Mutational analysis of the human HSP70 protein: Distinct domains for nucleolar localization and adenosine triphosphate binding

• Published in: Cell Vol. 109; no. 5; pp. 1947 - 1962
• Main Authors: Milarski, K L, Morimoto, R I
• Format: Journal Article
• Language: English
• Published: 01.01.1989

The human HSP70 gene was modified in vitro using oligonucleotide-directed mutagenesis to add sequences encoding a peptide from the testis-specific form of human lactate dehydrogenase (LDH) to the carboxy terminus of HSP70. A series of deletion mutants within the HSP70 protein coding region were gene...