Deletional mapping of fifteen mouse V sub(H) gene families reveals a common organization for three Igh haplotypes

• Published in: The Journal of immunology (1950) Vol. 156; no. 3; pp. 1038 - 1046
• Main Authors: Mainville, CA, Sheehan, K M, Klaman, L D, Giorgetti, CA, Press, J L, Brodeur, PH
• Format: Journal Article
• Language: English
• Published: 01.01.1996
• ISSN: 0022-1767

In addition to the content of germ-line variable gene segments, the organization of V genes has been implicated in the development of the Ab repertoire. We have searched the expressed V sub(H) genes of BALB/c mice for additional V sub(H) gene families and utilized deletion mapping to explore the extent of V sub(H) gene family interspersion. We have identified and characterized one new V sub(H) gene family (V sub(H)15) and extended our previous studies of the Igh super(a) and Igh super(b) haplotypes to include a third haplotype (Igh super(j)) using a newly developed panel of pre-B cell lines (CXCB cell lines). We conclude that the Igh super(a), Igh super(b), and Igh super(j) haplotypes have a similar Igh-V locus structure. A refined deletional map for 15 V sub(H) gene families and an individual member of the V sub(H)SM7 family (H10) has been constructed based on the deletion profiles of 72 rearranged heavy chain loci. These results demonstrate previously unrecognized examples of interspersion among members of the V sub(H)S107, V sub(H)10, and V sub(H)SM7 families.