional Technology Research Database ASFA: Aquatic Sciences and Fisheries Abstracts Engineering Research Database Ecology Abstracts Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources Industrial and Applied Microbiology Abstracts (Microbiology A) Biotechnology and BioEngineering Abstracts Environmental Sciences and Pollution Management |
DatabaseTitleList | Aquatic Science & Fisheries Abstracts (ASFA) Professional |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Biology Environmental Sciences |
EndPage | 89 |
GroupedDBID | --- --K .3N .GA .Y3 05W 0R~ 10A 1B1 1OC 1TH 1~5 31~ 36B 4.4 48X 4G. 50Y 50Z 51W 51X 52M 52N 52O 52P 52S 52T 52W 52X 53G 5GY 5HH 5LA 5VS 66C 7-5 702 7PT 7SN 7T7 7X7 7XC 8-0 8-1 8-3 8-4 8-5 88E 8CJ 8FD 8FE 8FH 8FI 8FJ 8UM 930 A03 AACTN AAEDT AAHBH AAHHS AAIMJ AALRI AAMDB AAMVS AAOGV AAONW AAPQZ AAPXW AAQFI AAQXK AARHZ AASNB AAUQX AAVAP AAXUO ABCQN ABEUO ABIXL ABMAC ABPTD ABQLI ABUWG ABXVV ACCFJ ACFRR ACGFO ACIUM ACIWK ACPRK ACUFI ACXQS ADBBV ADEZT ADGZP ADHKW ADHZD ADMUD ADPDF ADQBN ADRIX ADRTK ADVEK ADYVW AEEZP AEGPL AEJOX AEKSI AELWJ AEMDU AENEX AENZO AEPUE AEQDE AETBJ AEWNT AFBPY AFFZL AFGWE AFIYH AFKRA AFOFC AFRAH AFXEN AFZJQ AGINJ AGSYK AHHHB AHMBA AI. AIWBW AJAOE AJBDE AJEEA AKRWK AKWXX ALIPV ALMA_UNASSIGNED_HOLDINGS ALUQC APIBT APWMN ARIXL ATCPS AVWKF AXUDD AYOIW AZBYB BAFTC BAYMD BBNVY BCRHZ BDRZF BENPR BEYMZ BHONS BHPHI BPHCQ BQDIO BSWAC BVXVI BY8 C1K CAG CCPQU CDBKE COF CS3 D-E D-F D1J DAKXR DC6 DCZOG DILTD DR2 DU5 EBS EJD EMB F00 F01 F04 F1W F5P FDB FGOYB FHSFR FLUFQ FOEOM FR3 FYUFA G-S G.N GAUVT GJXCC GODZA H.T H.X H95 HAR HCIFZ HMCUK HOLLA HZI HZ~ IHE IX1 J0M J21 K48 KAQDR KBUDW KOP KSI KSN L.G LC2 LC3 LH4 LK8 LP6 LP7 LW6 M1P M41 M7P MK4 N04 N05 N9A NF~ NLBLG NOMLY NQ- NVLIB O9- OAWHX ODMLO OIG OJQWA OK1 OVD OVEED P2P P2X P4D P64 PAFKI PATMY PEELM PQQKQ PSQYO PYCSY Q.N Q11 Q5Y QB0 R.K R2- RIG ROL ROX ROZ RPZ RUSNO RX1 RXO SSZ SUPJJ TEORI TLC TOX UB1 UKHRP V8K VH1 W8V W99 WH7 WQJ WRC WYUIH XG1 YAYTL YKOAZ YXANX ZCN ~02 ~IA ~KM ~WT |
ID | FETCH-proquest_miscellaneous_173621323 |
ISSN | 0168-6496 |
IngestDate | Sat Oct 05 04:21:50 EDT 2024 |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 1 |
Language | English |
LinkModel | OpenURL |
MergedId | FETCHMERGED-proquest_miscellaneous_173621323 |
Notes | ObjectType-Article-2 SourceType-Scholarly Journals-1 content type line 23 ObjectType-Feature-1 |
PQID | 17362132 |
PQPubID | 23462 |
ParticipantIDs | proquest_miscellaneous_17362132 |
PublicationCentury | 1900 |
PublicationDate | 19990501 |
PublicationDateYYYYMMDD | 1999-05-01 |
PublicationDate_xml | – month: 05 year: 1999 text: 19990501 day: 01 |
PublicationDecade | 1990 |
PublicationTitle | FEMS microbiology ecology |
PublicationYear | 1999 |
SSID | ssj0015340 |
Score | 3.196891 |
Snippet | To investigate the effect of ammonium addition on the species composition and activity of freshwater methane oxidizing bacteria, intact sediment cores were... |
SourceID | proquest |
SourceType | Aggregation Database |
StartPage | 81 |
SubjectTerms | ammonium Freshwater methane oxidation |
Title | Ammonium addition inhibits super(13)C-methane incorporation into methanotroph membrane lipids in a freshwater sediment |
URI | https://search.proquest.com/docview/17362132 |
Volume | 29 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1ZS8NAEF60IvginnjWfVBRJJqjSbqPrUSKtFVshLyVzUUDbVN6KPrrnc1msy0WPF5Cs53sks7X2ZnZORA6p2Zkq6FpKaDbqwoTeAqhoaUYgU6JGZEqUVlycqttNV4rj57pybCiLLtk6t8Gn0vzSv7DVRgDvrIs2T9wtpgUBuAz8BeuwGG4_orHNbZUwsKLwzDJoxZ7ic_OAiazEWNGVWOe1HuFNYqmWYGQIC9dzKlB8-RfpdNxOurBzQDMZyDsJ6OEx8rSmxhM8t47ZeUUJ7DXFbEyorun0-rcDJK5ik5RsOCsb-edqTvSJVtPwarOAzoabkcK6P5CEGOT0qE_TqNMZDcepYuClzUQAYHCa2lVFavCW9cKsZs7OubhxWUob-EidmOyVM5zl0OnmPiC2Y3we6qZhNfk5iYO9NtP3YfXZrPrOp67itZ0m5gsANR98oozJ9PI0meLOWW-151c6IqQ63yRb7t3ppK4W2gztyVwjQNjG61Ewx20zruLfuygfUcmMQJZLsUnu-hN4AYL3GCBG5zh5kozrgvM4AXMYIYZPI8ZLDCDOWaAAlMsMYMFZvbQ5YPj3jcU8T5dkDHs4AieTWeTrmaDmqMZurGPSsN0GB0gHAegCdqxr9s0rsRhTElFD9TQ1-IY_uKRfYjOfpjs6EeKY7Qh0XSCStPxLDoFFXDql9Gq7dlltFZ32s8v5YyPX0XkaHw |
link.rule.ids | 315,786,790,27955,27956,31753,33778 |
linkProvider | Oxford University Press |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Ammonium+addition+inhibits+super%2813%29C-methane+incorporation+into+methanotroph+membrane+lipids+in+a+freshwater+sediment&rft.jtitle=FEMS+microbiology+ecology&rft.au=Nold%2C+S+C&rft.au=Boschker%2C+HTS&rft.au=Pel%2C+R&rft.au=Laanbroek%2C+HJ&rft.date=1999-05-01&rft.issn=0168-6496&rft.volume=29&rft.issue=1&rft.spage=81&rft.epage=89&rft_id=info:doi/10.1016%2FS0168-6496%2899%2900002-1&rft.externalDBID=NO_FULL_TEXT |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0168-6496&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0168-6496&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0168-6496&client=summon |