Purification and characterization of a neutral processing mannosidase from calf liver acting on (Man) sub(9)(GlcNAc) sub(2) oligosaccharides

• Published in: European journal of biochemistry Vol. 157; no. 3; pp. 563 - 570
• Main Authors: Schweden, J, Legler, G, Bause, E
• Format: Journal Article
• Language: English
• Published: 01.01.1986
• Subjects: cattle; mannosidase; microsomes

A processing mannosidase acting on (Man) sub(9)(GlcNAc) sub(2) oligosaccharides, Man sub(9) mannosidase, has been purified 2190-fold from calf liver crude microsomes by a four-step procedure involving (a) differential salt/detergent extraction, (b) affinity chromatography on AH-Sepharose 4B with N-5...