The complexity and dynamics of Plasmodium vivax infections in Papua New Guinean children
Plasmodium vivax is the most geographically widespread of the human malaria species and presents a serious public health challenge in South America, Central America, Asia and the southwest Pacific. In Papua New Guinea (PNG), malaria is the second leading cause of death. In this study, we have explor...
Saved in:
Main Author | |
---|---|
Format | Dissertation |
Language | English |
Published |
ProQuest Dissertations & Theses
01.01.2007
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | Plasmodium vivax is the most geographically widespread of the human malaria species and presents a serious public health challenge in South America, Central America, Asia and the southwest Pacific. In Papua New Guinea (PNG), malaria is the second leading cause of death. In this study, we have explored the complexity and dynamics of P. vivax infections. We have shown that P. vivax infections are extremely complex consisting of up to 23 unique parasites in Papua New Guinean children---2- to 4-fold higher than previously reported. We have developed a technique to identify the most abundant vivax clone in the infection based on the highly polymorphic P. vivax Duffy binding protein region II ( PvdbpII). Using this technique, we have genotyped the PvdbpII in P. vivax positive samples collected biweekly from 206 Papua New Guinean children for 6 months after treatment to clear blood stage infections. Since the PvDBPII is the only known ligand for the Duffy antigen (DARC) which is essential for merozoite invasion into erythrocytes, we hypothesized that children with strong antibody responses to a specific PvDBPII allelic variant would have longer times to re-infection with that same allele. We found no evidence to support strain-specific immunity to PvDBPII, though the functional significance of the antibodies measured is unclear. We further hypothesized that certain PvDBPII alleles could influence P. vivax parasitemia---some alleles might bind more strongly to the DARC on the surface of the erythrocyte and influence invasion to occur more quickly and/or other alleles might be less immunogenic and be able to avoid the host immune response. We found no evidence that the PvDBPII allele had any significant affect on P. vivax parasitemia. Therefore, P. vivax infections are complex and re-infections occur quickly after treatment in holoendemic areas such as PNG; a better understanding, though, of the dynamics of mixed Plasmodium infections is needed before embarking on a vaccine for P. vivax. |
---|---|
AbstractList | Plasmodium vivax is the most geographically widespread of the human malaria species and presents a serious public health challenge in South America, Central America, Asia and the southwest Pacific. In Papua New Guinea (PNG), malaria is the second leading cause of death. In this study, we have explored the complexity and dynamics of P. vivax infections. We have shown that P. vivax infections are extremely complex consisting of up to 23 unique parasites in Papua New Guinean children---2- to 4-fold higher than previously reported. We have developed a technique to identify the most abundant vivax clone in the infection based on the highly polymorphic P. vivax Duffy binding protein region II ( PvdbpII). Using this technique, we have genotyped the PvdbpII in P. vivax positive samples collected biweekly from 206 Papua New Guinean children for 6 months after treatment to clear blood stage infections. Since the PvDBPII is the only known ligand for the Duffy antigen (DARC) which is essential for merozoite invasion into erythrocytes, we hypothesized that children with strong antibody responses to a specific PvDBPII allelic variant would have longer times to re-infection with that same allele. We found no evidence to support strain-specific immunity to PvDBPII, though the functional significance of the antibodies measured is unclear. We further hypothesized that certain PvDBPII alleles could influence P. vivax parasitemia---some alleles might bind more strongly to the DARC on the surface of the erythrocyte and influence invasion to occur more quickly and/or other alleles might be less immunogenic and be able to avoid the host immune response. We found no evidence that the PvDBPII allele had any significant affect on P. vivax parasitemia. Therefore, P. vivax infections are complex and re-infections occur quickly after treatment in holoendemic areas such as PNG; a better understanding, though, of the dynamics of mixed Plasmodium infections is needed before embarking on a vaccine for P. vivax. |
Author | Cole-Tobian, Jennifer L |
Author_xml | – sequence: 1 givenname: Jennifer surname: Cole-Tobian middlename: L fullname: Cole-Tobian, Jennifer L |
BookMark | eNqNjrkOwjAQRC0BElf-YUWP5GCU4BpxVIiCgg6ZZCMW2esQxxx_Two-gGI0T5pXzFj02TP2RKLzVZpKrVWWLpZDkYRAVymlVkouFyNxPt0QCu9qi29qP2C4hPLDxlERwFdwtCY4X1J08KSneQNxhUVLnkOHcDR1NHDAF-wiMRqG4ka2bJCnYlAZGzD59UTMtpvTej-vG_-IGNrL3ceGu-nSPcmzLkr9JX0Bgd9EYg |
ContentType | Dissertation |
Copyright | Database copyright ProQuest LLC; ProQuest does not claim copyright in the individual underlying works. |
Copyright_xml | – notice: Database copyright ProQuest LLC; ProQuest does not claim copyright in the individual underlying works. |
DBID | 053 0BH 0FD 3V. 7X7 7XB 8FI 8FJ 8FK AAENX ABUWG ADAJB ADZZV AFCXM AFKRA AGBVP AQTIP BENPR CBPLH CCPQU EU9 FYUFA G20 GHDGH K9. M0S M8- PQCXX PQEST PQQKQ PQUKI PRINS |
DatabaseName | Dissertations & Theses Europe Full Text: Science & Technology ProQuest Dissertations and Theses Professional Dissertations & Theses @ Case Western Reserve University ProQuest Central (Corporate) Health & Medical Collection ProQuest Central (purchase pre-March 2016) Hospital Premium Collection Hospital Premium Collection (Alumni Edition) ProQuest Central (Alumni) (purchase pre-March 2016) Health & Medical Collection - hybrid linking ProQuest Central (Alumni) Health Research Premium Collection (Alumni) - hybrid linking ProQuest Central (Alumni) - hybrid linking Health Research Premium Collection - hybrid linking ProQuest Central UK/Ireland Health & Medical Collection (Alumni) - hybrid linking ProQuest Women's & Gender Studies - hybrid linking ProQuest Central ProQuest Dissertations & Theses Global: The Sciences and Engineering Collection ProQuest One Community College ProQuest Dissertations & Theses A&I Health Research Premium Collection ProQuest Dissertations & Theses Global Health Research Premium Collection (Alumni) ProQuest Health & Medical Complete (Alumni) Health & Medical Collection (Alumni Edition) ProQuest Dissertations and Theses A&I: The Sciences and Engineering Collection ProQuest Central - hybrid linking ProQuest One Academic Eastern Edition (DO NOT USE) ProQuest One Academic ProQuest One Academic UKI Edition ProQuest Central China |
DatabaseTitle | ProQuest One Academic Eastern Edition ProQuest Health & Medical Complete (Alumni) ProQuest Central (Alumni Edition) ProQuest One Community College ProQuest Hospital Collection Health Research Premium Collection (Alumni) ProQuest Dissertations & Theses Global: The Sciences and Engineering Collection ProQuest Dissertations and Theses Professional ProQuest Central China ProQuest Hospital Collection (Alumni) ProQuest Dissertations and Theses A&I: The Sciences and Engineering Collection ProQuest Dissertations & Theses Global Dissertations & Theses Europe Full Text: Science & Technology ProQuest Central ProQuest Health & Medical Complete Health Research Premium Collection ProQuest One Academic UKI Edition Health and Medicine Complete (Alumni Edition) ProQuest One Academic ProQuest Dissertations & Theses A&I ProQuest Central (Alumni) Dissertations & Theses @ Case Western Reserve University |
DatabaseTitleList | ProQuest One Academic Eastern Edition |
Database_xml | – sequence: 1 dbid: BENPR name: ProQuest Central url: https://www.proquest.com/central sourceTypes: Aggregation Database |
DeliveryMethod | fulltext_linktorsrc |
ExternalDocumentID | 1310423561 |
Genre | Dissertation/Thesis |
GroupedDBID | 053 0BH 0FD 123 3V. 7X7 7XB 8FI 8FJ 8FK 8R4 8R5 ABUWG AFKRA BENPR CBPLH CCPQU EU9 FYUFA G20 K9. M8- PQEST PQQKQ PQUKI PRINS Q2X |
ID | FETCH-proquest_journals_3047604733 |
IEDL.DBID | BENPR |
ISBN | 9781109936124 1109936125 |
IngestDate | Fri Sep 13 07:29:19 EDT 2024 |
IsPeerReviewed | false |
IsScholarly | false |
Language | English |
LinkModel | DirectLink |
MergedId | FETCHMERGED-proquest_journals_3047604733 |
PQID | 304760473 |
PQPubID | 18750 |
ParticipantIDs | proquest_journals_304760473 |
PublicationCentury | 2000 |
PublicationDate | 20070101 |
PublicationDateYYYYMMDD | 2007-01-01 |
PublicationDate_xml | – month: 01 year: 2007 text: 20070101 day: 01 |
PublicationDecade | 2000 |
PublicationYear | 2007 |
Publisher | ProQuest Dissertations & Theses |
Publisher_xml | – name: ProQuest Dissertations & Theses |
SSID | ssib000933042 |
Score | 2.96705 |
Snippet | Plasmodium vivax is the most geographically widespread of the human malaria species and presents a serious public health challenge in South America, Central... |
SourceID | proquest |
SourceType | Aggregation Database |
SubjectTerms | Epidemiology Parasitology Pathology |
Title | The complexity and dynamics of Plasmodium vivax infections in Papua New Guinean children |
URI | https://www.proquest.com/docview/304760473/abstract/ |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfR1NS8Mw9LGPi3hRVNTpeIjXUtb0K6eBug88jCIKvY20SWCHtdO1Yz_fl64RQdkhkBAI4SXv-wvg0Qu1p5XUjpCMFBSPhU4Wc0V4FSiWjSJFKGWiLRbh_MN_TYO0AzObC2PCKi1NbAi1LHNjI3eNeyikwVyRGSNAXrnjzadj2kcZN2vbS6MLfW_kG39t_2mySN7-UdxNiU3ODGNvKz7Ztf-HHDc8ZnoGpy-_fOPn0FHFBaT0jNiEfas9yctIej_KQxP5LZYaExJ-16Vc1WvcrXZijza4qtjSFBOxqQUSIcNZTeKkKNAmb1_Cw3Ty_jx37F2W7a_aLn9gwK6gV5SFugbMY0UiFReaOK0fac2FyJgnecwDHWsd38DgyEG3R3cHcHIwZRqLwx30qq9a3RMPrrIhdKM0GrZQ_ga8Y49p |
link.rule.ids | 312,786,790,791,12083,21416,31754,33779,43345,43840,74102,74659 |
linkProvider | ProQuest |
linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV3NS8MwFH_oPCheFBV1fjzEaxGb9CMnD-qsOscOE3or6ZLADms3u479-b50jQyEHQIJgZDP9_nLewD3fmh8o5XxpGKkoPgs9PJYaHpXgWb5Y6TpSVm0xSBMvvlHGqQtNqdqYZWOJjaEWpVjayN_sO6hkAp7ms09mzTKOlfbDBq7sMcZ4xbRF6Wb0dScsm7DagpmmXkb5cm1-T8S3PCV3hEcvmz4w49hRxcnkNLRYQP11iuSkZF0fVTrxPEVlgaHJPBOSzWpp7icLOUKHaCqqKiKQzmrJRLxwreaREhZoPuwfQp3vdfRc-K5uWTtTaqyv3WzM-gUZaHPAcexJjFKSEPclUfGCClz5isRi8DExsQX0N0y0OXW3lvYT0Zf_az_PvjswsHalGktDlfQWfzU-pp48CK_aXb6F9i7jE8 |
linkToPdf | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV3dS8MwED90AxFfFBV1fhziaxk2_cqToNucH5QiCn0r6ZLAHtZOu479-V66VARlD4GEQAiX5D5_uQO4cQPtaiW1IyQjA8VlgZNHXNG78hXLb0NFT8qgLeJg_OE9p35qUwpVFlbZ8sSGUctyYnzkfRMeCqixvraoiGQwupt_OqaAlAm02moa29ANvcCnC969H8bJ2z-mu0myyZkR7TbnUzv2_jDkRsqM9mFv8Cs6fgBbqjiElA4SG-C3WpHGjGT5o1yXka-w1JiQ-jsr5bSe4XK6FCts4VVFRV1MxLwWSKwMH2tSKEWB7fftI7geDd8fxk67l8zeqyr7oQI7hk5RFuoEcBIpUqq40CRrvVBrLkTOXMkj7utI6-gUehsWOts4ewU7RObs9Sl-6cHu2q9p3A_n0Fl81eqCBPIiv7Sk_gY7qpHy |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adissertation&rft.genre=dissertation&rft.title=The+complexity+and+dynamics+of+Plasmodium+vivax+infections+in+Papua+New+Guinean+children&rft.DBID=053%3B0BH%3B0FD%3B3V.%3B7X7%3B7XB%3B8FI%3B8FJ%3B8FK%3BAAENX%3BABUWG%3BADAJB%3BADZZV%3BAFCXM%3BAFKRA%3BAGBVP%3BAQTIP%3BBENPR%3BCBPLH%3BCCPQU%3BEU9%3BFYUFA%3BG20%3BGHDGH%3BK9.%3BM0S%3BM8-%3BPQCXX%3BPQEST%3BPQQKQ%3BPQUKI%3BPRINS&rft.PQPubID=18750&rft.au=Cole-Tobian%2C+Jennifer+L&rft.date=2007-01-01&rft.pub=ProQuest+Dissertations+%26+Theses&rft.isbn=9781109936124&rft.externalDBID=HAS_PDF_LINK&rft.externalDocID=1310423561 |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=9781109936124/lc.gif&client=summon&freeimage=true |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=9781109936124/mc.gif&client=summon&freeimage=true |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=9781109936124/sc.gif&client=summon&freeimage=true |