Infrared Spectroscopy During Electrocatalytic Turnover Reveals the Ni-L Active Site State During H sub(2) Oxidation by a NiFe Hydrogenase

• Published in: Angewandte Chemie International Edition Vol. 54; no. 24; pp. 7110 - 7113
• Main Authors: Hidalgo, Ricardo, Ash, Philip A, Healy, Adam J, Vincent, Kylie A
• Format: Journal Article
• Language: English
• Published: 01.06.2015
• Subjects: Catalysis; Catalysts; Hydrogenase; Intermetallics; Iron compounds; Nickel; Nickel base alloys; Nickel compounds; Spectra

A novel in situ IR spectroscopic approach is demonstrated for the characterization of hydrogenase during catalytic turnover. E. coli hydrogenase1 (Hyd-1) is adsorbed on a high surface-area carbon electrode and subjected to the same electrochemical control and efficient supply of substrate as in prot...