Enhanced biodesulfurization by magnetic immobilized Rhodococcus erythropolis LSSE8-1-vgb assembled with nano-[gamma]-Al2O3

• Published in: World journal of microbiology & biotechnology Vol. 27; no. 2; p. 299
• Main Authors: Zhang, Ting, Li, Wang-liang, Chen, Xiao-xin, Tang, Huang, Li, Qiang, Xing, Jian-min, Liu, Hui-zhou
• Format: Journal Article
• Language: English
• Published: Oxford Springer Nature B.V 01.02.2011
• Subjects: Adsorbents; Adsorption; Biotechnology; Chromatography; Desulfurization; Diesel fuels; Laboratories; Magnetic fields; Nanoparticles; Studies; Sulfur content; China
• DOI: 10.1007/s11274-010-0459-7
• ISSN: 0959-3993

Biodesulfurization activity can be enhanced by assembling nano-γ-Al^sub 2^O3 particles on the magnetic immobilized Rhodococcus erythropolis LSSE8-1-vgb. The cells can be collected and reused conveniently by an external magnetic field. Firstly, cells were magnetic immobilized by coating with Fe^sub 3^O^sub 4^ nano-particles. The optimal ratio of cells to magnetic Fe^sub 3^O^sub 4^ nano-particles was determined to be 50:1 (g/g). Then nano-γ-Al^sub 2^O3 adsorbents were assembled onto the cells to enhance the desulfurization activity. The nano-γ-Al^sub 2^O3 adsorbent had the largest pore volume as well as specific surface area, and the strongest electrostatics interaction with microbial cell, and cells assembled with this nano-adsorbent performed the highest desulfurization activity. The activity of magnetic immobilized cells assembled with adsorbents was tested in desulfurization of model oil. The desulfurization rate was raised by nearly 20% when the amount ratio of magnetic particles to adsorbents was 1:5 (g/g). These cells can be reused. The activity decreased less than 10% through out three desulfurization-activation-reuse recycles. [PUBLICATION ABSTRACT]