Long-term n-3 FA deficiency modifies peroxisome proliferator-activated receptor [beta] mRNA abundance in rat ocular tissues
Peroxisomal proliferator-activated receptors (PPAR) are a FA-response system involved in diverse cellular responses. FA regulate PPAR activity and modulate PPAR mRNA abundance. Increasing evidence indicates that PUFA are required for optimal neuronal development and function. To gain insight into th...
Saved in:
Published in | Lipids Vol. 37; no. 4; p. 367 |
---|---|
Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Heidelberg
Springer Nature B.V
01.04.2002
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | Peroxisomal proliferator-activated receptors (PPAR) are a FA-response system involved in diverse cellular responses. FA regulate PPAR activity and modulate PPAR mRNA abundance. Increasing evidence indicates that PUFA are required for optimal neuronal development and function. To gain insight into the mechanism for nutrition-induced impairment of neuronal development and function we investigated the effect of chronic n-3 FA deficiency on PPAR mRNA levels in rat brain and ocular tissues. Rats were fed for three generations a diet designed to reduce DHA levels in tissues, and the abundance of PPARα and PPARβ transcripts was measured by hybridization with specific probes. Chronic consumption of the α-linolenic acid (LNA)-insufficient diet caused a remarkable modification in DHA content in membrane phospholipids. The results reported here indicate that PPARα mRNA levels did not exhibit significant variation in ocular, hepatic, or nervous tissues from rats fed the experimental diet. In contrast, PPARβ mRNA normalized to β-actin mRNA was 21% higher in ocular tissue from F3 generation rats consuming the LNA-deficient diet but was independent of diet in hepatic and nervous tissues. The absolute abundance of PPARβ transcripts showed a 17% increase in ocular tissue from rats consuming the LNA-deficient diet (F3 generation). The biological significance of the reported changes in PPARβ mRNA in ocular tissue remains to be determined.[PUBLICATION ABSTRACT] |
---|---|
AbstractList | Peroxisomal proliferator-activated receptors (PPAR) are a FA-response system involved in diverse cellular responses. FA regulate PPAR activity and modulate PPAR mRNA abundance. Increasing evidence indicates that PUFA are required for optimal neuronal development and function. To gain insight into the mechanism for nutrition-induced impairment of neuronal development and function we investigated the effect of chronic n-3 FA deficiency on PPAR mRNA levels in rat brain and ocular tissues. Rats were fed for three generations a diet designed to reduce DHA levels in tissues, and the abundance of PPARα and PPARβ transcripts was measured by hybridization with specific probes. Chronic consumption of the α-linolenic acid (LNA)-insufficient diet caused a remarkable modification in DHA content in membrane phospholipids. The results reported here indicate that PPARα mRNA levels did not exhibit significant variation in ocular, hepatic, or nervous tissues from rats fed the experimental diet. In contrast, PPARβ mRNA normalized to β-actin mRNA was 21% higher in ocular tissue from F3 generation rats consuming the LNA-deficient diet but was independent of diet in hepatic and nervous tissues. The absolute abundance of PPARβ transcripts showed a 17% increase in ocular tissue from rats consuming the LNA-deficient diet (F3 generation). The biological significance of the reported changes in PPARβ mRNA in ocular tissue remains to be determined.[PUBLICATION ABSTRACT] |
Author | Greiner, Rebecca S Martínez, Jessica I Rojas, Cecilia V Uauy, Ricardo Fuenzalida, Lidia C Salem, Norman |
Author_xml | – sequence: 1 givenname: Cecilia surname: Rojas middlename: V fullname: Rojas, Cecilia V – sequence: 2 givenname: Rebecca surname: Greiner middlename: S fullname: Greiner, Rebecca S – sequence: 3 givenname: Lidia surname: Fuenzalida middlename: C fullname: Fuenzalida, Lidia C – sequence: 4 givenname: Jessica surname: Martínez middlename: I fullname: Martínez, Jessica I – sequence: 5 givenname: Norman surname: Salem fullname: Salem, Norman – sequence: 6 givenname: Ricardo surname: Uauy fullname: Uauy, Ricardo |
BookMark | eNqNjUFLxDAUhB-ygl317vHhPZq0KZseF3HxIB7Em8iSTV_lLW1Sk1QU_7w5-AM8DTPfDLOGlQ-eAK6UvFFSbm6TUroVUtZCdlILfQKValsjukZuVlAVUMJaqjNYp3QsVumureDnMfh3kSlO6EWDuy32NLBj8u4bp9DzwJRwphi-OIWJcI5h5IGizSEK6zJ_2kw9RnI0lwhfD5TtG07PT1u0h8X31jtC9lgWGNwy2oiZU1ooXcDpYMdEl396Dte7-5e7B1E-PgrP-2NYoi9ob4xRja470_yr9Av7llZ- |
ContentType | Journal Article |
Copyright | AOCS Press 2002 |
Copyright_xml | – notice: AOCS Press 2002 |
DBID | 3V. 7QL 7QP 7QR 7T7 7TK 7U9 7X7 7XB 88A 88E 8AO 8C1 8FD 8FE 8FH 8FI 8FJ 8FK ABUWG AFKRA AZQEC BBNVY BENPR BHPHI BKSAR C1K CCPQU DWQXO FR3 FYUFA GHDGH GNUQQ H94 HCIFZ K9. LK8 M0S M1P M7N M7P P64 PCBAR PQEST PQQKQ PQUKI PRINS |
DOI | 10.1007/s1145-002-0904-4 |
DatabaseName | ProQuest Central (Corporate) Bacteriology Abstracts (Microbiology B) Calcium & Calcified Tissue Abstracts Chemoreception Abstracts Industrial and Applied Microbiology Abstracts (Microbiology A) Neurosciences Abstracts Virology and AIDS Abstracts Health & Medical Collection ProQuest Central (purchase pre-March 2016) Biology Database (Alumni Edition) Medical Database (Alumni Edition) ProQuest Pharma Collection Public Health Database Technology Research Database ProQuest SciTech Collection ProQuest Natural Science Collection Hospital Premium Collection Hospital Premium Collection (Alumni Edition) ProQuest Central (Alumni) (purchase pre-March 2016) ProQuest Central (Alumni) ProQuest Central ProQuest Central Essentials Biological Science Collection ProQuest Central Natural Science Collection Earth, Atmospheric & Aquatic Science Collection Environmental Sciences and Pollution Management ProQuest One Community College ProQuest Central Korea Engineering Research Database Health Research Premium Collection Health Research Premium Collection (Alumni) ProQuest Central Student AIDS and Cancer Research Abstracts SciTech Premium Collection ProQuest Health & Medical Complete (Alumni) Biological Sciences Health & Medical Collection (Alumni Edition) PML(ProQuest Medical Library) Algology Mycology and Protozoology Abstracts (Microbiology C) Biological Science Database Biotechnology and BioEngineering Abstracts Earth, Atmospheric & Aquatic Science Database ProQuest One Academic Eastern Edition (DO NOT USE) ProQuest One Academic ProQuest One Academic UKI Edition ProQuest Central China |
DatabaseTitle | ProQuest Central Student Technology Research Database ProQuest Central Essentials ProQuest Health & Medical Complete (Alumni) ProQuest Central (Alumni Edition) SciTech Premium Collection ProQuest One Community College ProQuest Natural Science Collection ProQuest Pharma Collection ProQuest Central China Environmental Sciences and Pollution Management ProQuest Biology Journals (Alumni Edition) ProQuest Central Earth, Atmospheric & Aquatic Science Collection Health Research Premium Collection Health and Medicine Complete (Alumni Edition) Natural Science Collection ProQuest Central Korea Bacteriology Abstracts (Microbiology B) Algology Mycology and Protozoology Abstracts (Microbiology C) Biological Science Collection AIDS and Cancer Research Abstracts Chemoreception Abstracts Industrial and Applied Microbiology Abstracts (Microbiology A) ProQuest Medical Library (Alumni) ProQuest Public Health Virology and AIDS Abstracts ProQuest Biological Science Collection ProQuest One Academic Eastern Edition Earth, Atmospheric & Aquatic Science Database ProQuest Hospital Collection Health Research Premium Collection (Alumni) Biological Science Database ProQuest SciTech Collection Neurosciences Abstracts ProQuest Hospital Collection (Alumni) Biotechnology and BioEngineering Abstracts ProQuest Health & Medical Complete ProQuest Medical Library ProQuest One Academic UKI Edition Engineering Research Database ProQuest One Academic Calcium & Calcified Tissue Abstracts ProQuest Central (Alumni) |
DatabaseTitleList | ProQuest Central Student |
Database_xml | – sequence: 1 dbid: BENPR name: ProQuest Central url: https://www.proquest.com/central sourceTypes: Aggregation Database |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Anatomy & Physiology |
EISSN | 1558-9307 |
ExternalDocumentID | 2446678121 |
GroupedDBID | --- -4W -58 -5G -BR -Y2 -~C -~X .55 .86 .GJ .VR 06C 06D 0R~ 0VY 199 1N0 1OC 1SB 203 29L 29~ 2J2 2JY 2KG 2KM 2LR 2VQ 2~H 30V 33P 3V. 4.4 408 40D 40E 53G 5GY 5RE 5VS 67Z 6NX 78A 7QL 7QP 7QR 7T7 7TK 7U9 7X7 7XB 875 88A 88E 8AO 8C1 8CJ 8FD 8FE 8FH 8FI 8FJ 8FK 8TC 8UJ 95- 95. 95~ 96X AAAVM AABHQ AAHBH AAHHS AAIAL AAJKR AANLZ AANXM AARHV AARTL AASGY AAWCG AAXRX AAYIU AAYQN AAYTO AAZKR ABCUV ABHLI ABJNI ABJOX ABMNI ABNWP ABPLI ABTEG ABTHY ABTMW ABUWG ACAHQ ACBXY ACCFJ ACCZN ACGFS ACHXU ACIWK ACKNC ACOMO ACPOU ACPRK ACXBN ACXQS ADBBV ADHIR ADINQ ADKPE ADKYN ADOZA ADRFC ADXAS ADZMN AEBTG AEEZP AEGAL AEGNC AEIGN AEJHL AEKMD AENEX AEOHA AEPYU AEQDE AETLH AEUYR AFBBN AFFPM AFGCZ AFKRA AFLOW AFRAH AFWTZ AFZKB AGAYW AGJBK AGQMX AGWIL AGWZB AGYKE AHBTC AHBYD AHKAY AHMBA AHSBF AHYZX AITYG AIURR AIWBW AJBDE AJBLW AJRNO AKMHD ALIPV ALMA_UNASSIGNED_HOLDINGS ALUQN ALWAN AMKLP AMYDB ARMRJ ASPBG AVWKF AZFZN AZQEC B-. BA0 BBNVY BENPR BFHJK BGNMA BHPHI BKSAR BPHCQ BVXVI C1K CAG CCPQU COF CS3 CSCUP D1J DCZOG DRFUL DRSTM DU5 DWQXO EBS EJD EMOBN ESBYG F5P FEDTE FIGPU FNLPD FR3 FRRFC FWDCC FYUFA G-Y G-Z GGCAI GGRSB GNUQQ GQ6 GQ7 H13 H94 HCIFZ HF~ HG5 HG6 HGLYW HMCUK HMJXF HRMNR HVGLF HZ~ IJ- ITM IXC IXE IZQ I~X I~Z J-C J0Z JBSCW K9. KDC KOV LATKE LEEKS LK8 LUTES LYRES M0L M1P M4Y M7N M7P MA- MEWTI N2Q N9A NB0 NF0 NU0 O9- O93 O9G O9I O9J OAM P19 P2P P2W P64 P9N PCBAR PF0 PQEST PQQKQ PQUKI PRINS PROAC PSQYO Q2X QOR QOS R89 R9I RGG ROL RPX RSV S16 S27 S3B SAP SCM SDH SDM SHX SJN SNE SNX SOJ SUPJJ SZN T13 TSG TSK TSV TUC U2A U9L UG4 UKHRP VC2 W48 WH7 WK8 WOHZO WXSBR WYJ X7M Y6R Z45 Z7U Z7V Z7W Z7Y Z87 Z8O Z8P Z8Q Z8S Z91 ZCG ZGI ZOVNA ZZTAW ~02 ~EX ~KM |
ID | FETCH-proquest_journals_8881342983 |
IEDL.DBID | 8C1 |
ISSN | 0024-4201 |
IngestDate | Fri Sep 13 06:26:31 EDT 2024 |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 4 |
Language | English |
LinkModel | DirectLink |
MergedId | FETCHMERGED-proquest_journals_8881342983 |
PQID | 888134298 |
PQPubID | 35263 |
ParticipantIDs | proquest_journals_888134298 |
PublicationCentury | 2000 |
PublicationDate | 20020401 |
PublicationDateYYYYMMDD | 2002-04-01 |
PublicationDate_xml | – month: 04 year: 2002 text: 20020401 day: 01 |
PublicationDecade | 2000 |
PublicationPlace | Heidelberg |
PublicationPlace_xml | – name: Heidelberg |
PublicationTitle | Lipids |
PublicationYear | 2002 |
Publisher | Springer Nature B.V |
Publisher_xml | – name: Springer Nature B.V |
SSID | ssj0021495 |
Score | 3.3378198 |
Snippet | Peroxisomal proliferator-activated receptors (PPAR) are a FA-response system involved in diverse cellular responses. FA regulate PPAR activity and modulate... |
SourceID | proquest |
SourceType | Aggregation Database |
StartPage | 367 |
SubjectTerms | Proteins Rodents |
Title | Long-term n-3 FA deficiency modifies peroxisome proliferator-activated receptor [beta] mRNA abundance in rat ocular tissues |
URI | https://www.proquest.com/docview/888134298/abstract/ |
Volume | 37 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV3BSsNAEB1qe_EiahW1WgYRb0sTu2mak8TSWkSLFIWCSNlNNtJDkmqjKP68M9u0F6HXLFlIZnizM_PeDsBF1NEycQNfuEHkC6lNIBRlAUIHnqeUohBsG-0Po87wWd5NvEkFbldaGKZVrjDRAnWcR1wjb1Gm5rYJPLstpbkIEBWt6_m74PFR3GYtZ2lsQc0lD-UZDt3emutxxWnAkushhaSQV_Yrlxo6V3qCccEJHFr9h8k20Ax2Yac8IWK4NOkeVEy2D_Uwo-w4_cFLtJxNWwyvw-99nr0JRlfMRBsHIcaGr4RgPSWmeTxLKBFGvgv8e7bIU4NzHtKTGNtbF6xp-KKzZowEe2ZOj_BFm0K9Yjoehag0q0TIKXCWIb2BueWsYmFttTiA80H_qTcUqw-Zln65mK7_YvsQqlmemSPAKHJ0opTRKtCsMw1M4sikTegjE0d34mNobNjoZONqA7bt-BRLdTmFavHxac4oihe6CVv-xG9aOzWhdtMfPY7_APEmpco |
link.rule.ids | 315,786,790,12083,12250,21416,27957,27958,31754,33301,33779,43345,43614,43840,74102,74371,74659 |
linkProvider | ProQuest |
linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV3fS8MwEA46H_RF1Cnq_HGI-BZsabqZJynimLrtQSYMREbSJrKHptNWUfznvcs6fRB8bWggufDdfbnvcoydpm0tbCg7PJRphwttJFfIAriWcayUQhfsE-2DYbv3IG7H8bjW5pS1rHKBiR6osyKlO_JzZGphhOB5cTl74dQ0ipKrdQeNZbYiokiQoq8z_uVbFPzPFR6CC3R0dZZyXjkXipgTGgQywNE_SOzdS3eDrddxISRzQ26yJeO2WDNxyInzTzgDr9T0V-BN9tUv3DMnTAXHI-gmkBl6CIKqKCEvsqlF-gv0AvjHtCxyAzNqzWONz6hzqmR4xwgzAwQ7M8NP8KhNpZ4gvx8moDTVhuBRgKkD_AMKr1SFyluo3GYn3evRVY8vFjKpT2M5-dm7aIc1XOHMLoM0DbRVymglNVWXSmMDYSPEHGED3c72WOufifb_HT1mq73RoD_p3wzvWmzNN1DxYpcD1qhe38wh-vFKH3lrfQMThaK0 |
linkToPdf | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV3fS8MwEA46QXwRdYo6fxwivoW1NN3MkxS1TJ1DRGEgMpI2kT00nbaK4j_vJcv0QdhrQwPJhe_y5b67I-Q460imQ96lIc-6lEnFqUAWQCWPYyEEumAXaL8ddHqP7HoYD31JocrLKmeY6IA6LzP7Rt5GphZGCJ6nbe1VEXcX6dnkldoGUjbQ6rtpLJIldJKBbWbQHf5xL0sEpmoPRhk6PR-xnGbRhSymFhkCHuDoP1R2riZdI6v-jgjJ1KjrZEGZDdJMDPLj4gtOwKk23XN4k3z3S_NCLb6CoRGkCeTKFoWwGZVQlPlYIxUGWw38c1yVhYKJbdOjlYuuU5vV8IG3zRwQ-NQEP8GTVLV4huJ-kICQNk8EjwWMDeAfUDrVKtTOWtUmOUovH857dLaQkT-Z1eh3H6Mt0jClUdsEsiyQWgglBZc205QrHTAdIf4wHchOvkNacybanTt6SJbRUKP-1eCmRVZcLxWne9kjjfrtXe2jS6_lgTPWD_7ypuA |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Long-term+n-3+FA+deficiency+modifies+peroxisome+proliferator-activated+receptor+%5Bbeta%5D+mRNA+abundance+in+rat+ocular+tissues&rft.jtitle=Lipids&rft.au=Rojas%2C+Cecilia+V&rft.au=Greiner%2C+Rebecca+S&rft.au=Fuenzalida%2C+Lidia+C&rft.au=Mart%C3%ADnez%2C+Jessica+I&rft.date=2002-04-01&rft.pub=Springer+Nature+B.V&rft.issn=0024-4201&rft.eissn=1558-9307&rft.volume=37&rft.issue=4&rft.spage=367&rft_id=info:doi/10.1007%2Fs1145-002-0904-4&rft.externalDBID=HAS_PDF_LINK&rft.externalDocID=2446678121 |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0024-4201&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0024-4201&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0024-4201&client=summon |