Modeling the profibrotic microenvironment in vitro: model validation

• Published in: bioRxiv
• Main Authors: Grigorieva, Olga, Basalova, Natalia, Uliana Dyachkova, Novoseletskaya, Ekaterina, Vigovskii, Maksim, Arbatskiy, Mikhail, Kulebyakina, Maria, Efimenko, Anastasia
• Format: Paper
• Language: English
• Published: Cold Spring Harbor Cold Spring Harbor Laboratory Press 12.12.2023
• Subjects: Actin; Adipose tissue; Ascorbic acid; Bioinformatics; Cell culture; Cell fate; Collagen (type I); Effector cells; Extracellular matrix; Fibrils; Fibroblast activation protein; Fibroblasts; Fibronectin; Fibrosis; Growth factors; Hepatocyte growth factor; Mesenchymal stem cells; Microenvironments; MicroRNAs; miRNA; Osteonectin; Secretion; Secretome; Smooth muscle; Stromal cells

Establishing the molecular and cellular mechanisms of fibrosis requires the development of validated and reproducible models. The complexity of in vivo models challenges the monitoring of an individual cell fate, in some cases making it impossible. However, the set of factors affecting cells in in v...