VAPOR Bioreactors for in vitro Vascularization of FRESH 3D Bioprinted Collagen Tissues
In recent decades, the field of regenerative medicine has aimed to generate patient-specific tissues to treat various diseases, injuries, and improve drug development. Unfortunately, most engineering strategies are limited to thin 2D cell sheets that have resulted in minimal translational success. T...
Saved in:
Main Author | |
---|---|
Format | Dissertation |
Language | English |
Published |
ProQuest Dissertations & Theses
01.01.2022
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | In recent decades, the field of regenerative medicine has aimed to generate patient-specific tissues to treat various diseases, injuries, and improve drug development. Unfortunately, most engineering strategies are limited to thin 2D cell sheets that have resulted in minimal translational success. The failure to generate large (≥ 1 cm3) tissues stems from the inability to generate microvasculature for nutrient delivery which constrains tissue thickness to the limits of passive nutrient diffusion (~250 μm). This work aims to bridge the macro (> 1 mm) and the micro (< 1 mm)length scales of vascular engineering to create true multi-scale vasculature. Using freeform reversible embedding of suspended hydrogels (FRESH), I 3D bioprint millimeter-scale collagen vessels and integrate them with micron-scale cell-assembled capillaries. To achieve integration of the engineered vessels with cell-assembled capillaries, I developed a bioreactor system termed vasculature and perfusion organ-on-a-chip reactor (VAPOR) to perfuse FRESH printed collagen based high-resolution internally perfusable scaffolds (CHIPS). This dissertation examines how the VAPOR perfusion of CHIPS containing a porous cell-ECM hydrogel drives angiogenesis to generate multi-scale vascularized tissues in vitro. The result of this work is a highly modular tissue engineering platform that can produce larger microvascularized human tissues for next-generation organ-specific research, repair, and drug development. |
---|---|
AbstractList | In recent decades, the field of regenerative medicine has aimed to generate patient-specific tissues to treat various diseases, injuries, and improve drug development. Unfortunately, most engineering strategies are limited to thin 2D cell sheets that have resulted in minimal translational success. The failure to generate large (≥ 1 cm3) tissues stems from the inability to generate microvasculature for nutrient delivery which constrains tissue thickness to the limits of passive nutrient diffusion (~250 μm). This work aims to bridge the macro (> 1 mm) and the micro (< 1 mm)length scales of vascular engineering to create true multi-scale vasculature. Using freeform reversible embedding of suspended hydrogels (FRESH), I 3D bioprint millimeter-scale collagen vessels and integrate them with micron-scale cell-assembled capillaries. To achieve integration of the engineered vessels with cell-assembled capillaries, I developed a bioreactor system termed vasculature and perfusion organ-on-a-chip reactor (VAPOR) to perfuse FRESH printed collagen based high-resolution internally perfusable scaffolds (CHIPS). This dissertation examines how the VAPOR perfusion of CHIPS containing a porous cell-ECM hydrogel drives angiogenesis to generate multi-scale vascularized tissues in vitro. The result of this work is a highly modular tissue engineering platform that can produce larger microvascularized human tissues for next-generation organ-specific research, repair, and drug development. |
Author | Hudson, Andrew R |
Author_xml | – sequence: 1 givenname: Andrew surname: Hudson middlename: R fullname: Hudson, Andrew R |
BookMark | eNqNi08LgjAcQAcV9M_v8IPOwXLi9FimeCtMvMqyGQvZz7bZoU-fQR-g0zu895ZkqlHLCfFiHkcsjILQD-PdnHjWqiulNGaMBv6CVNX-fCrgoNBI0Tg0Flo0oDS8lDMIlbDN0Amj3sIp1IAtZEV6yYEdv1NvlHbyBgl2nbhLDaWydpB2TWat6Kz0flyRTZaWSb7tDT5H7-oHDkaPqvY5p9zfRTxk_1Ufy5lC-w |
ContentType | Dissertation |
Copyright | Database copyright ProQuest LLC; ProQuest does not claim copyright in the individual underlying works. |
Copyright_xml | – notice: Database copyright ProQuest LLC; ProQuest does not claim copyright in the individual underlying works. |
DBID | 050 053 0BH 0FC AAFGM ABUWG ADZZV AFKRA AGAJT AQTIP AZQEC BENPR CBPLH CCPQU DWQXO EU9 G20 M8- PIMPY PQCXX PQEST PQQKQ PQUKI PRINS |
DatabaseName | Dissertations & Theses Europe Full Text: Health & Medicine Dissertations & Theses Europe Full Text: Science & Technology ProQuest Dissertations and Theses Professional Dissertations & Theses @ Carnegie Mellon University ProQuest Central Korea - hybrid linking ProQuest Central (Alumni) ProQuest Central (Alumni) - hybrid linking ProQuest Central ProQuest Central Essentials - hybrid linking ProQuest Women's & Gender Studies - hybrid linking ProQuest Central Essentials AUTh Library subscriptions: ProQuest Central ProQuest Dissertations & Theses Global: The Sciences and Engineering Collection ProQuest One Community College ProQuest Central ProQuest Dissertations & Theses A&I ProQuest Dissertations & Theses Global ProQuest Dissertations and Theses A&I: The Sciences and Engineering Collection ProQuest - Publicly Available Content Database ProQuest Central - hybrid linking ProQuest One Academic Eastern Edition (DO NOT USE) ProQuest One Academic ProQuest One Academic UKI Edition ProQuest Central China |
DatabaseTitle | Publicly Available Content Database Dissertations & Theses Europe Full Text: Health & Medicine ProQuest Central Essentials ProQuest One Academic Eastern Edition ProQuest Central (Alumni Edition) ProQuest One Community College ProQuest Dissertations & Theses: Open Dissertations & Theses @ Carnegie Mellon University ProQuest Dissertations & Theses Global: The Sciences and Engineering Collection ProQuest Dissertations and Theses Professional ProQuest Central China ProQuest Dissertations and Theses A&I: The Sciences and Engineering Collection ProQuest Dissertations & Theses Global Dissertations & Theses Europe Full Text: Science & Technology ProQuest Central ProQuest One Academic UKI Edition ProQuest Central Korea ProQuest One Academic ProQuest Dissertations & Theses A&I |
DatabaseTitleList | Publicly Available Content Database |
Database_xml | – sequence: 1 dbid: BENPR name: AUTh Library subscriptions: ProQuest Central url: https://www.proquest.com/central sourceTypes: Aggregation Database |
DeliveryMethod | fulltext_linktorsrc |
Genre | Dissertation/Thesis |
GroupedDBID | 050 053 0BH 0FC 8R4 8R5 ABUWG AFKRA AZQEC BENPR CBPLH CCPQU DWQXO EU9 G20 M8- PIMPY PQEST PQQKQ PQUKI PRINS Q2X |
ID | FETCH-proquest_journals_27707218763 |
IEDL.DBID | BENPR |
ISBN | 9798368462691 |
IngestDate | Thu Oct 10 20:40:15 EDT 2024 |
IsOpenAccess | true |
IsPeerReviewed | false |
IsScholarly | false |
Language | English |
LinkModel | DirectLink |
MergedId | FETCHMERGED-proquest_journals_27707218763 |
OpenAccessLink | https://www.proquest.com/docview/2770721876?pq-origsite=%requestingapplication% |
PQID | 2770721876 |
PQPubID | 18750 |
ParticipantIDs | proquest_journals_2770721876 |
PublicationCentury | 2000 |
PublicationDate | 20220101 |
PublicationDateYYYYMMDD | 2022-01-01 |
PublicationDate_xml | – month: 01 year: 2022 text: 20220101 day: 01 |
PublicationDecade | 2020 |
PublicationYear | 2022 |
Publisher | ProQuest Dissertations & Theses |
Publisher_xml | – name: ProQuest Dissertations & Theses |
SSID | ssib000933042 |
Score | 3.980487 |
Snippet | In recent decades, the field of regenerative medicine has aimed to generate patient-specific tissues to treat various diseases, injuries, and improve drug... |
SourceID | proquest |
SourceType | Aggregation Database |
SubjectTerms | Bioengineering Biomedical engineering |
Title | VAPOR Bioreactors for in vitro Vascularization of FRESH 3D Bioprinted Collagen Tissues |
URI | https://www.proquest.com/docview/2770721876 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV3NS8MwFH9odxE9KCp-TAnotbg2aZKexLmVIjjHmGW30XQJ9NLOtfr3m1cyFAa75BISkkfyPn7vC-DRaqyKFUr5uTGRz4RRvuR2oCbKQ1bwPJCYO_w-4ekne1tECwe4NS6scssTO0a9qgvEyJ9CIbCUl_28z-svH7tGoXfVtdA4hF6IlZs86A3Hk-ls114_iUUsKbfSNuRxsMN4O2mSnMLx6J8X_AwOdHUOWfYy_ZiRYVlbNa7rgUOsPknKivyU7aYmmQsZdXmTpDYksdRLCR3hIsTnrPJIEAiwLKIi846izQU8JOP5a-pvT7J0r6dZ_t2VXoJX1ZW-AiIlV5qumOGxZmIgVaBNYGg-EDlHq-sa-vt2utk_fQtHIQb2d-BCH7x2863vrLht1b2j6S_EFod2 |
link.rule.ids | 312,786,790,791,21409,33768,43829,74648 |
linkProvider | ProQuest |
linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV3NS8MwFH_odlD0oKj4MTWg1-LapEl6EudWqm51jFp2K02XwC6trtW_36RkKAx2ySUkJI-X9_F77-UB3GuLVZBCCCdXyncIU8LhVA9Y-blHCpq73NQOT2IafZDXuT-3gFtt0yrXMrEV1IuqMBj5g8eY-cpLP97Hzy_HdI0y0VXbQmMXuiagpbm6OxjF09mmv34YsIBjqrWtRwN3Q_C22iQ8goPhvyj4MezI8gTS9Gn6PkODZaXNuLYHDtL2JFqW6GfZrCqU2pRRWzeJKoVCTb0I4aFZZPA5bTwiAwRoEVGipKVofQp34Sh5jpz1STLLPXX2d1d8Bp2yKuU5IM6pkHhBFA0kYX0uXKlchfM-y6nxui6gt22ny-3Tt7AXJZNxNn6J365g3zNJ_i3Q0INOs_qW11r1NuLG0vcXS-OKag |
linkToPdf | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV3fS8MwED60AxF9UFScTg3oa9napkn6JM6u1F-1jFn2Vpotgb60ulb_fpOSoTDYS15CQnIkd99dvssB3CnEyvGCc7uQ0rcxldxmRDWe9AsXL0jhMJ07_JaQ-AM_z_254T81hla51omdol7WCx0jH7qU6q-81OUdSkOLSMPo_vPL1hWk9EurKaexCz2KCWYW9MaTJJ1u-u6HAQ2YR5TldUngbCjhzrJER3AQ_nsRP4YdUZ1Alj2k71M0LmsF6bp6OEhhS1RW6KdsVzXKDH3U5FCiWqJISTJGXqgH6VidApJIBwWUuqjQrJNucwq30WT2GNvrleTmJDX53769M7CquhLngBgjXHhLLEkgMB0x7gjpSK8Y0YJoD6wPg20zXWzvvoE9Jdr89Sl5uYR9V_P9u5jDAKx29S2ulBVu-bUR7y8PLo6e |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adissertation&rft.genre=dissertation&rft.title=VAPOR+Bioreactors+for+in+vitro+Vascularization+of+FRESH+3D+Bioprinted+Collagen+Tissues&rft.DBID=050%3B053%3B0BH%3B0FC%3BAAFGM%3BABUWG%3BADZZV%3BAFKRA%3BAGAJT%3BAQTIP%3BAZQEC%3BBENPR%3BCBPLH%3BCCPQU%3BDWQXO%3BEU9%3BG20%3BM8-%3BPIMPY%3BPQCXX%3BPQEST%3BPQQKQ%3BPQUKI%3BPRINS&rft.PQPubID=18750&rft.au=Hudson%2C+Andrew+R&rft.date=2022-01-01&rft.pub=ProQuest+Dissertations+%26+Theses&rft.isbn=9798368462691&rft.externalDBID=HAS_PDF_LINK |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=9798368462691/lc.gif&client=summon&freeimage=true |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=9798368462691/mc.gif&client=summon&freeimage=true |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=9798368462691/sc.gif&client=summon&freeimage=true |