Characterization of a glycophospholipid intermediate in the biosynthesis of glyco-phosphatidylinositol anchors accumulating in the Thy-1-negative lymphoma line SIA-b

• Published in: The Journal of biological chemistry Vol. 266; no. 31; pp. 21051 - 21059
• Main Authors: Puoti, A, Desponds, C, Fankhauser, C, Conzelmann, A
• Format: Journal Article
• Language: English
• Published: 01.01.1991
• Subjects: cell membranes; glycophosphatidylinositol

Several mammalian mutant cell lines are deficient in the biosynthesis of glyco-phosphatidylinositol anchors for membrane proteins. When metabolically labeled with ( super(3)H)myo-inositol or ( super(3)H)mannose, two out of five mutant lines (SIA-b and EL4-f) accumulated abnormal lipids which remaine...