Production of l-Lysine from starch by Corynebacterium glutamicum displaying [alpha]-amylase on its cell surface

• Published in: Applied microbiology and biotechnology Vol. 74; no. 6; p. 1213
• Main Authors: Tateno, Toshihiro, Fukuda, Hideki, Kondo, Akihiko
• Format: Journal Article
• Language: English
• Published: Heidelberg Springer Nature B.V 01.04.2007
• Subjects: Amino acids; Bacteria; Carbon; Carbon sources; Enzymes; Fermentation; Microbiology; Starch

We engineered a Corynebacterium glutamicum strain displaying alpha-amylase from Streptococcus bovis 148 (AmyA) on its cell surface to produce amino acids directly from starch. We used PgsA from Bacillus subtilis as an anchor protein, and the N-terminus of alpha-amylase was fused to the PgsA. The gen...