The cytostatic potential confirmation of some fungal autochthonous biopreparations upon HeLa neoplastic cells cultures

• Published in: Analele științifice ale Universității "Al. I. Cuza" din Iași. Serie nouă. Genetică și biologie moleculară Vol. 11; no. 1
• Main Authors: Rotinberg, Pincu, Mihai, Cosmin Teodor, Gherghel, Daniela, Capraru, Gabriela, Iurea, Dorina, Rosu, Craita Maria, Stefania, Surdu
• Format: Journal Article
• Language: English
• Published: 01.01.2010
• Subjects: Age; Ale; Cell culture; Claviceps purpurea; Developmental stages; Media (culture); Ontogeny; Protein biosynthesis
• ISSN: 1582-3571

Some biopreparations of alkaloid-ergolinic nature have been extracted from hyphal and supernatant components, which were centrifugally separated from the submerged culture media of three strains of Claviceps purpurea (T1-3, T2-1 and T13-1), these being in different ontogenetic development stages (4, 6, 8, 10, and 12 days, respectively). In vitro testing of their interaction with cellular protein synthesis process of HeLa neoplastic cells cultures, has highlighted the cellular protein biosynthesis alteration, modifications of the protein dynamics sense and amplitude, as well as the cell cultures development inhibition. The protein synthesis inhibitory impact has confirmed the cytostatic action of these natural bioproducts, their cytostatic effectiveness being dependent of the Claviceps purpurea (T1-3, T2-1and T13-1) strains specificity, the strain ontogenetic age, the biochemical nature of the intracellularly synthesized, stocked and extracellularly discharged substratum, as well as of their obtaining sources.