Tetracycline Resistant Genetic Determinants In MDR Staphylococcus Aureus Isolates From Tertiary Care Hospitals, Kathmandu

The study was conducted to evaluate the rate of multidrug resistant Staphylococcus aureus (MDRSA) and to determine the tetracycline resistant genetic determinants in the MDRSA. 2004 different clinical specimens were subjected to bacteriological investigation following standard protocol. 266 S. aureu...

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Bibliographic Details
Published inWebology Vol. 19; no. 3; pp. 2715 - 2723
Main Authors Ghimire, Lata, Banjara, Megha Raj, Abdulla, Abdel Kodose MH
Format Journal Article
LanguageEnglish
Published Tehran Dr. Alireza Noruzi, University of Tehran, Department of Library and Information Science 01.01.2022
Subjects
Antibiotics
Antimicrobial agents
Bacteria
Drug resistance
Genes
Nosocomial infections
Pathogens
Penicillin
Staphylococcus infections
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Summary:The study was conducted to evaluate the rate of multidrug resistant Staphylococcus aureus (MDRSA) and to determine the tetracycline resistant genetic determinants in the MDRSA. 2004 different clinical specimens were subjected to bacteriological investigation following standard protocol. 266 S. aureus were isolated and identified which were subjected to antimicrobial susceptibility testing using modified Kirby-Bauer's disc diffusion method following CLSI guidelines. Both the genomic and plasmid DNA extraction was done and quantified using Nanodrop. Conventional Polymerase Chain Reaction was carried out for the amplification of tetK and tetM genes using specific primers. The rate of S. aureus was found as 266 (13.3%). The rate of MDRSA was found 95 (35.7%). Among the 95 MDRSA isolates, the organism resistant to tetracycline with disc diffusion was found to be 26 (9.8 %). 18 isolates had antibiotic resistance genes either tetK or tetM. 2 samples with tetracycline resistant isolates displayed both the tet K and tet M genes Out of 26 tetracycline-resistant MDRSA isolates, 46.15% (12/26) were positive for tetK, and 23.07% (6/26) were detected to harbor tetM .Two types of determinants tet K and tet M has been recorded significantly.
ISSN:1735-188X