DnaK-dependent ribosome biogenesis in Escherichia coli: competition for dominance between the alleles dnaK756 and dnaK super(+)
The Escherichia coli chaperone DnaK is vital for many cellular functions, including ribosome biogenesis at high temperature. Thus, the dnaK756-ts ( super(R)) mutant, at the non-permissive temperature, is inhibited at a late stage of ribosome assembly, yielding 21S, 32S and 45S precursor particles. T...
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Published in | Molecular & general genetics Vol. 260; no. 2-3; pp. 199 - 206 |
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Main Authors | , |
Format | Journal Article |
Language | English |
Published |
28.11.1998
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Subjects | |
Online Access | Get full text |
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Abstract | The Escherichia coli chaperone DnaK is vital for many cellular functions, including ribosome biogenesis at high temperature. Thus, the dnaK756-ts ( super(R)) mutant, at the non-permissive temperature, is inhibited at a late stage of ribosome assembly, yielding 21S, 32S and 45S precursor particles. This defect, unlike the resistance and thermosensitivity phenotypes, is not complemented by lysogenisation with a transducing phage dnaK super(+) bearing the wild-type dnaK gene. However this dominant phenotype becomes recessive when dnaK super(+) is expressed from a medium-copy-number plasmid. On the other hand, an excess of DnaK causes an unexpected dominant-lethal effect of the dnaK756 allele near non-permissive temperatures. This interplay between the dnaK super(+) and dnaK756 alleles supports the idea of that DnaK oligomers form in the cell. |
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AbstractList | The Escherichia coli chaperone DnaK is vital for many cellular functions, including ribosome biogenesis at high temperature. Thus, the dnaK756-ts ( super(R)) mutant, at the non-permissive temperature, is inhibited at a late stage of ribosome assembly, yielding 21S, 32S and 45S precursor particles. This defect, unlike the resistance and thermosensitivity phenotypes, is not complemented by lysogenisation with a transducing phage dnaK super(+) bearing the wild-type dnaK gene. However this dominant phenotype becomes recessive when dnaK super(+) is expressed from a medium-copy-number plasmid. On the other hand, an excess of DnaK causes an unexpected dominant-lethal effect of the dnaK756 allele near non-permissive temperatures. This interplay between the dnaK super(+) and dnaK756 alleles supports the idea of that DnaK oligomers form in the cell. |
Author | Sbai, M Alix, J-H |
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Title | DnaK-dependent ribosome biogenesis in Escherichia coli: competition for dominance between the alleles dnaK756 and dnaK super(+) |
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