Ca super(2+) ionophore and phorbol ester stimulate diacylglycerol formation and phosphatidylcholine hydrolysis in rat parotid acinar cells

We investigated the effects of A23187 and phorbol 12,13-dibutyrate (PDBu) on sn-1,2-diacylglycerol (DAG) accumulation and phosphatidylcholine (PC) hydrolysis in rat parotid acinar cells. Both A23187 and PDBu, in concentration ranges of 0.001-0.1 mu M, stimulated DAG accumulation and PC hydrolysis in...

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Published inJapanese journal of pharmacology Vol. 5; no. 1; pp. 97 - 103
Main Authors Komabayashi, T, Yakata, A, Izawa, T, Noguchi, M, Suda, K, Tshuboi, M
Format Journal Article
LanguageEnglish
Published 01.01.1992
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Abstract We investigated the effects of A23187 and phorbol 12,13-dibutyrate (PDBu) on sn-1,2-diacylglycerol (DAG) accumulation and phosphatidylcholine (PC) hydrolysis in rat parotid acinar cells. Both A23187 and PDBu, in concentration ranges of 0.001-0.1 mu M, stimulated DAG accumulation and PC hydrolysis in a time- and concentration-dependent manner. Treatment with A23187 and PDBu stimulated the release of ( super(3)H)choline and ( super(3)H)phosphocholine into the medium, indicating ( super(3)H)PC hydrolysis is due to the activation of phospholipases C and D; however, ( super(3)H)-phosphatidylethanolamine hydrolysis was not indicated. The results suggest that DAG accumulation induced by A23187 and PDBu is related to the PC hydrolysis mediated via the activation of phospholipases C and D, and that it is not related to phosphatidylethanolamine hydrolysis.
AbstractList We investigated the effects of A23187 and phorbol 12,13-dibutyrate (PDBu) on sn-1,2-diacylglycerol (DAG) accumulation and phosphatidylcholine (PC) hydrolysis in rat parotid acinar cells. Both A23187 and PDBu, in concentration ranges of 0.001-0.1 mu M, stimulated DAG accumulation and PC hydrolysis in a time- and concentration-dependent manner. Treatment with A23187 and PDBu stimulated the release of ( super(3)H)choline and ( super(3)H)phosphocholine into the medium, indicating ( super(3)H)PC hydrolysis is due to the activation of phospholipases C and D; however, ( super(3)H)-phosphatidylethanolamine hydrolysis was not indicated. The results suggest that DAG accumulation induced by A23187 and PDBu is related to the PC hydrolysis mediated via the activation of phospholipases C and D, and that it is not related to phosphatidylethanolamine hydrolysis.
Author Suda, K
Tshuboi, M
Komabayashi, T
Izawa, T
Noguchi, M
Yakata, A
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