The pathogenesis-related protein PR-4b from Theobroma cacao presents RNase activity, Ca super(2+) and Mg super(2+) dependent-DNase activity and antifungal action on Moniliophthora perniciosa
Background: The production and accumulation of pathogenesis-related proteins (PR proteins) in plants in response to biotic or abiotic stresses is well known and is considered as a crucial mechanism for plant defense. A pathogenesis-related protein 4 cDNA was identified from a cacao-Moniliophthora pe...
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Published in | BMC plant biology Vol. 14; no. 1; p. 161 |
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Main Authors | , , , , , , , , , |
Format | Journal Article |
Language | English |
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01.01.2014
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Abstract | Background: The production and accumulation of pathogenesis-related proteins (PR proteins) in plants in response to biotic or abiotic stresses is well known and is considered as a crucial mechanism for plant defense. A pathogenesis-related protein 4 cDNA was identified from a cacao-Moniliophthora perniciosa interaction cDNA library and named TcPR-4b. Results: TcPR-4b presents a Barwin domain with six conserved cysteine residues, but lacks the chitin-binding site. Molecular modeling of TcPR-4b confirmed the importance of the cysteine residues to maintain the protein structure, and of several conserved amino acids for the catalytic activity. In the cacao genome, TcPR-4b belonged to a small multigene family organized mainly on chromosome 5. TcPR-4b RT-qPCR analysis in resistant and susceptible cacao plants infected by M. perniciosa showed an increase of expression at 48 hours after infection (hai) in both cacao genotypes. After the initial stage (24-72 hai), the TcPR-4b expression was observed at all times in the resistant genotypes, while in the susceptible one the expression was concentrated at the final stages of infection (45-90 days after infection). The recombinant TcPR-4b protein showed RNase, and bivalent ions dependent-DNase activity, but no chitinase activity. Moreover, TcPR-4b presented antifungal action against M. perniciosa, and the reduction of M. perniciosa survival was related to ROS production in fungal hyphae. Conclusion: To our knowledge, this is the first report of a PR-4 showing simultaneously RNase, DNase and antifungal properties, but no chitinase activity. Moreover, we showed that the antifungal activity of TcPR-4b is directly related to RNase function. In cacao, TcPR-4b nuclease activities may be related to the establishment and maintenance of resistance, and to the PCD mechanism, in resistant and susceptible cacao genotypes, respectively. |
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AbstractList | Background: The production and accumulation of pathogenesis-related proteins (PR proteins) in plants in response to biotic or abiotic stresses is well known and is considered as a crucial mechanism for plant defense. A pathogenesis-related protein 4 cDNA was identified from a cacao-Moniliophthora perniciosa interaction cDNA library and named TcPR-4b. Results: TcPR-4b presents a Barwin domain with six conserved cysteine residues, but lacks the chitin-binding site. Molecular modeling of TcPR-4b confirmed the importance of the cysteine residues to maintain the protein structure, and of several conserved amino acids for the catalytic activity. In the cacao genome, TcPR-4b belonged to a small multigene family organized mainly on chromosome 5. TcPR-4b RT-qPCR analysis in resistant and susceptible cacao plants infected by M. perniciosa showed an increase of expression at 48 hours after infection (hai) in both cacao genotypes. After the initial stage (24-72 hai), the TcPR-4b expression was observed at all times in the resistant genotypes, while in the susceptible one the expression was concentrated at the final stages of infection (45-90 days after infection). The recombinant TcPR-4b protein showed RNase, and bivalent ions dependent-DNase activity, but no chitinase activity. Moreover, TcPR-4b presented antifungal action against M. perniciosa, and the reduction of M. perniciosa survival was related to ROS production in fungal hyphae. Conclusion: To our knowledge, this is the first report of a PR-4 showing simultaneously RNase, DNase and antifungal properties, but no chitinase activity. Moreover, we showed that the antifungal activity of TcPR-4b is directly related to RNase function. In cacao, TcPR-4b nuclease activities may be related to the establishment and maintenance of resistance, and to the PCD mechanism, in resistant and susceptible cacao genotypes, respectively. |
Author | Matos Lima, Eline Silva Andrade, Bruno Oliveira de Sousa, Aurizangela Pereira Menezes, Sara Pirovani, Carlos Priminho Santos Lima Lemos, Livia da Silva Gesteira, Abelmon de Andrade Silva, Edson Mario Micheli, Fabienne Peres Gramacho, Karina |
Author_xml | – sequence: 1 givenname: Sara surname: Pereira Menezes fullname: Pereira Menezes, Sara – sequence: 2 fullname: de Andrade Silva, Edson Mario – sequence: 3 givenname: Eline surname: Matos Lima fullname: Matos Lima, Eline – sequence: 4 givenname: Aurizangela surname: Oliveira de Sousa fullname: Oliveira de Sousa, Aurizangela – sequence: 5 givenname: Bruno surname: Silva Andrade fullname: Silva Andrade, Bruno – sequence: 6 givenname: Livia surname: Santos Lima Lemos fullname: Santos Lima Lemos, Livia – sequence: 7 givenname: Karina surname: Peres Gramacho fullname: Peres Gramacho, Karina – sequence: 8 givenname: Abelmon surname: da Silva Gesteira fullname: da Silva Gesteira, Abelmon – sequence: 9 givenname: Carlos surname: Pirovani middlename: Priminho fullname: Pirovani, Carlos Priminho – sequence: 10 givenname: Fabienne surname: Micheli fullname: Micheli, Fabienne |
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Title | The pathogenesis-related protein PR-4b from Theobroma cacao presents RNase activity, Ca super(2+) and Mg super(2+) dependent-DNase activity and antifungal action on Moniliophthora perniciosa |
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