RESPONSE SURFACE METHODOLOGY BASED MEDIUM OPTIMIZATION FOR IMPROVEMENT OF CHOLESTEROL OXIDASE ENZYME PRODUCTION BY NEWLY ISOLATED STREPTOMYCES SP

Cholesterol oxidase is an important enzyme with great commercial value in many clinical and industrial processes, such as determination of cholesterol levels in food, serum and other clinical samples as well as its potential application, as a biocatalyst for bioconversion of sterol compounds and as...

Full description

Saved in:
Bibliographic Details
Published inJournal of microbiology, biotechnology and food sciences Vol. 10; no. 3; p. 374
Main Authors Nayera A.M. Abdelwahed, Gamal, Amira A, Ismail, Shaymaa A, Hassan, Amira A
Format Journal Article
LanguageEnglish
Published Nitra Faculty of Biotechnology and Food Sciences 01.12.2020
Subjects
Online AccessGet full text

Cover

Loading…
Abstract Cholesterol oxidase is an important enzyme with great commercial value in many clinical and industrial processes, such as determination of cholesterol levels in food, serum and other clinical samples as well as its potential application, as a biocatalyst for bioconversion of sterol compounds and as an insecticide. A Preliminary comparative study of cholesterol oxidase enzyme production from Streptomyces sp. strain NHIA_CH5 using six different media containing cholesterol in submerged fermentation was investigated. An increase in the production was detected after two days in all of the studied media with a maximum production of 23.7 U/mL, noticed in medium containing soluble starch, glucose, peptone, beef extract, MgSO4.7H2O, and NaCl. A classical (one-variable-at-a-time) method revealed that the elimination of MgSO4.7H2O and NaCl favored cholesterol oxidase enzyme production. Statistical optimization of the other components was studied using the screening method Plackett-Burman design followed by the modeling method of the central composite design (CCD). The results showed that beef extract and glucose are the variables that significantly influenced and considered as the most important factors for cholesterol oxidase production. Experiments of CCD based on response surface methodology determine the optimum concentrations of these significant medium components and revealed maximum cholesterol oxidase enzyme production (50.5 U/mL) at beef extract 55 g/L and glucose 40 g/L which represented 2- fold higher titers than acquired from the non-optimized medium.
AbstractList Cholesterol oxidase is an important enzyme with great commercial value in many clinical and industrial processes, such as determination of cholesterol levels in food, serum and other clinical samples as well as its potential application, as a biocatalyst for bioconversion of sterol compounds and as an insecticide. A Preliminary comparative study of cholesterol oxidase enzyme production from Streptomyces sp. strain NHIA_CH5 using six different media containing cholesterol in submerged fermentation was investigated. An increase in the production was detected after two days in all of the studied media with a maximum production of 23.7 U/mL, noticed in medium containing soluble starch, glucose, peptone, beef extract, MgSO4.7H2O, and NaCl. A classical (one-variable-at-a-time) method revealed that the elimination of MgSO4.7H2O and NaCl favored cholesterol oxidase enzyme production. Statistical optimization of the other components was studied using the screening method Plackett-Burman design followed by the modeling method of the central composite design (CCD). The results showed that beef extract and glucose are the variables that significantly influenced and considered as the most important factors for cholesterol oxidase production. Experiments of CCD based on response surface methodology determine the optimum concentrations of these significant medium components and revealed maximum cholesterol oxidase enzyme production (50.5 U/mL) at beef extract 55 g/L and glucose 40 g/L which represented 2- fold higher titers than acquired from the non-optimized medium.
Author Gamal, Amira A
Hassan, Amira A
Ismail, Shaymaa A
Nayera A.M. Abdelwahed
Author_xml – sequence: 1
  fullname: Nayera A.M. Abdelwahed
– sequence: 2
  givenname: Amira
  surname: Gamal
  middlename: A
  fullname: Gamal, Amira A
– sequence: 3
  givenname: Shaymaa
  surname: Ismail
  middlename: A
  fullname: Ismail, Shaymaa A
– sequence: 4
  givenname: Amira
  surname: Hassan
  middlename: A
  fullname: Hassan, Amira A
BookMark eNqNjN1Og0AUhDdGE6v2HU7iNcjfFrikcCibsByyu6j0ptGEXhAFLfZBfGM3xgdwbiaZmW9u2OU0TwNj4HuuzyM_ehjfX4-LG3iBDTw3dMM4csLEu2ArPwwTh_txcs3WyzJ6Vps0DjhfsW-FuqVGI-hOlVmOINFUVFBNux62mcbCJoXoJFBrhBT7zAhqoCQFQraKHlFiY4BKyCuqURtUVAM9i8KygM2-lwh2V3T5L7jtocGnugehqc6MvddGYWtI9jlq0O0duzq-vC3D-s9v2X2JJq-cj9P8eR6Wr8M4n0-TrQ4BjzdpwL0gDf-3-gEUtFMm
ContentType Journal Article
Copyright 2020. This work is published under https://creativecommons.org/licenses/by-nd/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.
Copyright_xml – notice: 2020. This work is published under https://creativecommons.org/licenses/by-nd/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.
DBID 3V.
7X2
8FE
8FG
8FH
8FK
ABJCF
ABUWG
AFKRA
ARAPS
ATCPS
AZQEC
BBNVY
BENPR
BGLVJ
BHPHI
BYOGL
CCPQU
DWQXO
GNUQQ
HCIFZ
L6V
LK8
M0K
M7P
M7S
P5Z
P62
PIMPY
PQEST
PQQKQ
PQUKI
PRINS
PTHSS
DOI 10.15414/jmbfs.2020.10.3.374-380
DatabaseName ProQuest Central (Corporate)
Agricultural Science Collection
ProQuest SciTech Collection
ProQuest Technology Collection
ProQuest Natural Science Collection
ProQuest Central (Alumni) (purchase pre-March 2016)
Materials Science & Engineering Collection
ProQuest Central (Alumni)
ProQuest Central
Advanced Technologies & Aerospace Collection
Agricultural & Environmental Science Collection
ProQuest Central Essentials
Biological Science Collection
ProQuest Central
Technology Collection
Natural Science Collection
East Europe, Central Europe Database
ProQuest One Community College
ProQuest Central Korea
ProQuest Central Student
SciTech Premium Collection
ProQuest Engineering Collection
Biological Sciences
Agriculture Science Database
Biological Science Database
Engineering Database
Advanced Technologies & Aerospace Database
ProQuest Advanced Technologies & Aerospace Collection
Publicly Available Content Database
ProQuest One Academic Eastern Edition (DO NOT USE)
ProQuest One Academic
ProQuest One Academic UKI Edition
ProQuest Central China
Engineering Collection
DatabaseTitle Agricultural Science Database
Publicly Available Content Database
ProQuest Central Student
Technology Collection
ProQuest Advanced Technologies & Aerospace Collection
ProQuest Central Essentials
ProQuest Central (Alumni Edition)
SciTech Premium Collection
ProQuest One Community College
ProQuest Natural Science Collection
ProQuest Central China
ProQuest Central
ProQuest Engineering Collection
Natural Science Collection
ProQuest Central Korea
Agricultural & Environmental Science Collection
Biological Science Collection
Engineering Collection
Advanced Technologies & Aerospace Collection
Engineering Database
ProQuest Biological Science Collection
ProQuest One Academic Eastern Edition
East Europe, Central Europe Database
Agricultural Science Collection
ProQuest Technology Collection
Biological Science Database
ProQuest SciTech Collection
Advanced Technologies & Aerospace Database
ProQuest One Academic UKI Edition
Materials Science & Engineering Collection
ProQuest One Academic
ProQuest Central (Alumni)
DatabaseTitleList Agricultural Science Database
Database_xml – sequence: 1
  dbid: 8FG
  name: ProQuest Technology Collection
  url: https://search.proquest.com/technologycollection1
  sourceTypes: Aggregation Database
DeliveryMethod fulltext_linktorsrc
Discipline Biology
EISSN 1338-5178
GroupedDBID 3V.
5VS
7X2
8FE
8FG
8FH
8FK
A8Z
ABDBF
ABJCF
ABUWG
ADBBV
AFKRA
ALMA_UNASSIGNED_HOLDINGS
ARAPS
ATCPS
AZQEC
BBNVY
BCNDV
BENPR
BGLVJ
BHPHI
BPHCQ
BYOGL
CCPQU
DWQXO
DYU
ECGQY
EOJEC
GNUQQ
GROUPED_DOAJ
GX1
HCIFZ
KQ8
L6V
LK8
M0K
M7P
M7S
M~E
OBODZ
OK1
OZF
P62
PIMPY
PQEST
PQQKQ
PQUKI
PRINS
PROAC
PTHSS
RNS
TUS
ID FETCH-proquest_journals_25769250293
IEDL.DBID 8FG
IngestDate Thu Oct 10 20:29:31 EDT 2024
IsDoiOpenAccess true
IsOpenAccess true
IsPeerReviewed false
IsScholarly true
Issue 3
Language English
LinkModel DirectLink
MergedId FETCHMERGED-proquest_journals_25769250293
OpenAccessLink https://www.proquest.com/docview/2576925029?pq-origsite=%requestingapplication%
PQID 2576925029
PQPubID 1966353
ParticipantIDs proquest_journals_2576925029
PublicationCentury 2000
PublicationDate 20201201
PublicationDateYYYYMMDD 2020-12-01
PublicationDate_xml – month: 12
  year: 2020
  text: 20201201
  day: 01
PublicationDecade 2020
PublicationPlace Nitra
PublicationPlace_xml – name: Nitra
PublicationTitle Journal of microbiology, biotechnology and food sciences
PublicationYear 2020
Publisher Faculty of Biotechnology and Food Sciences
Publisher_xml – name: Faculty of Biotechnology and Food Sciences
SSID ssj0000697255
Score 4.3586273
Snippet Cholesterol oxidase is an important enzyme with great commercial value in many clinical and industrial processes, such as determination of cholesterol levels...
SourceID proquest
SourceType Aggregation Database
StartPage 374
SubjectTerms Beef
Bioconversion
Cholesterol
Cholesterol oxidase
Comparative studies
Enzymes
Fermentation
Glucose
Insecticides
Optimization
Peptone
Peptones
Response surface methodology
Sodium chloride
Starch
Statistical methods
Title RESPONSE SURFACE METHODOLOGY BASED MEDIUM OPTIMIZATION FOR IMPROVEMENT OF CHOLESTEROL OXIDASE ENZYME PRODUCTION BY NEWLY ISOLATED STREPTOMYCES SP
URI https://www.proquest.com/docview/2576925029
Volume 10
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV3LTsJAFJ0oxMSN8RkfSG6i2_Joh7azMoVOBUM7DdMqZWNoSxcmggou_Az_2Jmh6MKEZdNH0juTc8_ce3IPQrdZKyWFSMOagYtMwzZOtamFM40URW5ZhjElyrTPD8x-jB_GnXFZcFuWssoNJiqgzheZrJE3JTEmIl_r5O7tXZOuUbK7Wlpo7KJqW7dMKemzvfvfGkvLJJagzKWARxpeN19e00JO6dblubVhNAwLa4bd-ofEKr14h-ig5IXgrBfyCO3M5sdob-0U-XWCvkWgQhZwCjweeU6Pgk-jPnNVGwi6DqcuyEmNsQ8sjAQgTVTtCcQZDwZ-OGKPamo_MA9k74hywWLZENh44Ip3gQaTxKcgnnNjpSqBbgIBfRomMOBs6Ah4Ax6NaBgxP-lRDjw8RTcejXp9bfNHz-W2XD7_BdE4Q5X5Yj47R2BmhS2tx0gnz3A7tUiep50sFzxBT3GO9QtU2_aly-23r9C-jPZaAVJDldXH5-xa5PFVWleLVUfVLg3Ckbhyk_gHx4maPg
link.rule.ids 314,780,784,12765,21388,27924,27925,33373,33744,43600,43805
linkProvider ProQuest
linkToHtml http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV1NT8JAEN0oxujF-Bk_UCfRawG7C2VPptCtrbbdhi1KuRDa0oOJoIIHf4b_2N1S9GDiuR9JZ5v33s7MzkPoOm0kNJc0rGGSpxppk0QbGyTVaJ5nhoHxmBamfX7QcvrkftAclAm3edlWucLEAqizWapy5HUljKnka53evr5pyjVKVVdLC411tEGwpG51Uty--8mxNFrUkJK5bOBRhtf155ckV1O6dbVvreEaNoiG240_SFzQi72LdkpdCOZyIffQ2mS6jzaXTpGfB-hLBirkgWAg-j3b7DLwWeRwqygDQccUzAI1qbHvAw8jCUjDIvcEco8Hrh_2-GMxtR-4Dap2xIRUsdwDPnAt-SywYBj7DOR9Vr_oKoFODAF78mJwBfdMCW8goh4LI-7HXSZAhIfoymZR19FWXzQqf8v56DeI-AhVprPp5BhBK83bynqMNrOU3CQGzbKkmWZSJ-gJyYh-gqr_ven0_8uXaMuJfG_kucHDGdpWkV92g1RRZfH-MTmXnL5ILoqF-wabLppz
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=RESPONSE+SURFACE+METHODOLOGY+BASED+MEDIUM+OPTIMIZATION+FOR+IMPROVEMENT+OF+CHOLESTEROL+OXIDASE+ENZYME+PRODUCTION+BY+NEWLY+ISOLATED+STREPTOMYCES+SP&rft.jtitle=Journal+of+microbiology%2C+biotechnology+and+food+sciences&rft.au=Nayera+A.M.+Abdelwahed&rft.au=Gamal%2C+Amira+A&rft.au=Ismail%2C+Shaymaa+A&rft.au=Hassan%2C+Amira+A&rft.date=2020-12-01&rft.pub=Faculty+of+Biotechnology+and+Food+Sciences&rft.eissn=1338-5178&rft.volume=10&rft.issue=3&rft.spage=374&rft_id=info:doi/10.15414%2Fjmbfs.2020.10.3.374-380