In Vitro Differentiation Bone Marrow Stem Cell Derived Neural Stem Cell into Oligodendrocyte-Like Cell

• Published in: Cell journal (Yakhteh) Vol. 14; p. 25
• Main Authors: Abbaszade, H, Tiraihi, T, Delshad, A R, Kazemi, H
• Format: Journal Article
• Language: English
• Published: 01.01.2012
• Subjects: Bone marrow; CD45 antigen; CD90 antigen; Differentiation; Epidermal growth factor; Fibroblast growth factor 2; Fibronectin; Glial fibrillary acidic protein; heregulin; mRNA; Neural stem cells; Neurofilaments; Neuronal-glial interactions; neurospheres; Oct-4 protein; Oligodendrocyte-myelin glycoprotein; Oligodendrocytes; Platelet-derived growth factor; Polymerase chain reaction
• ISSN: 2228-5806

Objective: The induction of Bone marrow stem cells in to neural and glial phenotypes was ducomented, however, the yield was low. in this investigation we tried to increase the yield of oligodendrocyte -like cells, by production of neurospheres derived from Bone marrow stem cell then neural stem cell was isolated the from neurospheres and induced in to oligodendrocyte -like cells. Materials and Methods: In this study BMSCs of adult female rats were expanded and then was induced in to form neurospheres in the presence of B27, epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF), and subsequent isolation of neural stem cells. This was followed by induction into oligodendrocyte like cells with heregulin, PDGF-AA, BFGF and T3. Which are used for transdifferentiation of neuroepithelial cells into oligodendrocyte like cells. After induction the expression profile showed O1, O4, S100 and GFAP neurofilaments. Also the expression of OCT4, B2M, MOG and PDGFR-alpha mRNA was noticed using RT-PCR technique. Results: The Immunohistochemical and RT-PCR studies showed expression the using fibronectin, CD44, CD90, CD45 and Oct-4 and B2M which indicated that following 4 super(th) passage, a 94% percentage of cultured cells were BMSCs. The yield of oligodendrocyte like cells was about 82% using O1, O4 to characterized the oligodendrocyte -like cell. The expression of MOG and PDGFR-ALPHA in oligodendrocyte like cells, 6 day after treatment. Conclusion: We demonstrated that neurospheres derived BMSCs could be differentiated into neural stem cells then oligodendrocyte like cells in the presence of heregulin, bFGF, PDGF and T3.