Promotion of USP4 to TGF-β1-induced EMT in renal tubular epithelial cells is regulated by Akt through stabilizing TβRI

• Published in: bioRxiv
• Main Authors: Jin-Yun, Pu, Li-Xia, Wang, Wang, Jie, Zhang, Yu, Zhou, Jian-Hua
• Format: Paper
• Language: English
• Published: Cold Spring Harbor Cold Spring Harbor Laboratory Press 28.10.2020
• Subjects: 1-Phosphatidylinositol 3-kinase; AKT protein; Cytoplasm; E-cadherin; Epithelial cells; Fibrosis; Immunohistochemistry; Kidneys; Mesenchyme; Peptidase; Phosphorylation; Protein expression; siRNA; Transforming growth factor-b1; Ubiquitin

Abstract Objective We aimed to explore the role of ubiquitin-specific peptidase-4 (USP4) in TGF-β1 induced epithelial-mesenchymal transition (EMT) during renal fibrosis, and investigated that if Akt inactivation exerted a critical effect on EMT via USP4/TβRI pathway. Methods USP4, pAkt and TβRI prot...