Can Volatile Organic Metabolites Be Used to Simultaneously Assess Microbial and Mite Contamination Level in Cereal Grains and Coffee Beans? e59338
A novel approach based on headspace solid-phase microextraction (HS-SPME) combined with comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry (GCGC-ToFMS) was developed for the simultaneous screening of microbial and mite contamination level in cereals and coffee beans. T...
Saved in:
Published in | PloS one Vol. 8; no. 4 |
---|---|
Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
01.04.2013
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | A novel approach based on headspace solid-phase microextraction (HS-SPME) combined with comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry (GCGC-ToFMS) was developed for the simultaneous screening of microbial and mite contamination level in cereals and coffee beans. The proposed approach emerges as a powerful tool for the rapid assessment of the microbial contamination level (ca. 70 min versus ca. 72 to 120 h for bacteria and fungi, respectively, using conventional plate counts), and mite contamination (ca. 70 min versus ca. 24 h). A full-factorial design was performed for optimization of the SPME experimental parameters. The methodology was applied to three types of rice (rough, brown, and white rice), oat, wheat, and green and roasted coffee beans. Simultaneously, microbiological analysis of the samples (total aerobic microorganisms, moulds, and yeasts) was performed by conventional plate counts. A set of 54 volatile markers was selected among all the compounds detected by GCGC-ToFMS. Principal Component Analysis (PCA) was applied in order to establish a relationship between potential volatile markers and the level of microbial contamination. Methylbenzene, 3-octanone, 2-nonanone, 2-methyl-3-pentanol, 1-octen-3-ol, and 2-hexanone were associated to samples with higher microbial contamination level, especially in rough rice. Moreover, oat exhibited a high GC peak area of 2-hydroxy-6-methylbenzaldehyde, a sexual and alarm pheromone for adult mites, which in the other matrices appeared as a trace component. The number of mites detected in oat grains was correlated to the GC peak area of the pheromone. The HS-SPME/GCGC-ToFMS methodology can be regarded as the basis for the development of a rapid and versatile method that can be applied in industry to the simultaneous assessment the level of microbiological contamination and for detection of mites in cereals grains and coffee beans. |
---|---|
AbstractList | A novel approach based on headspace solid-phase microextraction (HS-SPME) combined with comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry (GCGC-ToFMS) was developed for the simultaneous screening of microbial and mite contamination level in cereals and coffee beans. The proposed approach emerges as a powerful tool for the rapid assessment of the microbial contamination level (ca. 70 min versus ca. 72 to 120 h for bacteria and fungi, respectively, using conventional plate counts), and mite contamination (ca. 70 min versus ca. 24 h). A full-factorial design was performed for optimization of the SPME experimental parameters. The methodology was applied to three types of rice (rough, brown, and white rice), oat, wheat, and green and roasted coffee beans. Simultaneously, microbiological analysis of the samples (total aerobic microorganisms, moulds, and yeasts) was performed by conventional plate counts. A set of 54 volatile markers was selected among all the compounds detected by GCGC-ToFMS. Principal Component Analysis (PCA) was applied in order to establish a relationship between potential volatile markers and the level of microbial contamination. Methylbenzene, 3-octanone, 2-nonanone, 2-methyl-3-pentanol, 1-octen-3-ol, and 2-hexanone were associated to samples with higher microbial contamination level, especially in rough rice. Moreover, oat exhibited a high GC peak area of 2-hydroxy-6-methylbenzaldehyde, a sexual and alarm pheromone for adult mites, which in the other matrices appeared as a trace component. The number of mites detected in oat grains was correlated to the GC peak area of the pheromone. The HS-SPME/GCGC-ToFMS methodology can be regarded as the basis for the development of a rapid and versatile method that can be applied in industry to the simultaneous assessment the level of microbiological contamination and for detection of mites in cereals grains and coffee beans. |
Author | Cunha, Angela Rocha, Silvia M Baptista, Ines Barros, Antonio S Gomes, Newton CM Almeida, Adelaide Salvador, Angelo C |
Author_xml | – sequence: 1 givenname: Angelo surname: Salvador middlename: C fullname: Salvador, Angelo C – sequence: 2 givenname: Ines surname: Baptista fullname: Baptista, Ines – sequence: 3 givenname: Antonio surname: Barros middlename: S fullname: Barros, Antonio S – sequence: 4 givenname: Newton surname: Gomes middlename: CM fullname: Gomes, Newton CM – sequence: 5 givenname: Angela surname: Cunha fullname: Cunha, Angela – sequence: 6 givenname: Adelaide surname: Almeida fullname: Almeida, Adelaide – sequence: 7 givenname: Silvia surname: Rocha middlename: M fullname: Rocha, Silvia M |
BookMark | eNqVjstOw0AMRUcIJFrgD1h4yaYlkymhWSGIeCyoWPDYVm7qoKkmdhlPkPgNvphR4QdYWdf3yD5js8_CZMypLabWXdrzjQyRMUy3eT0tiovaufmeGdnalZOqLNyhGatucuHmVTUy3w0yvEnA5APBU3xH9i0sKOFKgk-kcEPwqrSGJPDs-yEkZJJBwxdcq5IqLHwbZeUxAPI6p0TQCCfsPeerwvBInxTAMzQUKWP3ET3rjm6k64jyD2S9AtrZHpuDDoPSyd88Mmd3ty_Nw2Qb5WMgTcvea0sh_Ios7czVpa2Laub-gf4AvRRihA |
ContentType | Journal Article |
DBID | 7T7 8FD C1K FR3 M7N P64 |
DOI | 10.1371/journal.pone.0059338 |
DatabaseName | Industrial and Applied Microbiology Abstracts (Microbiology A) Technology Research Database Environmental Sciences and Pollution Management Engineering Research Database Algology Mycology and Protozoology Abstracts (Microbiology C) Biotechnology and BioEngineering Abstracts |
DatabaseTitle | Technology Research Database Algology Mycology and Protozoology Abstracts (Microbiology C) Engineering Research Database Industrial and Applied Microbiology Abstracts (Microbiology A) Biotechnology and BioEngineering Abstracts Environmental Sciences and Pollution Management |
DatabaseTitleList | Technology Research Database |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Sciences (General) |
EISSN | 1932-6203 |
GroupedDBID | --- 123 29O 2WC 3V. 53G 5VS 7RV 7T7 7X2 7X7 7XC 88E 8AO 8C1 8CJ 8FD 8FE 8FG 8FH 8FI 8FJ A8Z AAFWJ ABDBF ABIVO ABJCF ABUWG ACGFO ACIHN ACIWK ACPRK ADBBV ADRAZ AEAQA AENEX AFKRA AFPKN AFRAH AHMBA ALIPV ALMA_UNASSIGNED_HOLDINGS AOIJS APEBS ARAPS ATCPS BAWUL BBNVY BBORY BCNDV BENPR BGLVJ BHPHI BKEYQ BPHCQ BVXVI BWKFM C1K CCPQU CS3 D1I D1J D1K DIK DU5 E3Z EAP EAS EBD EMOBN ESTFP ESX EX3 F5P FPL FR3 FYUFA GROUPED_DOAJ GX1 HCIFZ HH5 HMCUK HYE IAO IEA IHR IHW INH INR IOV IPNFZ IPY ISE ISR ITC K6- KB. KQ8 L6V LK5 LK8 M0K M1P M48 M7N M7P M7R M7S M~E NAPCQ O5R O5S OK1 P2P P62 P64 PATMY PDBOC PIMPY PQQKQ PROAC PSQYO PTHSS PYCSY RIG RNS RPM SV3 TR2 UKHRP WOQ WOW ~02 ~KM |
ID | FETCH-proquest_miscellaneous_14392190643 |
IEDL.DBID | M48 |
IngestDate | Thu Jul 25 04:48:46 EDT 2024 |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 4 |
Language | English |
LinkModel | DirectLink |
MergedId | FETCHMERGED-proquest_miscellaneous_14392190643 |
Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 content type line 23 ObjectType-Feature-2 |
PQID | 1439219064 |
PQPubID | 23462 |
ParticipantIDs | proquest_miscellaneous_1439219064 |
PublicationCentury | 2000 |
PublicationDate | 20130401 |
PublicationDateYYYYMMDD | 2013-04-01 |
PublicationDate_xml | – month: 04 year: 2013 text: 20130401 day: 01 |
PublicationDecade | 2010 |
PublicationTitle | PloS one |
PublicationYear | 2013 |
SSID | ssj0053866 |
Score | 3.767963 |
Snippet | A novel approach based on headspace solid-phase microextraction (HS-SPME) combined with comprehensive two-dimensional gas chromatography-time-of-flight mass... |
SourceID | proquest |
SourceType | Aggregation Database |
SubjectTerms | Oryza sativa Triticum aestivum |
Title | Can Volatile Organic Metabolites Be Used to Simultaneously Assess Microbial and Mite Contamination Level in Cereal Grains and Coffee Beans? e59338 |
URI | https://search.proquest.com/docview/1439219064 |
Volume | 8 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwjV1LS8NAEB76uHgR6wOfYQQP9dBikm2aHqTY0Adii6iV3mSTbqAQEm0i2Is_wl_szCb1oqKXhcDuDruzk_mGeQGckYp3SFGFDUf4XFTbDRpuwME0F9I2RUv4tq-rfU6c0VRcz1qzEqx7thYXmP5o2nE_qekyar69rLok8Je6a0PbXC9qPiexauoedbZbhqolbMFvfiy-_Aok3Y5TJND9tvLbT1lrmsEWbBYQEa9yntagpOJtqBVCmGK9qBR9vgMfnozxMeFwtkhhnlYZ4FhlxFnOLU6xp3CaqjlmCd4vOHhQxops_WiFubcXxwtdiYnoyXhOX5lCLlglOUKGeYY3HFWEixg9RfgywiH3lEj1bC8JQ6WIBqm7Lip9rl2oD_oP3qixPt0TPST2DuSEyQYgqETwgCDKHlRiupF9QMJXgQjCTmB1JBlypis70gxdy28F0haBfQCnf253-I85R7Bh6T4THBJzDJVs-apOSNtnvgHl9qxNo-uZPA6GBlR7_cntnaHtZ0MzmMf3_icXS7fI |
link.rule.ids | 315,786,790,870,24346,27957,27958,31755,33302,33409,33780 |
linkProvider | Scholars Portal |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Can+Volatile+Organic+Metabolites+Be+Used+to+Simultaneously+Assess+Microbial+and+Mite+Contamination+Level+in+Cereal+Grains+and+Coffee+Beans%3F+e59338&rft.jtitle=PloS+one&rft.au=Salvador%2C+Angelo+C&rft.au=Baptista%2C+Ines&rft.au=Barros%2C+Antonio+S&rft.au=Gomes%2C+Newton+CM&rft.date=2013-04-01&rft.eissn=1932-6203&rft.volume=8&rft.issue=4&rft_id=info:doi/10.1371%2Fjournal.pone.0059338&rft.externalDBID=NO_FULL_TEXT |