Genome-wide identification of novel genes involved in Corynebacteriales cell envelope biogenesis using Corynebacterium glutamicum as a model
Abstract Corynebacteriales are Actinobacteria that possess an atypical didermic cell envelope. One of the principal features of this cell envelope is the presence of a large complex made up of peptidoglycan, arabinogalactan and mycolic acids. This covalent complex constitutes the backbone of the cel...
Saved in:
Published in | bioRxiv |
---|---|
Main Authors | , , , , |
Format | Paper |
Language | English |
Published |
Cold Spring Harbor
Cold Spring Harbor Laboratory Press
29.09.2020
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | Abstract Corynebacteriales are Actinobacteria that possess an atypical didermic cell envelope. One of the principal features of this cell envelope is the presence of a large complex made up of peptidoglycan, arabinogalactan and mycolic acids. This covalent complex constitutes the backbone of the cell wall and supports an outer membrane, called mycomembrane in reference to the mycolic acids that are its major component. The biosynthesis of the cell envelope of Corynebacteriales has been extensively studied, in particular because it is crucial for the survival of important pathogens such as Mycobacterium tuberculosis and is therefore a key target for anti-tuberculosis drugs. In this study, we explore the biogenesis of the cell envelope of Corynebacterium glutamicum, a non-pathogenic Corynebacteriales, which can tolerate dramatic modifications of its cell envelope as important as the loss of its mycomembrane. For this purpose, we used a genetic approach based on genome-wide transposon mutagenesis. We developed a highly effective immunological test based on the use of anti-arabinogalactan antibodies that allowed us to rapidly identify bacteria exhibiting an altered cell envelope. A very large number (10,073) of insertional mutants were screened by means of this test, and 80 were finally selected, representing 55 different loci. Bioinformatics analyses of these loci showed that approximately 60% corresponded to genes already characterized, 63% of which are known to be directly involved in cell wall processes, and more specifically in the biosynthesis of the mycoloyl-arabinogalactan-peptidoglycan complex. We identified 22 new loci potentially involved in cell envelope biogenesis, 76% of which encode putative cell envelope proteins. A mutant of particular interest was further characterized and revealed a new player in mycolic acid metabolism. Because a large proportion of the genes identified by our study is conserved in Corynebacteriales, the library described here provides a new resource of genes whose characterization could lead to a better understanding of the biosynthesis of the envelope components of these bacteria. |
---|---|
AbstractList | Abstract Corynebacteriales are Actinobacteria that possess an atypical didermic cell envelope. One of the principal features of this cell envelope is the presence of a large complex made up of peptidoglycan, arabinogalactan and mycolic acids. This covalent complex constitutes the backbone of the cell wall and supports an outer membrane, called mycomembrane in reference to the mycolic acids that are its major component. The biosynthesis of the cell envelope of Corynebacteriales has been extensively studied, in particular because it is crucial for the survival of important pathogens such as Mycobacterium tuberculosis and is therefore a key target for anti-tuberculosis drugs. In this study, we explore the biogenesis of the cell envelope of Corynebacterium glutamicum, a non-pathogenic Corynebacteriales, which can tolerate dramatic modifications of its cell envelope as important as the loss of its mycomembrane. For this purpose, we used a genetic approach based on genome-wide transposon mutagenesis. We developed a highly effective immunological test based on the use of anti-arabinogalactan antibodies that allowed us to rapidly identify bacteria exhibiting an altered cell envelope. A very large number (10,073) of insertional mutants were screened by means of this test, and 80 were finally selected, representing 55 different loci. Bioinformatics analyses of these loci showed that approximately 60% corresponded to genes already characterized, 63% of which are known to be directly involved in cell wall processes, and more specifically in the biosynthesis of the mycoloyl-arabinogalactan-peptidoglycan complex. We identified 22 new loci potentially involved in cell envelope biogenesis, 76% of which encode putative cell envelope proteins. A mutant of particular interest was further characterized and revealed a new player in mycolic acid metabolism. Because a large proportion of the genes identified by our study is conserved in Corynebacteriales, the library described here provides a new resource of genes whose characterization could lead to a better understanding of the biosynthesis of the envelope components of these bacteria. |
Author | Houssin, Christine Tropis, Maryelle Constantinesco-Becker, Florence Célia De Sousa-D’auria Constant, Patricia |
Author_xml | – sequence: 1 fullname: Célia De Sousa-D’auria – sequence: 2 givenname: Florence surname: Constantinesco-Becker fullname: Constantinesco-Becker, Florence – sequence: 3 givenname: Patricia surname: Constant fullname: Constant, Patricia – sequence: 4 givenname: Maryelle surname: Tropis fullname: Tropis, Maryelle – sequence: 5 givenname: Christine surname: Houssin fullname: Houssin, Christine |
BookMark | eNqNi0FuwjAQRb2ARSkcoLuRWBNsR1RhjaAcgD0yySQa5MzQ2E7VO3DoWqgbdiy-_pP--zM1YWFU6sPowhht1lZbXehtYbdFaSpd6jd1_0KWHlc_1CDkcKSWahdJGKQFlhE9dMgYgHgUP2KTAXYy_DJeXB1xIOfzWqP3gJx1uSFcSB4nCpACcfd8SD10PkXXU53RBXDQS4N-rqat8wEX__2ulof9aXdc3Qb5Thji-Spp4Dyd7UZXxn5aW5WvWX_AP1f3 |
ContentType | Paper |
Copyright | 2020. This article is published under http://creativecommons.org/licenses/by/4.0/ (“the License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. |
Copyright_xml | – notice: 2020. This article is published under http://creativecommons.org/licenses/by/4.0/ (“the License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. |
DBID | 8FE 8FH AAFGM AAMXL ABOIG ABUWG ADZZV AFKRA AFLLJ AFOLM AGAJT AQTIP AZQEC BBNVY BENPR BHPHI CCPQU DWQXO GNUQQ HCIFZ LK8 M7P PIMPY PQCXX PQEST PQQKQ PQUKI PRINS |
DOI | 10.1101/2020.09.29.318030 |
DatabaseName | ProQuest SciTech Collection ProQuest Natural Science Collection ProQuest Central Korea - hybrid linking Natural Science Collection - hybrid linking Biological Science Collection - hybrid linking ProQuest Central (Alumni) ProQuest Central (Alumni) - hybrid linking ProQuest Central UK/Ireland SciTech Premium Collection - hybrid linking ProQuest Central Student - hybrid linking ProQuest Central Essentials - hybrid linking ProQuest Women's & Gender Studies - hybrid linking ProQuest Central Essentials Biological Science Collection AUTh Library subscriptions: ProQuest Central ProQuest Natural Science Collection ProQuest One Community College ProQuest Central ProQuest Central Student SciTech Premium Collection (Proquest) (PQ_SDU_P3) Biological Sciences Biological Science Database Publicly Available Content Database ProQuest Central - hybrid linking ProQuest One Academic Eastern Edition (DO NOT USE) ProQuest One Academic ProQuest One Academic UKI Edition ProQuest Central China |
DatabaseTitle | Publicly Available Content Database ProQuest Central Student ProQuest Biological Science Collection ProQuest Central Essentials ProQuest One Academic Eastern Edition ProQuest Central (Alumni Edition) SciTech Premium Collection ProQuest One Community College ProQuest Natural Science Collection Biological Science Database ProQuest SciTech Collection ProQuest Central China ProQuest Central ProQuest One Academic UKI Edition Natural Science Collection ProQuest Central Korea Biological Science Collection ProQuest One Academic |
DatabaseTitleList | Publicly Available Content Database |
Database_xml | – sequence: 1 dbid: BENPR name: AUTh Library subscriptions: ProQuest Central url: https://www.proquest.com/central sourceTypes: Aggregation Database |
DeliveryMethod | fulltext_linktorsrc |
Genre | Working Paper/Pre-Print |
GroupedDBID | 8FE 8FH ABUWG AFKRA AZQEC BBNVY BENPR BHPHI CCPQU DWQXO GNUQQ HCIFZ LK8 M7P PIMPY PQEST PQQKQ PQUKI PRINS |
ID | FETCH-proquest_journals_25081262283 |
IEDL.DBID | BENPR |
IngestDate | Thu Oct 10 20:05:30 EDT 2024 |
IsDoiOpenAccess | true |
IsOpenAccess | true |
IsPeerReviewed | false |
IsScholarly | false |
Language | English |
LinkModel | DirectLink |
MergedId | FETCHMERGED-proquest_journals_25081262283 |
OpenAccessLink | https://www.proquest.com/docview/2508126228?pq-origsite=%requestingapplication% |
PQID | 2508126228 |
PQPubID | 2050091 |
ParticipantIDs | proquest_journals_2508126228 |
PublicationCentury | 2000 |
PublicationDate | 20200929 |
PublicationDateYYYYMMDD | 2020-09-29 |
PublicationDate_xml | – month: 09 year: 2020 text: 20200929 day: 29 |
PublicationDecade | 2020 |
PublicationPlace | Cold Spring Harbor |
PublicationPlace_xml | – name: Cold Spring Harbor |
PublicationTitle | bioRxiv |
PublicationYear | 2020 |
Publisher | Cold Spring Harbor Laboratory Press |
Publisher_xml | – name: Cold Spring Harbor Laboratory Press |
Score | 3.2973156 |
Snippet | Abstract Corynebacteriales are Actinobacteria that possess an atypical didermic cell envelope. One of the principal features of this cell envelope is the... |
SourceID | proquest |
SourceType | Aggregation Database |
SubjectTerms | Acids Arabinogalactan Bacteria Bioinformatics Biosynthesis Cell walls Corynebacterium glutamicum Genomes Mutants Mycolic acids Peptidoglycans Transposon mutagenesis Tuberculosis |
Title | Genome-wide identification of novel genes involved in Corynebacteriales cell envelope biogenesis using Corynebacterium glutamicum as a model |
URI | https://www.proquest.com/docview/2508126228 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV1LSwMxEA7aXrwpKj6qDOg1uM-6exIsrUWwFFHorSTNbFmw2dptK_4Hf7QzMUX00FsgZFmG4fvm8SUjxLUJDQUJJpTxJA0kx-hSm6KQmrhOUYSSx4rvOz8N2v3X5HGUjnzBrfayyg0mOqA21YRr5DdE1cRF7SjK7ubvkqdGcXfVj9DYFc0oTLhN27zvDobPvn1J7sbJfcAvmUY5paeZUzv_A13HJL190RyqOS4OxA7aQ_H1gLaaofwoDUJpvHLHGQuqAmy1xjeYMhxBaQlI1mhoAZ1q8WlR_zy0TAhfA9ffAa0TACHosnKHyhpY1z79e2A1gyn5Gw-ip6WqQYGbh3Mkrnrdl05fbn587B2tHv-aJT4WDVtZPBGQZ0loEkRKTVQSqECbdpHeosqTWKeoglPR2vals-3b52KPjcqiiShvicZyscILYualvvTm_wbwi5jx |
link.rule.ids | 786,790,21416,27956,33777,43838 |
linkProvider | ProQuest |
linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV1NSwMxEA3aHvSmqPhRdUCvwe1-1N2TYGmt2pYiFXorSTNbFmy27rYV_4M_2kxMET30FliyLLPDezOTlxnGrlVdmSBB1XkwiTxOMTqXKk25NFwnTISSBILuO_f6jc5r-DSKRq7gVjpZ5RoTLVCrfEI18htD1YaLGr4f383fOU2NotNVN0Jjm1Wp5WZcYdX7Vn_w4o4vjbtRcu9RJ1M_MelpbNXO_0DXMkl7j1UHYo7FPttCfcC-HlDnM-QfmULIlFPuWGNBnoLOV_gGU4IjyLQBkhUqs4BmXnxqlD-Nlg3Cl0D1d0BtBUAIMsvtpqwE0rVP_25YzmBq_I0G0ZulKEGAnYdzyK7arWGzw9cfPnaOVo5_zRIcsYrONR4zSOKwrkJEk5qI0BOeVI00ukWRhIGMUHgnrLbpTaebH1-ync6w1x13H_vPZ2yXDEwCCj-pscqiWOK5YemFvHC_4htOQZvn |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Genome-wide+identification+of+novel+genes+involved+in+Corynebacteriales+cell+envelope+biogenesis+using+Corynebacterium+glutamicum+as+a+model&rft.jtitle=bioRxiv&rft.au=C%C3%A9lia+De+Sousa-D%E2%80%99auria&rft.au=Constantinesco-Becker%2C+Florence&rft.au=Constant%2C+Patricia&rft.au=Tropis%2C+Maryelle&rft.date=2020-09-29&rft.pub=Cold+Spring+Harbor+Laboratory+Press&rft_id=info:doi/10.1101%2F2020.09.29.318030 |